Ex Vivo Pretreatment with Melatonin Improves Survival, Proangiogenic/Mitogenic Activity, and Efficiency of Mesenchymal Stem Cells Injected into Ischemic Kidney

Abstract

ABSTRACT

14S,21R-dihydroxy-docosahexaenoic Acid Treatment Enhances Mesenchymal Stem Cell Amelioration of Renal Ischemia/Reperfusion Injury

“…We found that I/R injury increased serum creatinine compared with sham at day 1 (1.2 -0.06 vs. 0.35 -0.04 mg/dL, P < 0.01) and day 3 (1.08 -0.14 vs. 0.35 -0.04 mg/dL, P < 0.05) post-I/R (Fig. 1B), which is in agreement with previous reports [8,51]. Mice transplanted with mMSCs exhibited lower serum creatinine levels than the I/R control at day 1 (0.98 -0.06 mg/dL, P = 0.08) and day 3 (0.6 -0.05 mg/dL, P < 0.05) post-I/R, which is also consistent with a previous report [8].…”

“…In particular, MSCs can ameliorate renal ischemia/ reperfusion (I/R) injury [5][6][7][8][9][10]. It has been reported that MSCs repair renal damage by differentiating into tubular epithelial cells [11], mesangial cells [12], endothelial cells [13], and podocytes [14], and by producing renotrophic cytokines and growth factors [15][16][17][18][19].…”

“…Although MSCs possess the capability to ameliorate acute kidney injury, the low viability of transplanted cells in I/R injured organs remains an important limitation for MSC therapy [2,27]. Reactive oxygen species (ROS) and inflammatory cytokines generated in I/R are the probable source of damage to the grafted MSCs [8,20]. Several remedial approaches have been recommended to overcome MSC apoptosis [2], including ischemic or cytokine preconditioning and ex vivo genetic modification of stem cells.…”

“…Several remedial approaches have been recommended to overcome MSC apoptosis [2], including ischemic or cytokine preconditioning and ex vivo genetic modification of stem cells. For example, melatonin increases survival of MSCs and accelerates kidney recovery from acute kidney injury [8].…”

The Regenerative Medicine Laboratory: Facilitating Stem Cell Therapy for Equine Disease

“…Examples of these efforts include the use specific bioscaffolds which allow spatial and physical cell manipulation, increase cell survival and promote integration with the host. Furthermore, culture strategies have been evaluated to improve MSC viability by exposing cells to hypoxic conditions [10], specific growth factors [11,12], or a variety of biological agents [13].…”