Examining acute and chronic effects of short- and long-chain fatty acids on peptide YY (PYY) gene expression, cellular storage and secretion in STC-1 cells

Hand, Katharine; Bruen, Christine M.; O’Halloran, Fiona; Panwar, Harsh; Calderwood, Danielle; Giblin, Linda; Green, Brian D.

doi:10.1007/s00394-012-0439-9

Cited by 25 publications

(35 citation statements)

References 45 publications

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“…In accordance with a previous study in GLUTag and STC-1 cells (Drucker et al 1994), insulin was not detected in any of the cell lines. Our results also confirm data from other studies regarding the presence of GLP-2 in GLUTag and STC-1 cells, and the ability of GLUTag and in particular STC-1 cells, to produce and secrete CCK (Cordier-Bussat et al 1997, Hand et al 2010, Hand et al 2013, Nemoz-Gaillard et al 1998, Sidhu et al 2000.…”

Section: Discussionsupporting

confidence: 82%

“…A few studies have reported measurable PYY storage in and secretion from STC-1 cells (Geraedts et al 2009, Hand et al 2013, but the study by Geraedts et al (2009) reports measured concentrations that either are below or at the lower level of detection as reported by the assay manufacturers. However, given that the other peptides measured in our study were present at very high levels, combined with the lower limit of detection sensitivity (< 1 pM) of the applied assays for PYY measurement in this study, we consider it unlikely that our results are erroneous.…”

Section: Discussionmentioning

confidence: 98%

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Peptide production and secretion in GLUTag, NCI-H716, and STC-1 cells: a comparison to native L-cells

Kuhre¹,

Albrechtsen²,

Deacon³

et al. 2016

Journal of Molecular Endocrinology

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ResearchPeptide production in GLP1-producing cell lines r e kuhre and others 56:3 (2016) 56, 201-211 Journal of Molecular Endocrinology 201-211Key Words AbstractGLUTag, NCI-H716, and STC-1 are cell lines that are widely used to study mechanisms underlying secretion of glucagon-like peptide-1 (GLP-1), but the extent to which they resemble native L-cells is unknown. We used validated immunoassays for 14 different hormones to analyze peptide content (lysis samples; n = 9 from different passage numbers) or peptide secretion in response to buffer (baseline), and after stimulation with 50 mM KCl or 10 mM glucose + 10 µM forskolin/3-isobutyl-1-methylxanthine (n = 6 also different passage numbers). All cell lines produced and processed proglucagon into GLP-1, GLP-2, glicentin, and oxyntomodulin in a pattern (prohormone convertase (PC)1/3 dependent) similar to that described for human gut. All three cell lines showed basal secretion of GLP-1 and GLP-2, which increased after stimulation. In contrast to freshly isolated murine L-cells, all cell lines also expressed PC2 and secreted large amounts of pancreatic glucagon. Neurotensin and somatostatin storage was low and secretion was not consistently increased by stimulation. STC-1 cells released more glucose-dependent insulinotropic polypeptide than GLP-1 at baseline (P < 0.01) and KCl elevated its secretion (P < 0.05). Peptide YY, which normally co-localizes with GLP-1 in distal L-cells, was not detected in any of the cell lines. GLUTag and STC-1 cells also expressed vasoactive intestinal peptide, but none expressed pancreatic polypeptide or insulin. GLUTag contained and secreted large amounts of CCK, while NCI-H716 did not store this peptide and STC-1 contained low amounts. Our results show that hormone production in cell line models of the L-cell has limited similarity to the natural L-cells.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 98%

Peptide production and secretion in GLUTag, NCI-H716, and STC-1 cells: a comparison to native L-cells

Kuhre¹,

Albrechtsen²,

Deacon³

et al. 2016

Journal of Molecular Endocrinology

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show abstract

“…In addition to CCK, STC-1 cells express and secrete a wide range of gut hormones known for their roles in metabolism, feeding and satiety, including glucose dependent insulinotropic polypeptide (GIP), peptide YY (PYY), pancreatic polypeptide, neurotensin and the proglucagon derived peptides [glucogon-like peptide-1 (GLP-1), glucogon-like peptide-2 (GLP-2) and oxyntomodulin] (Rindi et al 1990 ;Hand et al 2013 ). STC-1 cells secrete these hormones in response to a range of physiological stimuli, although levels may differ to native enteroendocrine cells (Reimann et al 2008 ).…”

Section: Features and Mechanismsmentioning

confidence: 99%

“…Interestingly, these substances do not appear to be GLP-1 stimulants in primary cultures of murine intestinal epithelium (Reimann et al 2008 ). Although PYY secretion has been less widely investigated, STC-1 cells reliably produce and secrete PYY in response to a variety of fatty acids including valeric acid, linoleic acid and conjugated linoleic acid 9,11 (Hand et al 2013 ). GIP expression and production by STC-1 cells is poor.…”

Section: Features and Mechanismsmentioning

confidence: 99%