Evolutionary history of the Pasque-flowers (Pulsatilla, Ranunculaceae): Molecular phylogenetics, systematics and rDNA evolution

Sramkó, Gábor; Laczkó, Levente; Volkova, Polina A.; Bateman, Richard M.; Mlinarec, Jelena

doi:10.1016/j.ympev.2019.02.015

Cited by 17 publications

(17 citation statements)

References 77 publications

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“…Global climate perturbations throughout the Quaternary period caused active migrations of Pulsatilla within Eurasia, followed by secondary polyploidy and increased polymorphism ( Sramko et al 2019 ). The ancestor of the modern P. patens s.lat.…”

Section: Discussionmentioning

confidence: 99%

“…The ancestor of the modern P. patens s.lat. populations inhabiting north-eastern European Russia most likely grew in the steppes of Eastern Eurasia ( Sramko et al 2019 ). During the Pleistocene, the ancestor of the modern P. patens s.lat., perhaps, together with the southern Siberian forest-steppe species, spread to the west and further north along the Ural Mountains ( Knyazev et al 2007 , Kulikov et al 2013 ).…”

Section: Discussionmentioning

confidence: 99%

“…Such a relationship of the complex of P. patens s.lat. and P. vernalis with erect flowers and less dissected leaf blades was found by Sramko et al (2019) , who suggested to include P. vernalis into Series Patentes . Our phylogenetic trees showed that P. vernalis is close to P. patens s.str.…”

Section: Discussionmentioning

confidence: 99%

“…(e.g. Hoot et al 1994 , 2012 ), comprises more than 30 taxa which, as a rule, form intricate species complexes with a high degree of morphological variability ( Stepanov 2014 ; Li et al 2019 ; Sramko et al 2019 ). The general classification, boundaries and the number of species and lower taxa have been revised several times, but no consensus has yet been reached (e.g.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, there are several questions regarding the identification and size of taxa within the P. patens s.lat. complex, despite several recent studies on molecular phylogenetic relationships in the genus ( Li et al 2019 ; Sramko et al 2019 ).…”

Section: Introductionmentioning

confidence: 99%

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Phylogenetic relationships and status of taxa of Pulsatilla uralensis and P. patens s.str. (Ranunculaceae) in north-eastern European Russia

Valuyskikh¹,

Teteryuk²,

Pylina³

et al. 2020

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We studied the allopatric complex Pulsatilla patens (L.) Mill. s.lat. (Ranunculaceae) in north-eastern European Russia and the Urals. In this region, there are two kinds of P. patens with different perianth colours in monochrome and polychrome populations. To clarify their taxonomic boundaries, we used the sequences of chloroplast DNA (rbcL and matK) and nuclear DNA (ITS2), in addition to morphological characteristics. The combination of three markers (rbcL+matK+ITS2) was found to be the most effective for phylogenetic resolution. The samples of two morphologically-different taxa P. uralensis and P. patents s.str. were shown to form a single clade on the phylogenetic tree. Based on the molecular phylogenetic analysis, we were not able to unequivocally prove the independent existence of P. uralensis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phylogenetic relationships and status of taxa of Pulsatilla uralensis and P. patens s.str. (Ranunculaceae) in north-eastern European Russia

Valuyskikh¹,

Teteryuk²,

Pylina³

et al. 2020

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The uneven distribution of refugial endemics across the European Alps suggests a threefold role of climate in speciation of refugial populations

Kadereit

2024

Alp Botany

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A little more than 10% of the vascular plant flora native to the European Alps is endemic to this area. It has long been noticed that the distribution of endemics across the Alps is very uneven. While most endemics are found along the southern edge of the Alps, with some also on its western, eastern, and northeastern edges, the northern edge of the Alps more or less between Lake Geneva in the west and Lake Traun in the east harbours almost no endemics. The distribution of endemics in the Alps has often been related to the location of glacial refugia. Accordingly, the virtual absence of endemics from the northern edge of the Alps has been explained with the unsuitability of climatic conditions for glacial survival of alpine plants there. After discussing evidence for the existence of glacial refugia for alpine species along the northern edge of the Alps and north of the Alps, I will examine how these refugia differed from refugia along the southern edge of the Alps. I conclude that the uneven distribution of endemics in the Alps is best explained by the different climate through time north and south of the Alps. These climatic differences affected the spatial structure and extent of refugia, the length of isolation of refugial populations, and selective conditions in refugia.

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Genotype-dependent mass somatic embryogenesis: a chance to recover extinct populations of Pulsatilla vulgaris Mill.

Żabicka

Żabicki

Słomka

et al. 2021

Plant Cell Tiss Organ Cult

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The paper presents a technique for micropropagation of endangered in Europe and extinct in Poland Pulsatilla vulgaris for ex situ conservation of the genetic resources. Genotype-dependent induction of somatic embryogenesis and rooting was revealed in series of two experiments (I and II) performed under the same experimental conditions. Shoot tips of seedlings were the best explants in both experiments and Murashige and Skoog (MS) medium supplemented with 0.25 or 0.5 mg L−1 BAP was suitable for induction of somatic embryos (SE) and adventitious shoots. Mass SE was obtained in experiment I after explants transfer on ½ MS (2% sucrose) + 0.45 mg L−1 B1 and extending culture to 2–3 months without passages. Rooting of adventitious shoots was a critical point. Out of seven rooting media used in experiment I, only two, ½ MS hormone free (2% sucrose) + 0.45 mg L−1 B1 or MS + 5 mg L−1 NAA + 3.76 mg L−1 B2 resulted in altogether 36.4% rooted shoots. In experiment II, somatic embryogenesis, rooting and acclimatization of adventitious shoots failed. Regenerated plantlets and seedlings converted from SE from experiment I were acclimatized to ex vitro conditions. Both genome size, determined by flow cytometry, and genetic diversity analyzed by ISSR markers, confirmed the compatibility of regenerants from experiment I with P. vulgaris initial seedlings and commercial cultivar. Regenerants obtained in experiment II differed genetically from the regenerants of experiment I and cultivar. Propagated in vitro tissues/organs (SE, adventitious shoots) of P. vulgaris could be a source of material for cryopreservation, artificial seed production and/or for acclimatization of regenerated plantlets and could be used for restoration of the extinct populations. Key Message The micropropagation technique via organogenesis and somatic embryogenesis of endangered in Europe pasqueflower was developed as a tool for species recovery. The critical point is that somatic embryogenesis is genotype-dependent, which affects the repeatability of the experiments and also imposes applying molecular techniques to confirm the genetic fidelity of the regenerants with the initial material and other genotypes.

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Evolutionary history of the Pasque-flowers (Pulsatilla, Ranunculaceae): Molecular phylogenetics, systematics and rDNA evolution

Cited by 17 publications

References 77 publications

Phylogenetic relationships and status of taxa of Pulsatilla uralensis and P. patens s.str. (Ranunculaceae) in north-eastern European Russia

Phylogenetic relationships and status of taxa of Pulsatilla uralensis and P. patens s.str. (Ranunculaceae) in north-eastern European Russia

The uneven distribution of refugial endemics across the European Alps suggests a threefold role of climate in speciation of refugial populations

Genotype-dependent mass somatic embryogenesis: a chance to recover extinct populations of Pulsatilla vulgaris Mill.

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