BMSCs differentiated into neurons, astrocytes and oligodendrocytes alleviated the inflammation and demyelination of EAE mice models

Abstract

Multiple sclerosis (MS) is a complex, progressive neuroinflammatory disease associated with autoimmunity. Currently, effective therapeutic strategy was poorly found in MS. Experimental autoimmune encephalomyelitis (EAE) is widely used to study the pathogenesis of MS. Cumulative research have shown that bone marrow mesenchymal stem Cells (BMSCs) transplantation could treat EAE animal models, but the mechanism was divergent. Here, we systematically evaluated whether BMSCs can differentiate into neurons, astrocytes and oligodendrocytes to alleviate the symptoms of EAE mice. We used Immunofluorescence staining to detect MAP-2, GFAP, and MBP to evaluate whether BMSCs can differentiate into neurons, astrocytes and oligodendrocytes. The effect of BMSCs transplantation on inflammatory infiltration and demyelination in EAE mice were detected by Hematoxylin-Eosin (H&E) and Luxol Fast Blue (LFB) staining, respectively. Inflammatory factors expression was detected by ELISA and RT-qPCR, respectively. Our results showed that BMSCs could be induced to differentiate into neuron cells, astrocytes and oligodendrocyte in vivo and in vitro, and BMSCs transplanted in EAE mice were easier to differentiate than normal mice. Moreover, transplanted BMSCs reduced neurological function scores and disease incidence of EAE mice. BMSCs transplantation alleviated the inflammation and demyelination of EAE mice. Finally, we found that BMSCs transplantation down-regulated the levels of pro-inflammatory factors TNF-α, IL-1β and IFN-γ, and up-regulated the levels of anti-inflammatory factors IL-10 and TGF-β. In conclusion, this study found that BMSCs could alleviate the inflammatory response and demyelination in EAE mice, which may be achieved by the differentiation of BMSCs into neurons, astrocytes and oligodendrocytes in EAE mice.

Laser-activated autologous adipose tissue-derived stromal vascular fraction restores spinal cord architecture and function in multiple sclerosis cat model

“…[ 92 ] recorded that MSCs increase the migration of oligodendrocyte progenitors from surrounding niches by various trophic signals from the grafted MSCs. [ 93 ] provide evidence on the direct differentiation of MSCs into oligodendrocytes by transplantation of GFP-labeled BMSCs into EAE mice model. Secondly, MSCs can increase differentiated oligodendrocyte progenitors into mature oligodendrocyte and myelin production as observed by increased immune expression of Olig2 and MPB.…”

Tauroursodeoxycholic Acid Inhibited Apoptosis and Oxidative Stress in H2O2-Induced BMSC Death via Modulating the Nrf-2 Signaling Pathway: the Therapeutic Implications in a Rat Model of Spinal Cord Injury

“…Transplantation of cells, especially BMSCs, has emerged as a potential treatment option for SCI [ 12 , 28 , 29 ]. Numerous studies have shown that BMSCs can differentiate into neuron-like cells, promoting neuronal regeneration and angiogenesis while inhibiting inflammation, making them promising candidates for SCI therapy [ 30 – 33 ]. However, BMSC transplantation faces challenges such as low survival rates and limited differentiation potential, primarily due to the OS microenvironment induced by secondary SCI, which significantly hampers treatment effectiveness.…”

Human bone marrow mesenchymal stem cell-driven LncRNA PTCSC3 upregulation within lung adenocarcinoma cells reduces erlotinib resistance by mitigating Wnt/β-Catenin pathway

“…Recent research has highlighted the pivotal role of MSCs on drug resistance of tumor cells, demonstrating that MSCs revolutionized the treatment of neoplastic diseases in the future [22,23]. hBMSCs, a subgroup of bone marrow stroma cells, have diverse differentiation potentials including forming bone, cartilage, fat, nerve and myoblasts [24]. They play a critical role in Am J Cancer Res 2024;14(5):2439-2452 providing mechanical support to hematopoietic stem cells (HSCs) in bone marrow and secreting growth factors (IL-6, IL-11, LIF, etc.)…”