Evolutionary relationships within Aspergillus section Flavi based on sequences of the intergenic transcribed spacer regions and the 5.8S rRNA gene.

Rigó, Krisztina; Varga, János; Töth, Beáta; Téren, József; Mesterházy, Ákos; Kozakiewicz, Z.

doi:10.2323/jgam.48.9

Cited by 47 publications

(33 citation statements)

References 36 publications

(37 reference statements)

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“…S4). These results supported the difficulty in the differentiation of A. oryzae and A. sojae from the closely related aflatoxin-producing species by analysis based on only a single gene locus (14,16).…”

supporting

confidence: 63%

“…Although clear differences were found in 0.5% of the total genome sequence (equivalent to approx. 200 kbp) between a non-aflatoxinproducer, A. oryzae, and an aflatoxin-producer, A. flavus (17), their 18S rRNA, 26S rRNA, and ITS regions are almost identical (14,16). A similar example has been reported in the molecular identification of A. sojae, A. parasiticus, and A. toxicarius (14,16).…”

mentioning

confidence: 54%

“…On the other hand, several species belonging to this section, such as A. flavus, A. parasiticus, and A. toxicarius, are known to produce aflatoxin, a toxic and carcinogenic mycotoxin (6,16). In recent years, molecular phylogenetic approaches on the basis of small (5.8S and 18S) and large (28S) subunits of the rRNA gene and their internal transcribed spacer (ITS) region sequences have been applied for classification of the genus Aspergillus section Flavi in the same manner as the classification of prokaryotes (16). However, accurate identification of the closely related Aspergillus species within the section Flavi using these phylogenetic markers is still difficult because the fungal rRNA gene and ITS region sequences are highly conserved as compared with prokaryotes.…”

mentioning

confidence: 99%

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Evaluation of the Aflatoxin Biosynthetic Genes for Identification of the Aspergillus Section Flavi

et al. 2011

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Several fungi in the Aspergillus section Flavi have been widely used for fermentative food production, while some related species in the section are known to produce mycotoxin(s) that causes mycotic diseases. Common evolutionary markers, such as rRNA gene sequences and their internal transcribed spacers, cannot differentiate these non-aflatoxinproducing species from aflatoxin producers. Multilocus sequence analysis (MLSA) based on four aflatoxin biosynthetic genes encoding aflR, aflT, norA, and vbs, which are more variable nucleotide sequences than rRNA genes, can distinguish safe koji molds, A. oryzae and A. sojae, from aflatoxin-producing strains, A. flavus, A. toxicarius and A. parasiticus.

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supporting

confidence: 63%

mentioning

confidence: 54%

mentioning

confidence: 99%

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Evaluation of the Aflatoxin Biosynthetic Genes for Identification of the Aspergillus Section Flavi

et al. 2011

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“…This complex seems to comprise distinct clades (Rigo et al, 2002). The three main clades (P. alliaceus, A. flavus and A. tamarii) could also be distinguished based on colony colour and their ubiquinone system.…”

Section: T Hedayati and Others 1680mentioning

confidence: 92%

“…In addition, A. pseudotamarii and A. bombycis were found to be closely related to A. caelatus and A. nomius, respectively (Table 1). Rigo et al (2002) suggested that A. zonatus and A. clavatoflavus should be excluded from Aspergillus flavus complex, a suggestion previously made by Kozakiewicz (1989), based on scanning electron microscopic studies. Recently, Frisvad et al (2005) found that A. toxicarius resembles A. parasiticus but differs in at least three sequence differences in the ITS regions, as compared to four strains of A. parasiticus.…”

Section: T Hedayati and Others 1680mentioning

confidence: 99%

Aspergillus flavus: human pathogen, allergen and mycotoxin producer

et al. 2007

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Aspergillus infections have grown in importance in the last years. However, most of the studies have focused on Aspergillus fumigatus, the most prevalent species in the genus. In certain locales and hospitals, Aspergillus flavus is more common in air than A. fumigatus, for unclear reasons. After A. fumigatus, A. flavus is the second leading cause of invasive aspergillosis and it is the most common cause of superficial infection. Experimental invasive infections in mice show A. flavus to be 100-fold more virulent than A. fumigatus in terms of inoculum required. Particularly common clinical syndromes associated with A. flavus include chronic granulomatous sinusitis, keratitis, cutaneous aspergillosis, wound infections and osteomyelitis following trauma and inoculation. Outbreaks associated with A. flavus appear to be associated with single or closely related strains, in contrast to those associated with A. fumigatus. In addition, A. flavus produces aflatoxins, the most toxic and potent hepatocarcinogenic natural compounds ever characterized. Accurate species identification within Aspergillus flavus complex remains difficult due to overlapping morphological and biochemical characteristics, and much taxonomic and population genetics work is necessary to better understand the species and related species. The flavus complex currently includes 23 species or varieties, including two sexual species, Petromyces alliaceus and P. albertensis. The genome of the highly related Aspergillus oryzae is completed and available; that of A. flavus in the final stages of annotation. Our understanding of A. flavus lags far behind that of A. fumigatus. Studies of the genomics, taxonomy, population genetics, pathogenicity, allergenicity and antifungal susceptibility of A. flavus are all required.

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Phylogeny and molecular diagnosis of mycotoxigenic fungi

Seifert

Lévesque

2004

Molecular Diversity and PCR-detection of Toxigenic Fusarium Species and Ochratoxigenic Fungi

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Evolutionary relationships within Aspergillus section Flavi based on sequences of the intergenic transcribed spacer regions and the 5.8S rRNA gene.

Cited by 47 publications

References 36 publications

Evaluation of the Aflatoxin Biosynthetic Genes for Identification of the Aspergillus Section Flavi

Evaluation of the Aflatoxin Biosynthetic Genes for Identification of the Aspergillus Section Flavi

Aspergillus flavus: human pathogen, allergen and mycotoxin producer

Phylogeny and molecular diagnosis of mycotoxigenic fungi

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