2005
DOI: 10.1242/jcs.02356
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Exchange of clathrin, AP2 and epsin on clathrin-coated pits in permeabilized tissue culture cells

Abstract: Clathrin and clathrin adaptors on clathrin-coated pits exchange with cytosolic clathrin and clathrin adaptors in vivo. This exchange might require the molecular chaperone Hsc70 and J-domain-protein auxilin, which, with ATP, uncoat clathrin-coated vesicles both in vivo and in vitro. We find that, although Hsc70 and ATP alone could not uncoat clathrin-coated pits, further addition of auxilin caused rapid uncoating of clathrin but not AP2 and epsin. By contrast, cytosol uncoats clathrin, AP2 and epsin from pits i… Show more

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Cited by 27 publications
(29 citation statements)
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“…Full-length GAK was found to bind most tightly to monophosphorylated PIs, notably PI4P Massol et al 2006), which prompted the hypothesis that conversion of PIP2 to PI4P by a PI5 0 phosphatase might act as the trigger recruiting GAK to newly scissioned vesicles (Massol et al 2006). Indeed it was already known that the 5 0 -PI phosphatase synaptojanin is required for CCV uncoating (Verstreken et al 2003) and knockout in mice of auxilin1 þ 2 (Yim et al 2005;Lee et al 2008) or synaptojanin (Cremona et al 1999) similarly led to accumulation of synaptic CCVs. Loss of PIP2 from the newly scissioned vesicle via synaptojanin-mediated PIP2 dephosphorylation would explain why CCVs also lose their AP2 shell because binding of AP2 to the membrane is mediated by PIP2 (Honing et al 2005).…”
Section: Uncoatingmentioning
confidence: 99%
“…Full-length GAK was found to bind most tightly to monophosphorylated PIs, notably PI4P Massol et al 2006), which prompted the hypothesis that conversion of PIP2 to PI4P by a PI5 0 phosphatase might act as the trigger recruiting GAK to newly scissioned vesicles (Massol et al 2006). Indeed it was already known that the 5 0 -PI phosphatase synaptojanin is required for CCV uncoating (Verstreken et al 2003) and knockout in mice of auxilin1 þ 2 (Yim et al 2005;Lee et al 2008) or synaptojanin (Cremona et al 1999) similarly led to accumulation of synaptic CCVs. Loss of PIP2 from the newly scissioned vesicle via synaptojanin-mediated PIP2 dephosphorylation would explain why CCVs also lose their AP2 shell because binding of AP2 to the membrane is mediated by PIP2 (Honing et al 2005).…”
Section: Uncoatingmentioning
confidence: 99%
“…Transfected GFP-clathrin on the plasma membrane was imaged using TIR-FM in mock-depleted (e) and GAK-depleted (f) cells. both during clathrin uncoating and clathrin exchange Umeda et al, 2000;Yim et al, 2005).…”
Section: Dynamic Properties Of Free and Bound Clathrin In Gakdepletedmentioning
confidence: 99%
“…Cells were maintained in Dulbecco's modified Eagle's medium (DMEM), 10% fetal bovine serum, 2 mM glutamine, and 1% penicillin-streptomycin in 75 cm 2 culture bottles in a 5% CO 2 atmosphere at 37°C. CHO cells were cultured and seeded as described previously (Yim et al, 2005).…”
Section: Cell Culturementioning
confidence: 99%