Examination of the effects of Campylobacter concisus zonula occludens toxin on intestinal epithelial cells and macrophages

Mahendran, Vikneswari; Liu, Fang; Riordan, Stephen M.; Grimm, Michael; Tanaka, Mark M.; Zhang, Li

doi:10.1186/s13099-016-0101-9

Cited by 43 publications

(43 citation statements)

References 39 publications

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“…Previous studies found that some oral C. concisus strains or their toxins were able to damage the intestinal epithelial barrier and induce intestinal epithelial production of proinflammatory cytokines using cell line models111213. These data suggest that translocation of enteric virulent C. concisus strains from the human oral cavity to the intestinal tract may cause enteric diseases in some individuals.…”

mentioning

confidence: 64%

“…The CRISPR proteins found in C. concisus strains do not seem to be related to CON_phi2 prophage that contains the zonula occludens toxin gene31. The C. concisus Zot was found to damage intestinal epithelial barrier and affect the function of macrophages and the zot gene was detected in C. concisus strains from both GS1 and GS2112336.…”

Section: Discussionmentioning

confidence: 97%

“…There was some evidence that C. concisus strains of these two genomospecies may have different pathogenic potential15161718192021. For example, strains invasive to intestinal epithelial cells were often found in GS21011. Despite these findings, there is a lack of understanding regarding these two C. concisus genomospecies at the genome level.…”

Section: Discussionmentioning

confidence: 99%

“…A number of studies reported a significantly higher detection of C. concisus by PCR in intestinal biopsies collected from patients with IBD as compared to controls4567. In addition to IBD, C. concisus was frequently isolated from diarrheal stool samples, suggesting its possible role in human diarrheal disease891011.…”

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confidence: 99%

“…Strains from the two C. concisus genomospecies appear to have different enteric pathogenic potentials. Oral C. concisus strains that were invasive to intestinal epithelial cells were found in Genomospecies 2 (GS2)1011. GS2 C. concisus strains were more often isolated from faecal samples collected from patients with bloody diarrhoea and they were more invasive to intestinal epithelial cells as compared to Genomospecies 1 (GS1) strains1516.…”

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confidence: 99%

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Genome analysis of Campylobacter concisus strains from patients with inflammatory bowel disease and gastroenteritis provides new insights into pathogenicity

Chung

Tay

Octavia

et al. 2016

Sci Rep

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Campylobacter concisus is an oral bacterium that is associated with inflammatory bowel disease. C. concisus has two major genomospecies, which appear to have different enteric pathogenic potential. Currently, no studies have compared the genomes of C. concisus strains from different genomospecies. In this study, a comparative genome analysis of 36 C. concisus strains was conducted including 27 C. concisus strains sequenced in this study and nine publically available C. concisus genomes. The C. concisus core-genome was defined and genomospecies-specific genes were identified. The C. concisus core-genome, housekeeping genes and 23S rRNA gene consistently divided the 36 strains into two genomospecies. Two novel genomic islands, CON_PiiA and CON_PiiB, were identified. CON_PiiA and CON_PiiB islands contained proteins homologous to the type IV secretion system, LepB-like and CagA-like effector proteins. CON_PiiA islands were found in 37.5% of enteric C. concisus strains (3/8) isolated from patients with enteric diseases and none of the oral strains (0/27), which was statistically significant. This study reports the findings of C. concisus genomospecies-specific genes, novel genomic islands that contain type IV secretion system and putative effector proteins, and other new genomic features. These data provide novel insights into understanding of the pathogenicity of this emerging opportunistic pathogen.

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confidence: 64%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Genome analysis of Campylobacter concisus strains from patients with inflammatory bowel disease and gastroenteritis provides new insights into pathogenicity

Chung

Tay

Octavia

et al. 2016

Sci Rep

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The efficacy of anti‐proteolytic peptide R7I in intestinal inflammation, function, microbiota, and metabolites by multi‐omics analysis in murine bacterial enteritis

Sun

Liu

et al. 2022

Bioengineering & Transla Med

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Increased antibiotic resistance poses a major limitation in tackling inflammatory bowel disease and presents a large challenge for global health care. Antimicrobial peptides (AMPs) are a potential class of antimicrobial agents. Here, we have designed the potential oral route for antimicrobial peptide R7I with anti-proteolytic properties to deal with bacterial enteritis in mice. The results revealed that R7I protected the liver and gut from damage caused by inflammation. RNA-Seq analysis indicated that R7I promoted digestion and absorption in the small intestine by upregulating transmembrane transporter activity, lipid and small molecule metabolic processes and other pathways, in addition to upregulating hepatic steroid biosynthesis and fatty acid degradation. For the gut microbiota, Clostridia were significantly reduced in the R7I-treated group, and Odoribacteraceae, an efficient isoalloLCA-synthesizing strain, was the main dominant strain, protecting the gut from potential pathogens. In addition, we further discovered that R7I reduced the accumulation of negative organic acid metabolites. Overall, R7I exerted better therapeutic and immunomodulatory potential in the bacterial enteritis model, greatly reduced the risk of disease onset, and provided a reference for the in vivo application of antimicrobial peptides.

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Cleavage of cell junction proteins as a host invasion strategy in leptospirosis

Kumari,

Yadav,

Sarkar

et al. 2024

Appl Microbiol Biotechnol

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Infection and invasion are the prerequisites for developing the disease symptoms in a host. While the probable mechanism of host invasion and pathogenesis is known in many pathogens, very little information is available on Leptospira invasion/pathogenesis. For causing systemic infection Leptospira must transmigrate across epithelial barriers, which is the most critical and challenging step. Extracellular and membrane-bound proteases play a crucial role in the invasion process. An extensive search for the proteins experimentally proven to be involved in the invasion process through cell junction cleavage in other pathogens has resulted in identifying 26 proteins. The similarity searches on the Leptospira genome for counterparts of these 26 pathogenesis-related proteins identified at least 12 probable coding sequences. The proteins were either extracellular or membrane-bound with a proteolytic domain to cleave the cell junction proteins. This review will emphasize our current understanding of the pathogenic aspects of host cell junction-pathogenic protein interactions involved in the invasion process. Further, potential candidate proteins with cell junction cleavage properties that may be exploited in the diagnostic/therapeutic aspects of leptospirosis will also be discussed. Key points • The review focussed on the cell junction cleavage proteins in bacterial pathogenesis • Cell junction disruptors from Leptospira genome are identified using bioinformatics • The review provides insights into the therapeutic/diagnostic interventions possible

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Examination of the effects of Campylobacter concisus zonula occludens toxin on intestinal epithelial cells and macrophages

Cited by 43 publications

References 39 publications

Genome analysis of Campylobacter concisus strains from patients with inflammatory bowel disease and gastroenteritis provides new insights into pathogenicity

Genome analysis of Campylobacter concisus strains from patients with inflammatory bowel disease and gastroenteritis provides new insights into pathogenicity

The efficacy of anti‐proteolytic peptide R7I in intestinal inflammation, function, microbiota, and metabolites by multi‐omics analysis in murine bacterial enteritis

Cleavage of cell junction proteins as a host invasion strategy in leptospirosis

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