14S,21R-dihydroxy-docosahexaenoic Acid Treatment Enhances Mesenchymal Stem Cell Amelioration of Renal Ischemia/Reperfusion Injury

Tian, Haibin; Lü, Yan; Shah, Sunish; Wang, Quansheng; Hong, Song

doi:10.1089/scd.2011.0220

Cited by 37 publications

(49 citation statements)

References 68 publications

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“…The antimicrobial activities of these essential fatty acids were also described in some plant species 21,22 but, to the best of our knowledge, no report was published regarding such an aspect in A. longa and B. dioïca. Consequently, the following section was designed to investigate the antibacterial activity of the lipid fraction of both species.…”

Section: Fatty Acids Pro Lementioning

confidence: 96%

Fatty Acids Composition and Antibacterial Activity of Aristolochia longa L. and Bryonia dioïca Jacq. Growing Wild in Tunisia

Dhouioui

Boulila²,

Jemli³

et al. 2016

J. Oleo Sci.

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International audienceThe composition of the fatty acids of the roots and aerial parts of Aritolochia longa (Aristolacheae) and Bryonia dioica (Cucurbutaceae) was analyzed by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS). The oils extracted from the aerial parts of both species were rich in polyunsaturated fatty acids with the essential linolenic and linoleic acids being the most prominent compounds. Oleic and linoleic acids were the majors fatty acids in the roots of both species. Whatever the plant part analyzed and the species, the saturated fatty acids were predominantly composed of palmitic and stearic acids. The antibacterial activity, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the lipid extracts were determined against a panel of five bacterial strains. The results showed that the sensitivity to the lipid extracts was different for the test bacterial strains, and the susceptibility of gram positive bacteria was found to be greater than gram negative bacteria. The antibacterial activity of the root lipid extracts was particularly important against Enterococcus feacium (CMI value of 125 mu g/mL; CMB values > 250 mu g/mL) and Streptococcus agalactiae (CMI value of 125 mu g/mL; CMB values 250 mu g/mL for A. longa roots). These results indicate that A. longa and B. dioica could be considered as good sources of essential fatty acids which can act as natural antibacterial agents

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Section: Fatty Acids Pro Lementioning

confidence: 96%

Fatty Acids Composition and Antibacterial Activity of Aristolochia longa L. and Bryonia dioïca Jacq. Growing Wild in Tunisia

Dhouioui

Boulila²,

Jemli³

et al. 2016

J. Oleo Sci.

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“…21,22 Systemically administered MSCs have been demonstrated to be able to travel to injured tissues and improve tissue regeneration such as heart, brain, renal, and skeletal tissues. [23][24][25][26][27] Preliminary clinical trials of MSCs for the treatment of myocardial ischemia and osteogenesis imperfecta have been performed and showed moderate beneficial effects. [28][29][30][31] The knowledge obtained from extensive studies of MSCs in cellular therapy could be applicable for tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

The Key Regulatory Roles of the PI3K/Akt Signaling Pathway in the Functionalities of Mesenchymal Stem Cells and Applications in Tissue Regeneration

Chen

Crawford

Chen

et al. 2013

Tissue Engineering Part B: Reviews

203

161

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Mesenchymal stem cells (MSCs) are multipotent cells that can differentiate into various cell types and have been widely used in tissue engineering application. In tissue engineering, a scaffold, MSCs and growth factors are used as essential components and their interactions have been regarded to be important for regeneration of tissues. A critical problem for MSCs in tissue engineering is their low survival ability and functionality. Most MSCs are going to be apoptotic after transplantation. Therefore, increasing MSC survival ability and functionalities is the key for potential applications of MSCs. Several approaches have been studied to increase MSC tissue forming capacity including application of growth factors, overexpression of stem cell regulatory genes, and improvement of biomaterials for scaffolds. The effects of these approaches on MSCs have been associated with activation of the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway. The pathway plays central regulatory roles in MSC survival, proliferation, migration, angiogenesis, cytokine production, and differentiation. In this review, we summarize and discuss the literatures related to the roles of the PI3K/Akt pathway in the functionalities of MSCs and the involvement of the pathway in biomaterials-increased MSC functionalities. Biomaterials have been modified in their properties and surface structure and loaded with growth factors to increase MSC functionalities. Several studies demonstrated that the biomaterials-increased MSC functionalities are mediated by the activation of the PI3K/Akt pathway. MSCs are going to be apoptotic after transplantation. Therefore, increasing MSC survival ability and functionalities is the key for potential applications of MSCs. Several approaches have been studied to increase MSC tissue forming capacity including application of growth factors, overexpression of stem cell regulatory genes, and improvement of biomaterials for scaffolds. The effects of these approaches on MSCs have been associated with activation of the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway. The pathway plays central regulatory roles in MSC survival, proliferation, migration, angiogenesis, cytokine production, and differentiation. In this review, we summarize and discuss the literatures related to the roles of the PI3K/Akt pathway in the functionalities of MSCs and the involvement of the pathway in biomaterials-increased MSC functionalities. Biomaterials have been modified in their properties and surface structure and loaded with growth factors to increase MSC functionalities. Several studies demonstrated that the biomaterials-increased MSC functionalities are mediated by the activation of the PI3K/Akt pathway.

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“…Accumulating evidence has demonstrated that the infusion of MSCs results in anti-inflammatory and anti-apoptotic effects in animal models of I/R injury [8,10,21,24,40]. Our research indicates that MSCs administration at the time of reperfusion could provide a degree of protection against AKI.…”

Section: Discussionmentioning

confidence: 55%

“…To address this problem, a number of studies aimed at promoting the retention and survival of MSCs to increase the number of cells successfully engrafting in the injured kidney have been published. The main strategy utilized has involved the alteration of ‘seeds' meaning donor cells by preconditioning or genetic modiﬁcation of MSCs [24,25,26]. However, these techniques do not always provide sufficient effects for the improvement of the cell survival, and further exploration is required for clinical application due to the complexity of these procedures.…”

Section: Discussionmentioning

confidence: 99%

Atorvastatin Improves Survival of Implanted Stem Cells in a Rat Model of Renal Ischemia-Reperfusion Injury

et al. 2014

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Aims: To investigate the impacts of combinatorial atorvastatin (Ator) perioperative administration and mesenchymal stem cell (MSC) implantation on therapeutic effects in the rat experimental acute kidney injury. Methods: The model of renal ischemia-reperfusion (I/R) injury was induced by the release of bilateral renal pedicle clamps following 45 min of occlusion. Immediately after reperfusion, CM-Dil-labeled MSCs (1 × 10⁶ cells) or vehicles only were administered through the carotid artery of the animals pretreated with or without Ator. Results: The combined treatment with Ator and MSCs (Ator+MSCs) markedly reduced the elevated levels of serum creatinine and blood urea nitrogen, as well as the severity of renal damage 24 h after I/R injury. In addition, we also observed inhibition of renal tubular cell apoptosis and promotion of proliferation in the Ator+MSCs group compared with the other groups. Consistent with the improvement in renal function and morphology, Ator pretreatment significantly ameliorated oxidative stress, inhibited inflammation response, and increased the viability of implanted MSCs. With regard to the further mechanism, we found that the expression of Toll-like receptor 4 (TLR4) and high-mobility group box 1, potential mediators of innate immunity, was significantly decreased in the Ator-treated groups. Conclusion: Ator treatment may protect the kidney undergoing I/R injury through suppression of TLR4 signaling, creating a better environment for the survival of grafted MSCs. The extra benefit of the Ator+MSCs combined therapy may result from the Ator-mediated inhibition of oxidative stress and inflammation in the ischemic kidney.

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14S,21R-dihydroxy-docosahexaenoic Acid Treatment Enhances Mesenchymal Stem Cell Amelioration of Renal Ischemia/Reperfusion Injury

Cited by 37 publications

References 68 publications

Fatty Acids Composition and Antibacterial Activity of <i>Aristolochia longa</i> L. and <i>Bryonia dioïca</i> Jacq. Growing Wild in Tunisia

Fatty Acids Composition and Antibacterial Activity of <i>Aristolochia longa</i> L. and <i>Bryonia dioïca</i> Jacq. Growing Wild in Tunisia

The Key Regulatory Roles of the PI3K/Akt Signaling Pathway in the Functionalities of Mesenchymal Stem Cells and Applications in Tissue Regeneration

Atorvastatin Improves Survival of Implanted Stem Cells in a Rat Model of Renal Ischemia-Reperfusion Injury

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