Excellent Stability and Synthetic Activity of Lipase from<i>B. Stearothermophilus</i>MC7 Immobilized on Tin Dioxide in Environmentally Friendly Medium

Guncheva, Maya; Dimitrov, Momtchil; Kambourova, Margarita

doi:10.5504/bbeq.2013.0010

Cited by 6 publications

(1 citation statement)

References 48 publications

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“…All studied additives slowed down the process of thermal denaturation; an increased enzyme stability was observed for a large number of modifiers applied within a broad concentration range with the highest degree of activation when PEG 6000 was applied (up to 2.3-fold increase) [43]. A beneficial effect on the operational and thermal stability of lipase MC7 immobilized on nanostructured tin dioxide was observed in two ionic liquids [44]. Stearyl stearate production was achieved at a much higher reaction rate in the presence of 3methyl-1-octyl imidazolium chloride compared to solvent-free medium and 95% conversion of the substrates was achieved for 5 h at 65°C.…”

Section: Lipase Produced By G Stearothermophilus Mc7mentioning

confidence: 92%

Thermostable enzymes and polysaccharides produced by thermophilic bacteria isolated from Bulgarian hot springs

Kambourova

2018

Engineering in Life Sciences

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Thermostable enzymes (thermozymes) have been recognized as extremophilic compounds with a greatest biotechnological importance in different industrial areas. Quite recently exopolysaccharides (EPSs) synthesized by thermophiles became an object of increased research interest due to their unique properties appropriate for some specific industrial needs. Thermophilic producers of biotechnologically valuable enzymes and novel EPS were isolated by our group from Bulgarian thermal springs with a diverse geotectonic origin and different water properties. Laboratory reactor processes for their production were developed in batch and continuous cultures. Some of the synthesized thermostable enzymes were among the first described in their groups, for example, the single known thermostable gellan lyase that demonstrated specific activity higher than that of the mesophilic enzymes. Isolated by us thermostable xylanase was able to degrade more than 60% of beechwood xylan in a coprocess with an archaeal β‐xylosidase. Lipase purified by us was active between 55 and 90°C with an optimum at 75–80°C in a large pH range. It was able to degrade a broad range of substrates. Isolates from Bulgarian hot springs synthesized EPS with novel composition and high thermostability. Thus, Bulgarian hot springs harbor a wide set of thermophilic producers of novel enzymes and EPS with potential for a large number of biotechnological applications.

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Section: Lipase Produced By G Stearothermophilus Mc7mentioning

confidence: 92%

Thermostable enzymes and polysaccharides produced by thermophilic bacteria isolated from Bulgarian hot springs

Kambourova

2018

Engineering in Life Sciences

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Production of alkyl esters from macaw palm oil by a sequential hydrolysis/esterification process using heterogeneous biocatalysts: optimization by response surface methodology

Bressani

Garcia

Hirata

et al. 2014

Bioprocess Biosyst Eng

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The present study deals with the enzymatic synthesis of alkyl esters with emollient properties by a sequential hydrolysis/esterification process (hydroesterification) using unrefined macaw palm oil from pulp seeds (MPPO) as feedstock. Crude enzymatic extract from dormant castor bean seeds was used as biocatalyst in the production of free fatty acids (FFA) by hydrolysis of MPPO. Esterification of purified FFA with several alcohols in heptane medium was catalyzed by immobilized Thermomyces lanuginosus lipase (TLL) on poly-hydroxybutyrate (PHB) particles. Under optimal experimental conditions (mass ratio oil:buffer of 35% m/m, reaction temperature of 35 °C, biocatalyst concentration of 6% m/m, and stirring speed of 1,000 rpm), complete hydrolysis of MPPO was reached after 110 min of reaction. Maximum ester conversion percentage of 92.4 ± 0.4% was reached using hexanol as acyl acceptor at 750 mM of each reactant after 15 min of reaction. The biocatalyst retained full activity after eight successive cycles of esterification reaction. These results show that the proposed process is a promising strategy for the synthesis of alkyl esters of industrial interest from macaw palm oil, an attractive option for the Brazilian oleochemical industry.

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Characterization and Application of Yarrowia lipolytica Lipase Obtained by Solid-State Fermentation in the Synthesis of Different Esters Used in the Food Industry

Souza

Ribeiro

Coelho

2019

Appl Biochem Biotechnol

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Excellent Stability and Synthetic Activity of Lipase fromB. StearothermophilusMC7 Immobilized on Tin Dioxide in Environmentally Friendly Medium

Cited by 6 publications

References 48 publications

Thermostable enzymes and polysaccharides produced by thermophilic bacteria isolated from Bulgarian hot springs

Thermostable enzymes and polysaccharides produced by thermophilic bacteria isolated from Bulgarian hot springs

Production of alkyl esters from macaw palm oil by a sequential hydrolysis/esterification process using heterogeneous biocatalysts: optimization by response surface methodology

Characterization and Application of Yarrowia lipolytica Lipase Obtained by Solid-State Fermentation in the Synthesis of Different Esters Used in the Food Industry

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