Exact mass measurement by Fourier transform mass spectrometry

Ledford, Edward B.; Ghaderi, Sahba.; White, Robert L.; Spencer, Robert B.; Kulkarni, Pramod; Wilkins, Charles L.; Gross, Michael L.

doi:10.1021/ac50053a021

Cited by 99 publications

(30 citation statements)

References 26 publications

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“…For a large ion population, the consequences of these repulsions are referred to as space-charge effects. Space-charge effects result in an overall radial outward force on the ion cloud, giving rise to an ion-number-dependent shift in the motional frequencies (27). For the detection of low mass-to-charge ions, the primary effect of this shift is the reduction of mass accuracy.…”

Section: Space-charge Effectsmentioning

confidence: 99%

Detection of high mass‐to‐charge ions by Fourier transform mass spectrometry

Holliman

Rempel

Gross

1994

Mass Spectrometry Reviews

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Section: Space-charge Effectsmentioning

confidence: 99%

Detection of high mass‐to‐charge ions by Fourier transform mass spectrometry

Holliman

Rempel

Gross

1994

Mass Spectrometry Reviews

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“…For example, in order to derive the unique elemental composition from the monoisotopic mass for masses 9500 Da, one needs accuracy at the sub-ppm level or better [5,6], which can currently only be achieved by FT-ICR MS. Although it is well known that the massresolving power of FT-ICR varies linearly with the applied magnetic field strength and inversely with m/z and pressure [7,8], it is less widely appreciated that the mass-resolving power of FT-ICR can be enhanced by up to a factor of 2 by phasing the magnitude-mode spectrum into an absorptionmode spectrum [9,10].…”

Section: Introductionmentioning

confidence: 99%

Phase Correction of Fourier Transform Ion Cyclotron Resonance Mass Spectra Using MatLab

Thompson

Orden

et al. 2011

J. Am. Soc. Mass Spectrom.

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FT-ICR mass spectrometry has been limited to magnitude mode for almost 40 years due to the data processing methods used. However, it is well known that phase correction of the data can theoretically produce an absorption-mode spectrum with a mass-resolving power that is as much as twice as high as conventional magnitude mode, and that it also improves the quality of the peak shape. Temporally dispersed frequency sweep excitation followed by a time delay before detection results in a steep quadratic variation in the signal phase with frequency. Viewing this, it is possible to find the correct phase function by performing a quadratic least squares fit, modified by iterating through phase cycles until the correct quadratic function is found. Here, we present a robust manual method to rotate these signals mathematically and generate a "phased" absorption-mode spectrum. The method can, in principle, be automated. Baseline correction is also included to eliminate the accompanying baseline drift. The resulting experimental FT-ICR absorption-mode spectra exhibit a resolving power that is at least 50% higher than that of the magnitude mode.

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“…frequency is not equal to the cyclotron frequency (o c ) because the electric field (E) that is used to trap ions in the ICR cell causes frequency shifts, and the effect of the E field is not covered by Equation 1 (Ledford et al, 1980;Marshall, Hendrickson, & Jackson, 1998). Furthermore, the electric field associated with the ions present in the cell also causes shifts in cyclotron frequency.…”

Section: Theory Of Mass Calibrationmentioning

confidence: 99%

“…Furthermore, the electric field associated with the ions present in the cell also causes shifts in cyclotron frequency. The latter effect, often referred to as the ''space-charge effect,'' grows in proportion to both the number of ions in the cell and the charge present on the ions (Ledford & McIver, 1976;Ledford et al, 1980;Francl et al, 1983;Jeffries, Barlow, & Dunn, 1983;Ledford, Rempel, & Gross, 1984b). Frequency shifts from space charge can be a serious problem as the number of ions stored in the trap often varies from one moment to the next or from one experiment to another.…”

Section: Theory Of Mass Calibrationmentioning

confidence: 99%

Accurate mass measurements by Fourier transform mass spectrometry

Zhang¹,

Rempel

Pramanik³

et al. 2004

Mass Spectrometry Reviews

121

133

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The capability for accurate mass measurements is an important attribute of Fourier transform mass spectrometry (FTMS). Unlike other instrumental methods in mass spectrometry, FTMS still offers significant opportunities to improve mass measurement accuracy (MMA), making it an area of research. This review covers the published literature in FTMS from the late 1970s to the present. We discuss the development and evolution of mass calibration that give accuracies in the low ppm range. We sketch the derivation and show the common foundation of these mass calibration procedures. We also describe the relation of mass calibration and the fundamentals of ion motion and space-charge effects, and we review efforts to improve the basic calibration procedure particularly those that correct for effects of space charge. The advent of matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) have opened the door for FTMS to be used for analyzing at high performance biopolymers, including proteins, oligodeoxynucleotides (ODNs), oligosaccharides, and synthetic polymers. We also discuss the utility of FTMS for accurate mass measurement in these areas and some practical ways to improve MMA. # 2004 Wiley Periodicals, Inc., Mass Spec Rev 24:286-309, 2005

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Exact mass measurement by Fourier transform mass spectrometry

Cited by 99 publications

References 26 publications

Detection of high mass‐to‐charge ions by Fourier transform mass spectrometry

Detection of high mass‐to‐charge ions by Fourier transform mass spectrometry

Phase Correction of Fourier Transform Ion Cyclotron Resonance Mass Spectra Using MatLab

Accurate mass measurements by Fourier transform mass spectrometry

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