Ex Vivo Expansion of Haematopoietic Stem Cells to Improve Engraftment in Stem Cell Transplantation

Ko, Kap-Hyoun; Nordon, Robert E.; O’Brien, Tracey; Symonds, Geoff; Dolnikov, Alla

doi:10.1007/978-1-61779-182-6_17

Cited by 9 publications

(5 citation statements)

References 20 publications

(20 reference statements)

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“…One of the major weaknesses of this trial was its reliance on the expansion of just a fraction (40-60%) of a single CB unit. Given that the success of stem cell transplantation is largely dictated by the cell dose [150], there was little chance for hematological improvements. Nonetheless, this early trial, and others, demonstrated the safety of such an approach [151,152 ].…”

Section: Translation Of Ex Vivo Expanded Cb Progenitors To the Clinicmentioning

confidence: 99%

Advances in umbilical cord blood stem cell expansion and clinical translation

Pineault

Abu‐Khader

2015

Experimental Hematology

106

101

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Section: Translation Of Ex Vivo Expanded Cb Progenitors To the Clinicmentioning

confidence: 99%

Advances in umbilical cord blood stem cell expansion and clinical translation

Pineault

Abu‐Khader

2015

Experimental Hematology

106

101

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“…Furthermore, BMSCs transplantation alleviated clinical signs, infiltration of inflammatory cells and demyelination of EAE mice, and down-regulated the expression levels of pro-inflammatory factors TNF-α, IL-1β and IFN-γ, while increasing the expression level of anti-inflammatory factors IL-10 and TGF-β. Although stem cell transplantation is advanced, the success rate of stem cell transplantation is relatively low [ 44 ]. Some studies have evaluated the effect of stem cell transplantation on the clinical treatment of MS [ 45 – 47 ].…”

Section: Discussionmentioning

confidence: 99%

BMSCs differentiated into neurons, astrocytes and oligodendrocytes alleviated the inflammation and demyelination of EAE mice models

Guo-yi

Kong³

et al. 2021

PLoS ONE

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Multiple sclerosis (MS) is a complex, progressive neuroinflammatory disease associated with autoimmunity. Currently, effective therapeutic strategy was poorly found in MS. Experimental autoimmune encephalomyelitis (EAE) is widely used to study the pathogenesis of MS. Cumulative research have shown that bone marrow mesenchymal stem Cells (BMSCs) transplantation could treat EAE animal models, but the mechanism was divergent. Here, we systematically evaluated whether BMSCs can differentiate into neurons, astrocytes and oligodendrocytes to alleviate the symptoms of EAE mice. We used Immunofluorescence staining to detect MAP-2, GFAP, and MBP to evaluate whether BMSCs can differentiate into neurons, astrocytes and oligodendrocytes. The effect of BMSCs transplantation on inflammatory infiltration and demyelination in EAE mice were detected by Hematoxylin-Eosin (H&E) and Luxol Fast Blue (LFB) staining, respectively. Inflammatory factors expression was detected by ELISA and RT-qPCR, respectively. Our results showed that BMSCs could be induced to differentiate into neuron cells, astrocytes and oligodendrocyte in vivo and in vitro, and BMSCs transplanted in EAE mice were easier to differentiate than normal mice. Moreover, transplanted BMSCs reduced neurological function scores and disease incidence of EAE mice. BMSCs transplantation alleviated the inflammation and demyelination of EAE mice. Finally, we found that BMSCs transplantation down-regulated the levels of pro-inflammatory factors TNF-α, IL-1β and IFN-γ, and up-regulated the levels of anti-inflammatory factors IL-10 and TGF-β. In conclusion, this study found that BMSCs could alleviate the inflammatory response and demyelination in EAE mice, which may be achieved by the differentiation of BMSCs into neurons, astrocytes and oligodendrocytes in EAE mice.

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“…Although stem cell transplantation is advanced, the success rate of stem cell transplantation is relatively low [44]. Some studies have evaluated the effect of stem cell transplantation on the clinical treatment of MS [45][46][47].…”

Section: Discussionmentioning

confidence: 99%

BMSCs differentiated into neurons, astrocytes and oligodendrocytes alleviated the inflammation and demyelination of EAE mice models

Geng

Liu

et al. 2020

Preprint

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Multiple sclerosis (MS) is a complex, progressive neuroinflammatory disease associated with autoimmunity. Currently, effective therapeutic strategy was poorly found in MS. Experimental autoimmune encephalomyelitis (EAE) is widely used to study the pathogenesis of MS. Previous studies have shown that bone marrow mesenchymal stem Cells (BMSCs) transplantation could treat EAE animal models, but the mechanism was divergent. Here, we systematically evaluated whether BMSCs can differentiate into neurons, astrocytes and oligodendrocytes to alleviate the symptoms of EAE mice. We used Immunofluorescence staining to detect MAP-2 neurons marker, GFAP astrocytes marker, and MBP oligodendrocytes marker expression to evaluate whether BMSCs can differentiate. The effect of BMSCs transplantation on inflammatory cell invasion and demyelination in EAE mice were detected by Hematoxylin-Eosin (H&E) and Luxol Fast Blue (LFB) staining. Inflammatory factors expression was detected by ELISA and RT-qPCR. Our results showed that BMSCs could be induced to differentiate into neuron cells, astrocytes and oligodendrocyte in vivo and in vitro. In addition, BMSCs transplant improved the survival rate and weight, and reduced neurological function scores and disease incidence of EAE mice. Moreover, BMSCs transplant alleviated the inflammation and demyelination of EAE mice. Finally, we found that BMSCs transplantation down-regulated the expression levels of pro-inflammatory factors TNF-α, IL-1β and IFN-γ, and up-regulated the expression levels of anti-inflammatory factors IL-10 and TGF-β. In conclusion, this study found that BMSCs could alleviate the inflammatory response and demyelination in EAE mice, which may be achieved by the differentiation of BMSCs into neurons, astrocytes and oligodendrocytes in EAE mice.

show abstract

Ex Vivo Expansion of Haematopoietic Stem Cells to Improve Engraftment in Stem Cell Transplantation

Cited by 9 publications

References 20 publications

Advances in umbilical cord blood stem cell expansion and clinical translation

Advances in umbilical cord blood stem cell expansion and clinical translation

BMSCs differentiated into neurons, astrocytes and oligodendrocytes alleviated the inflammation and demyelination of EAE mice models

BMSCs differentiated into neurons, astrocytes and oligodendrocytes alleviated the inflammation and demyelination of EAE mice models

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