Human peripheral blood mononuclear cells: A review of recent proteomic applications

Abstract

Human peripheral blood mononuclear cells (PBMCs) represent a sentinel blood sample which reacts to different pathophysiological stimuli in the form of immunological responses/immunophenotypic changes. The study of molecular content of PBMCs can provide better understanding of immune processes giving the possibility of monitoring the health conditions of the host organism. Proteomic analysis of PBMCs can achieve mentioned goal as important immune-related biomarkers are easily accessible for analysis. PBMCs have been gaining attention in different research areas including preclinical or clinical investigations. In this review, recent applications of proteomic analysis of PBMCs are described and discussed. Approaches are divided based on different proteomic workflows such as in-gel, in-solution and on-filter modes. The effect of various diseases such as autoimmune, cancer, neurodegenerative, viral, metabolic, and various immune stimulations such as radiation, vaccine, corticosteroids over PBMCs proteome, are described with emphasis on promising protein biomarker candidates.

The Effects of a Multi-Ingredient Supplement Containing Wasabia Japonica Extract, Theacrine, and Copper (I) Niacin Chelate on Peripheral Blood Mononuclear Cell DNA Methylation, Transcriptomics, and Sirtuin Activity

“…PBMCs contain lymphocytes (T cells, B cells, and NK cells), monocytes, and dendritic cells [23]. T cells have long half-lives (i.e., 1-8 years) [24], whereas B cells and monocytes have relatively shorter half-lives (i.e., days to weeks) [25,26]. Given that increased helicase activity coincides with cell cycle progression [27], future research is needed to examine how some of the shorter-lived PBMCs (e.g., B cells and monocytes) are affected by NAD3 supplementation.…”

Genome-Wide Association Study Reveals CLEC7A and PROM1 as Potential Regulators of Paracoccidioides brasiliensis-Induction of Cytokine Production in Peripheral Blood Mononuclear Cells

“…In PBMCs, human monocytes react to pathophysiological changes in human body; thus, these cells are suitable samples when searching for biomarkers as cytokines and genes/molecules involved in their genetic control [15]. Some studies have shown that the cytokines IFNγ and TNF are important to control the growth of P. brasiliensis in human monocytes and macrophages [16][17][18].…”

Shared and Unique Disease Pathways in Amyotrophic Lateral Sclerosis and Parkinson’s Disease Unveiled in Peripheral Blood Mononuclear Cells

“…Moreover, compared to plasma and serum, which are also easy to collect, PBMCs offer the possibility to detect and quantify low-abundance proteins, which are usually neglected due to the wide dynamic range of protein concentrations in blood-derived fluids. Eventually, several recent studies revealed that PBMCs (i.e., T and B lymphocytes, natural killer cells, and monocytes) nicely recapitulate the conditions of the surrounding tissues. , We used 2-DE to compare the proteome of T-lymphocytes from PD patients with that of control subjects, which led us to the identification of a panel of 17 proteins that performed well as a classifier for PD . The main limitation of this protein panel was that PD patients were compared with healthy subjects.…”