Examination of proliferation of probiotic microbes by isoperibolic calorimetry

Schäffer, B.; Daróczi, L.; Keller, Beáta; Szakály, Zoltán; Lőrinczy, D.

doi:10.1007/s10973-012-2818-0

Cited by 4 publications

(1 citation statement)

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“…Taking into account previous studies in case of suspected diagnosis of septic or periprosthetic infection, it may be advantageous-due to the shorter duration-a DSC [20,23] or the isoperibolic calorimeter compared to conventional microbiological cultures for the purpose of rapid diagnosis and choice of targeted antibiotic therapy [26,24,[43][44][45][46][47]. Using DSC, we found that changes of thermal characteristics of human synovial fluid samples collected of patients with different grades of osteoarthritis have shown correlation with severity of arthritis [23].…”

Section: Discussionmentioning

confidence: 94%

Thermal analysis of synovial fluids in different stages of osteoarthritis and after bacterial infections

Dandé

Kocsis

Lőrinczy

2020

J Therm Anal Calorim

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The analysis of synovial fluid is an important method in diagnosing and handling septic arthritis. To achieve a quick diagnosis could be a great advantage in the therapy. The differential scanning calorimetry (DSC) proved to be a useful technique in the differential diagnosis of tumors using blood plasma or sera. The aim of this paper is to show up some characteristic thermal parameters in the diagnosis of different grades of arthritis, which are in good agreement with the severity of disease checked by conventional X-ray supported grading. To follow the effect of different bacterial strains, the synovial fluids were inoculated by three types of bacterial strains (with 103–105 CFU mL−1 concentrations) at 37 °C and stored trough 24 h. After that, they were denatured in 20–90 °C temperature range with 0.3 K min−1 scanning rate. The change in the maximum denaturation temperature (Tm) and calorimetric enthalpy (∆H) monitored the severity of sepsis and depended on the type of bacteria. The proliferation characteristics of bacteria should be strain specific. The synovial fluid samples inoculated with the most frequently occurring bacteria were monitored in isotherm mode (isoperibol calorimeter) at 37 °C up to the end of the proliferation. The isoperibolic scans clearly demonstrated specific, concentration-dependent representative curves in case of each bacterium (duration of proliferation, maximum of proliferation rates, etc.). Therefore, thermal analysis of human synovial fluid samples by DSC or isoperibolic calorimetry could be a useful tool in the staging of osteoarthritis and the diagnostics of septic arthritis.

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