Excellent N-fixing and P-solubilizing traits in earthworm gut-isolated bacteria: A vermicompost based assessment with vegetable market waste and rice straw feed mixtures

Hussain, Nazneen; Singh, Archana; Saha, Swati; Mattaparthi, Venkata Satish Kumar; Bhattacharyya, Pradip; Bhattacharya, Suvendu

doi:10.1016/j.biortech.2016.09.115

Cited by 100 publications

(16 citation statements)

References 31 publications

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“…Three of them (A2, B1 and B2) exhibited rod shape, which strongly suggested that they are bacilli ( Table 2). These findings indicated that the isolates might be NFB or PSB, as several researchers claimed that both the N-fixing and Psolubilizing bacteria display the gram negative colour and are rodshaped [7,8]. The finding of Sharma [10] in his study of Psolubilizing microorganism from soil also concurred with the present results, thus supporting that the bacteria isolates here might be PSB.…”

Section: Isolation and Identification Of Isolatessupporting

confidence: 90%

“…After starvation, the earthworms were disinfected with 70% ethanol for 30 s and blended. The gut content was suspended in 10 mL of sterile 0.85% NaCl solution and vigorously stirred for 15 min [7]. The gut content mixture was then diluted by using serial dilution technique.…”

Section: Isolation Of N-fixing and P-solubilizing Microbe From Earthwmentioning

confidence: 99%

“…However, most soil phosphorus, around 95 to 99 percent, is present in the form of insoluble phosphates and hence cannot be utilized by plants [15]. Several studies have been carried out to investigate the ability of bacterial species from the earthworms' gut to solubilize the insoluble phosphate compounds, such as tricalcium phosphate of Pikovskaya's medium [12] [7]. The phosphorus solubilizing activity is determined by the ability of microbes to release metabolites such as organic acids, which through their hydroxyl and carboxyl groups chelate the cation bound to phosphate, with the latter being converted to soluble forms [9].…”

Section: Qualitative Analysis Of P-solubilizing Bacteriamentioning

confidence: 99%

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Isolation and Characterization of Bacteria from Earthwormsâ€™ Intestines

Shaarani¹,

Jia²,

Arshad³

et al. 2018

IJET

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Vermicomposting; breaking down of organic material by earthworms that feed on wastes and converts them to soil-like mass and liquid, is an alternative to reduce waste into the environment. Nitrogen fixing and Phosphorus solubilizing bacteria in the earthworms’ intestines are important in promoting plant growth by increasing the Nitrogen (N) and Phosphorus (P) uptake when used as biofertilizers. The objectives of this study are to isolate, identify and characterize N-fixing and P-solubilizing bacteria from the earthworms’ intestines. The preparation of vermibeds included the introduction of leachate taken from Sungai Ikan Landfill, Kuala Terengganu. Standard serial dilution procedure was performed to isolate the microorganisms. Next, identification and characterization of bacteria were conducted via gram staining and bacteria morphological characteristic studies. Qualitative screening of N-fixing bacteria was performed by Hach Method 8039 while screening of P-solubilizing bacteria used Pikovskaya’s Agar containing insoluble tri-calcium phosphate (TCP). Six isolates, (A1, A2, B1, B2, C1 and D1) were successfully isolated. However, only single colony of A1, A2, B1 and B2 were obtained. All were gram negative and bacilli except for A1. A2 showed the highest nitrogen fixing and phosphate solubilizing activity though with the nitrate content (148.6 mg/L) and high phosphate solubilization efficiency (10.6).

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Section: Isolation and Identification Of Isolatessupporting

confidence: 90%

Section: Isolation Of N-fixing and P-solubilizing Microbe From Earthwmentioning

confidence: 99%

Section: Qualitative Analysis Of P-solubilizing Bacteriamentioning

confidence: 99%

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Isolation and Characterization of Bacteria from Earthwormsâ€™ Intestines

Shaarani¹,

Jia²,

Arshad³

et al. 2018

IJET

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“…It is generated by a process called vermicomposting, which consists of bio‐oxidation and transformation of organic matter (OM) through the combined action of worms ( Eisenia fetida ) and microorganisms under aerobic and mesophilic conditions (Kiyasudeen, Ibrahin, Quaik, & Ismail, 2016). Furthermore, N‐fixing and P‐solubilizing bacteria, which improve the nutritional characteristics of vermicompost, have been isolated from the gut of E. fetida (Hussain et al., 2016). In this context, new soilless production techniques must be improved and implemented with consideration of the particular characteristics of a substrate in a container with limited volumes of water, air, and nutrients available to the plant (Bowman & Paul, 1983).…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, N-fixing and P-solubilizing bacteria, which improve the nutritional characteristics of vermicompost, have been isolated from the gut of E. fetida (Hussain et al, 2016). In this context, new soilless production techniques must be improved and implemented with consideration of the particular characteristics of a substrate in a container with limited volumes of water, air, and nutrients available to the plant (Bowman & Paul, 1983).…”

mentioning

confidence: 99%

Evaluation of physicochemical properties and enzymatic activity of organic substrates during four crop cycles in soilless containers

Guerra

Sanjuán

López

2018

Food Science & Nutrition

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BackgroundOrganic soilless production in containers requires substrates with appropriate physicochemical and biological properties to ensure that production is sustainable and profitable for several production cycles. The main objective of this study was to comprehensively evaluate these properties in three different mixtures of organic substrates (vermicompost [V] and coconut fibers [CF] in ratios 20V80CF, 40V60CF, 60V40CF) for four horticultural crop production cycles (PCs) using vermicompost tea (VT) as the main source of nutrients.ResultsReadily available water (25%) in the control treatment (20V80CF) was below the recommended limit, and dry bulk density (>450 g/L) surpassed the recommended limit in the 60V40CF treatment (p < 0.05). In terms of chemical properties, cations and anions in the saturated media extract decreased significantly to values below established optimal conditions. Furthermore, the substrates presented high enzymatic activity in successive production cycles (p < 0.05), including dehydrogenase (350–400 μg TFF g−1), acid phosphatase (4,700 μg p‐nitrophenol g−1 soil hr−1), and β‐glucosidase (1,200 μg p‐nitrophenol g−1 soil hr−1) activity during transformation from organic matter to inorganic compounds.ConclusionThe 40V60CF treatment presents adequate physicochemical and biological characteristics for reuse for more than four growing cycles when organic supplements are administered.

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Bacterial and fungal communities and contribution of physicochemical factors during cattle farm waste composting

Huhe

Jiang

et al. 2017

MicrobiologyOpen

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During composting, the composition of microbial communities is subject to constant change owing to interactions with fluctuating physicochemical parameters. This study explored the changes in bacterial and fungal communities during cattle farm waste composting and aimed to identify and prioritize the contributing physicochemical factors. Microbial community compositions were determined by high‐throughput sequencing. While the predominant phyla in the bacterial and fungal communities were largely consistent during the composting, differences in relative abundances were observed. Bacterial and fungal community diversity and relative abundance varied significantly, and inversely, over time. Relationships between physicochemical factors and microbial community compositions were evaluated by redundancy analysis. The variation in bacterial community composition was significantly related to water‐soluble organic carbon (WSOC), and pile temperature and moisture (p < .05), while the largest portions of variation in fungal community composition were explained by pile temperature, WSOC, and C/N (p < .05). These findings indicated that those parameters are the most likely ones to influence, or be influenced by the bacterial and fungal communities. Variation partitioning analyses indicated that WSOC and pile temperature had predominant effects on bacterial and fungal community composition, respectively. Our findings will be useful for improving the quality of cattle farm waste composts.

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Excellent N-fixing and P-solubilizing traits in earthworm gut-isolated bacteria: A vermicompost based assessment with vegetable market waste and rice straw feed mixtures

Cited by 100 publications

References 31 publications

Isolation and Characterization of Bacteria from Earthwormsâ€™ Intestines

Isolation and Characterization of Bacteria from Earthwormsâ€™ Intestines

Evaluation of physicochemical properties and enzymatic activity of organic substrates during four crop cycles in soilless containers

Bacterial and fungal communities and contribution of physicochemical factors during cattle farm waste composting

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