Ex vivo glucocorticoid‐induced secreted proteome approach for discovery of glucocorticoid‐responsive proteins in human serum

Inder, Warrick J.; Mohamed, Ahmed; Keshvari, Sahar; Barclay, Johanna L.; Ruelcke, Jayde E.; Stoll, Thomas; Nolan, Brendan J; Cesana-Nigro, Nicole; Hill, Michelle M.

doi:10.1002/prca.202000078

Proteomics Clinical Apps

2021

DOI: 10.1002/prca.202000078

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Ex vivo glucocorticoid‐induced secreted proteome approach for discovery of glucocorticoid‐responsive proteins in human serum

Warrick J. Inder

Ahmed Mohamed

Sahar Keshvari

et al.

Abstract: contributed equally to this study.

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Cited by 2 publications

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“…Inder et al searched for glucocorticoid-responsive serum proteinsreleased by PBMCs ex vivo exposed with 100 ng/ml of dexamethasone (DEX) for 4 and 24 h duration[39]. PBMCs were isolated by ™Ficoll-Paque and ex vivo treated with DEX.…”

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Human peripheral blood mononuclear cells: A review of recent proteomic applications

et al. 2022

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Human peripheral blood mononuclear cells (PBMCs) represent a sentinel blood sample which reacts to different pathophysiological stimuli in the form of immunological responses/immunophenotypic changes. The study of molecular content of PBMCs can provide better understanding of immune processes giving the possibility of monitoring the health conditions of the host organism. Proteomic analysis of PBMCs can achieve mentioned goal as important immune-related biomarkers are easily accessible for analysis. PBMCs have been gaining attention in different research areas including preclinical or clinical investigations. In this review, recent applications of proteomic analysis of PBMCs are described and discussed. Approaches are divided based on different proteomic workflows such as in-gel, in-solution and on-filter modes. The effect of various diseases such as autoimmune, cancer, neurodegenerative, viral, metabolic, and various immune stimulations such as radiation, vaccine, corticosteroids over PBMCs proteome, are described with emphasis on promising protein biomarker candidates.

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Human peripheral blood mononuclear cells: A review of recent proteomic applications

et al. 2022

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Current Challenges and Future Directions in the Assessment of Glucocorticoid Status

Clarke,

Eng,

Comninos

et al. 2024

Endocrine Reviews

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Glucocorticoid hormones (GC) are secreted in a circadian and ultradian rhythm and play a critical role in maintaining physiological homeostasis, with both excess and insufficient GC associated with adverse effects on health. Current assessment of GC status is primarily clinical, often in conjunction with serum cortisol values, which may be stimulated or suppressed depending on the GC disturbance being assessed. In the setting of extreme perturbations in cortisol levels i.e. markedly low or high levels, symptoms and signs of GC dysfunction may be overt. However, when disturbances in cortisol GC status values are less extreme, such as when assessing optimization of a GC replacement regimen, signs and symptoms can be more subtle or non-specific. Current tools for assessing GC status, are best suited to identifying profound disturbances but may lack sensitivity for confirming optimal GC status. Moreover, single cortisol values do not necessarily reflect an individual’s GC status, as they are subject to inter- and intra-individual variation, do not take into account the pulsatile nature of cortisol secretion, variation in binding proteins, or local tissue concentrations as dictated by 11βeta-hydroxysteroid dehydrogenase (11β-HSD) activity, as well as GC receptor sensitivity. In the present review, we evaluate possible alternative methods for the assessment of GC status that do not solely rely on measurement of circulating cortisol levels. We discuss the potential of changes in metabolomic profiles, miRNA, gene expression, epigenetic, and other novel biomarkers such as GDF-15 and osteocalcin, that could in future aid in the objective classification of GC status.

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Ex vivo glucocorticoid‐induced secreted proteome approach for discovery of glucocorticoid‐responsive proteins in human serum

Abstract: contributed equally to this study.

Cited by 2 publications

References 39 publications

Human peripheral blood mononuclear cells: A review of recent proteomic applications

Human peripheral blood mononuclear cells: A review of recent proteomic applications

Current Challenges and Future Directions in the Assessment of Glucocorticoid Status

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