Examination of the immunoglobulin classes involved in the serological response of pregnant sheep toAspergillus fumigatus

Abstract

Pregnant sheep inoculated with Aspergillus fumigatus conidia developed agglutinating and precipitating antibodies for mycelial antigens. The agglutinins were initially exclusively of the IgM class, but were later supplemented by IgG antibodies, although IgM production was usually sustained throughout the serological response. Precipitins active in the immunodiffusion test were of the IgG class. They developed later in the immune response than agglutinins and declined more rapidly. The precipitins and IgG agglutinins were more closely associated with recent active infection than IgM agglutinins.

Comparative study of serological tests for the diagnosis of equine aspergillosis

“…Unlike in human beings with mycotic infections, where serological analysis as a diagnostic tool has attracted much attention (Latgé 1995), there have only been a few reports of anti‐ Aspergillus antibodies detection in horses until recently (Dixon and Rowlands 1981, Moore and others 1993, Guillot and others 1997). In this study, detection of precipiting antibodies was proved to be inaccurate in diagnosing equine aspergillosis, with similar findings being reported in cattle (Corbel and Day 1978, Wiseman and others 1984). Enzyme immunoassays using complex antigenic mixtures were not useful either.…”