Examination of coumarins, flavonoids and polysaccharopeptide for antibacterial activity

IN VITRO AND IN SILICO ANTIBACTERIAL ACTIVITY OF PRANGOS¬ FERULACEA (L.) Lindl AND PRANGOS ULOPTERA DC, AND THEIR MUTAGENICITY IN THE AMES TEST

“…Molecular docking is one of best bioinformatic tools for drug design and determination the mechanism of antimicrobial agents (Kumalo et al, 2015).In the present study, the docking between 10 known compounds from P.ferulacea and bacterial proteins has been done.The results showed that the antibacterial activity of pinens of this plant was significantly more than coumarins. DNAgyrase subunit B was the main target proteins of pinens.The α-pinen was more effective than β-pinen with lowest and highest Ki and ∆Gb values respectively.Pinens ( were the main target proteins of tested coumarins.Among the tested coumarins,osethole was most effective which followed by Pesoralen,Isoimpratorin, Terpinolen,Gosferol, δ-3-caren, P-cymene, and Myrcene respectively.Coumarins are secondary metabolites that occur naturally in several plant families and possess important pharmacological properties, including inhibition of oxidative stress and use as the fragrance in food and cosmetic products (Borges et al, 2014).The antibacterial activity of some coumarins such as osthol,imperatorin, isoimpinellin.arbutin,baicalin and naringin have been reported previously (Widelski et al, 2009;Ng et al, 1996).The mechanisms of antibacterial effect of these compounds have not well defined and the present study is the first investigation of the mechanism of antibacterial activity of coumarins and pinens.Despite the therapeutic advantages possessed by medicinal plants but some constituents of medicinal plants have been shown to be potentially mutagenic,toxic, teratogenic and carcinogenic (Gadano et al, 2006;Akinboro and Bakare, 2007).Therefore, these plants should be evaluated to better understand their safety.The Ames test is commonly used with plant extracts for possible gene mutation determination (Mortelmans et al, 2000).In this study the Ames test was carried out using methanolic extracts of flower, leaf, stem, root and seeds from P.ferulacea and P.uloptera obtained results have not shown any mutagenicity to TA98 for both studied plants. Some studies demonstrated that different pinens and coumarins have not any mutagenicity effect.…”

Activity of plant flavonoids against antibiotic-resistant bacteria

“…Many flavonoids have been reported to possess a variety of biological activities including antiallergic, antiinflammatory, antiviral, antiproliferative and anticarcinogenic activities, in addition to having effects on mammalian metabolism (Middleton and Kandaswami, 1993). Although there were several earlier reports indicating the antibacterial activity of a few flavonoids (Cai and Wu, 1996;el-Gammal and Mansour, 1986;Inamori et al, 1991;Ng et al, 1996;Osawa et al, 1992;Rocha et al, 1995;Ward et al, 1981;Zheng et al, 1996), the antibacterial properties of flavonoids at large has not been investigated. Tsuchiya et al (1996) and Iinuma et al (1994) have reported that tetrahydroxyflavanones and exiguaflavanone D were active against MRSA, however, the activity of flavonoids against other antibiotic-resistant bacteria has not been examined.…”

Influence of Baicalin on the Expression of Receptor Activator of Nuclear Factor-κB Ligand in Cultured Human Periodontal Ligament Cells

“…Baicalin is a fl avonoid purifi ed from the medicinal plant Scutellaria baicalensis Georgi which has been used in herbal formulas to treat infl ammatory diseases for thousands of years in traditional Chinese medicine. It has been reported that baicalin exhibits antibacterial, anti-infl ammatory and analgesic effects [12][13][14][15][16][17] and can inhibit nuclear factor-B activation [18] . More and more studies have been focusing on the effect of baicalin on human periodontal ligament (HPDL) cells [19][20][21][22][23] .…”