Ex vivo development of functional human lymph node and bronchus-associated lymphoid tissue

Tirouvanziam, Rabindra; Khazaal, I; N'Sondé, Victoire; Peyrat, Marie-Alix; Lim, Annick; Bentzmann, Sophie de; Fournié, Jean Jacques; Bonneville, Marc; Péault, Bruno

doi:10.1182/blood.v99.7.2483

Cited by 12 publications

(7 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Long-lasting antigen stimuli promote the hyperplasia of BALT, which may develop into pulmonary mucosa-associated lymphoma (12, 13). Chronic inflammatory diseases could evoke oligo- or monoclonal cell proliferation in the mucosal lymphoid tissue, resulting in the development of lymphoma (14).…”

Section: Discussionmentioning

confidence: 99%

Primary Endobronchial Marginal Zone B-Cell Lymphoma of Bronchus-Associated Lymphoid Tissue: CT Findings in 7 Patients

et al. 2013

View full text Add to dashboard Cite

ObjectiveTo investigate CT and 18F-flurodeoxyglucose (18F-FDG) positron-emission tomography/CT findings of primary endobronchial marginal zone B-cell lymphoma of the bronchus-associated lymphoid tissue (BALT).Materials and MethodsFrom June 2006 through April 2012, seven patients (six female, one male; age range, 21-61 years; mean age, 49 years) were examined who were pathologically diagnosed with the primary endobronchial marginal zone B-cell lymphoma of BALT. We evaluated the locations and characteristics of the lesions on CT and 18F-FDG-PET/CT scans. The lesions were classified into the following three patterns: 1) solitary intraluminal nodule; 2) several tiny nodular protrusions; and 3) diffuse wall thickening.ResultsA solitary intraluminal nodule was observed in four patients (57.1%), several tiny nodular protrusion in two patients (28.6%), and diffuse wall thickening in one patient (14.3%). The lesions were categorized into 3 major locations: confined to the trachea (n = 3), confined to the lobar bronchus (n = 2), and diffuse involvement of the trachea and both main bronchi (n = 2). All lesions demonstrated homogeneous iso-attenuation as compared with muscle on pre- and post-enhancement scans. Secondary findings in the lungs (n = 3; 42.9%) included postobstructive lobar atelectasis (n = 1), air trapping (n = 1), and pneumonia (n = 1). On 18F-FDG-PET/CT (n = 5), 4 lesions showed homogeneous uptake with maximum standardized uptake values (mSUV), ranging 2.3-5.7 (mean mSUV: 3.3). One lesion showed little FDG uptake.ConclusionPrimary endobronchial marginal zone B-cell lymphoma of the BALT manifests as three distinct patterns on CT, with the solitary intraluminal nodule presenting as the main pattern. Most lesions demonstrate homogeneous but weak FDG uptake on 18F-FDG-PET/CT.

show abstract

Section: Discussionmentioning

confidence: 99%

Primary Endobronchial Marginal Zone B-Cell Lymphoma of Bronchus-Associated Lymphoid Tissue: CT Findings in 7 Patients

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Further evidence to support the interrelationship between LNs and BALT formation was illustrated following the transplantation of human fetal bronchial tissue to SCID mice; only when the peripheral LNs were cotransplanted did the mice develop BALT. 85 Further studies will be required to delineate the origin of the cells targeted by Tregs and the molecular processes that mediate this inhibition. Curiously, immunophenotyping of bronchial biopsy specimen from human infants has shown that Foxp3 + CD4 + Tregs reside primarily within these follicles.…”

Section: Contribution Of Regulatory T Cells To Ibalt Formationmentioning

confidence: 99%

Regulation of inducible BALT formation and contribution to immunity and pathology

2010

View full text Add to dashboard Cite

Inducible bronchus-associated lymphoid tissue (iBALT) is an organized tertiary lymphoid structure that is not pre-programmed but develops in response to infection or under chronic inflammatory conditions. Emerging research has shown that iBALT provides a niche for T-cell priming and B-cell education to assist in the clearance of infectious agents, highlighting the prospect that iBALT may be engineered and harnessed to enhance protective immunity against respiratory pathogens. Although iBALT formation is associated with several canonical factors of secondary lymphoid organogenesis such as lymphotoxin-α and the homeostatic chemokines, CXCL13, CCL19, and CCL21, these cytokines are not mandatory for its formation, even though they influence its organization and function. Similarly, lymphoid tissue-inducer cells are not a requisite of iBALT formation. In contrast, dendritic cells are emerging as pivotal players required to form and sustain the presence of iBALT. Regulatory T cells appear to be able to attenuate the development of iBALT, although the underlying mechanisms remain ill-defined. In this review, we discuss facets unique to iBALT induction, the cellular subsets, and molecular cues that govern this process, and the contribution of this ectopic structure toward the generation of immune responses in the pulmonary compartment.

show abstract

“…The transplantation of human fetal bronchial tissue into severe combined immunodeficient (SCID) mice with and without other tissues, including bone marrow, thymus, peripheral lymph nodes, and liver, has led to interesting observations (43). Only co‐transplantation of peripheral lymph nodes resulted in BALT, and these invsetigators suggested that peripheral lymph nodes are necessary for the lymphocytic population of BALT follicles, which in their model were able to mount a vigorous T‐cell response to both pseudomonas and chemical challenge (43).…”

Section: Ontogenymentioning

confidence: 99%

Bronchus‐ and nasal‐associated lymphoid tissues

Bienenstock

McDermott

2005

Immunological Reviews

172

142

View full text Add to dashboard Cite

The bronchus-associated lymphoid tissue (BALT) and the nasal-associated lymphoid tissue (NALT) constitute organized lymphoid aggregates that are capable of T- and B-cell responses to inhaled antigens. BALT, located mostly at bifurcations of the bronchus in animals and humans, is present in the fetus and develops rapidly following birth, especially in the presence of antigens. Humoral immune responses elicited by BALT are primarily immunoglobulin A secretion both locally and by BALT-derived B cells that have trafficked to distant mucosal sites. Similarly located T-cell responses have been noted. On the basis of these findings, the BALT can be thought of as functionally analogous to mucosal lymphoid aggregates in the intestine and is deemed a member of the common mucosal immunologic system. NALT has been described principally in the rodent nasal passage as two separate lymphoid aggregates. It develops after birth, likely in response to antigen, and B- and T-cell responses parallel those that occur in BALT. It is not known whether NALT cells traffic to distant mucosal sites, although mucosal responses have been detected after nasal immunization. NALT appears from many studies to be a functionally distinct lymphoid aggregate when compared with BALT and Peyer's patches. It may exist, however, in humans as a diffuse collection of isolated lymphoid follicles.

show abstract

Ex vivo development of functional human lymph node and bronchus-associated lymphoid tissue

Cited by 12 publications

References 25 publications

Primary Endobronchial Marginal Zone B-Cell Lymphoma of Bronchus-Associated Lymphoid Tissue: CT Findings in 7 Patients

Primary Endobronchial Marginal Zone B-Cell Lymphoma of Bronchus-Associated Lymphoid Tissue: CT Findings in 7 Patients

Regulation of inducible BALT formation and contribution to immunity and pathology

Bronchus‐ and nasal‐associated lymphoid tissues

Contact Info

Product

Resources

About