Phylogenetic relationships and status of taxa of Pulsatilla uralensis and P. patens s.str. (Ranunculaceae) in north-eastern European Russia

Valuyskikh, Olga E.; Teteryuk, L. V.; Pylina, Yana I.; Сушенцов, О. Е.; Martynenko, Nikita; Shadrin, Dmitry M.

doi:10.3897/phytokeys.162.53361

Cited by 3 publications

(1 citation statement)

References 17 publications

(27 reference statements)

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“…DNA markers are an effective method for analyzing phylogenetic relationships and evolutionary processes and the most commonly used DNA markers include ITS, matK, rpoB, rbcL, psbA-trnH, and others (Adolfo et al 2022; Kurian et al 2020;Li et al 2022). As single DNA markers cannot distinguish genetic differences in closely related species, combined sequences (such as ITS + trnL-trnF (Feng et al 2019), rbcL + matK + ITS2 (Valuyskikh et al 2020) have been extensively used. However, few studies have used DNA markers in the phylogenetic analysis of L. japonicus.…”

Section: Introductionmentioning

confidence: 99%

Relevance of genetic and active ingredient content differences in Leonurus japonicus Houtt from different origins

Han

Wen

Chen

et al. 2023

Preprint

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Leonurus japonicus Houtt. (Labiatae), a perennial herb, is used to treat cardiovascular, uterine, and gynecological diseases. In the present study, a phylogenetic tree was constructed based on the ITS + psbA-trnH + rbcL + rpoB concatenation sequence, and partial least squares-discriminant analysis (PLS-DA) was performed based on high-performance liquid chromatography. The phylogenetic tree and PLS-DA were combined to correlate genetic and chemical differences among L. japonicus derived from different origins. The results showed that the concatenation sequence could distinguish among L. japonicus from different origins. Moreover, chemical analysis revealed intergroup differences, but the results were not of sufficiently high quality as that of molecular phylogeny. Furthermore, the results of combined chemical and phylogenetic analyses suggested that differences in metabolites are influenced by not only genetic differences but also environmental factors. These results provide valuable information for the artificial cultivation of L. japonicus and new ideas for improving its quality.

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Section: Introductionmentioning

confidence: 99%

Relevance of genetic and active ingredient content differences in Leonurus japonicus Houtt from different origins

Han

Wen

Chen

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

Relevance of genetic and active ingredient content differences in Leonurus japonicus Houtt from different origins

Han

Wen

Chen

et al. 2023

Genet Resour Crop Evol

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On the identity of the species Pulsatilla astragalifolia (Ranunculaceae)

Zaikov,

Shmakov

2023

bpssm

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Pulsatilla is a genus of early-flowering, ornamental, partly medicinal plants, characterized by a high level of hybridization. In this connection, many of them are rare and endemic to individual territories. Several different nomenclature combinations have been made over the extensive history of the genus revising, which often causes debates between scientists. One of the cases is the taxon Pulsatilla astragalifolia Pobed. To try to solve this issue, ITS fragments of nuclear DNA of three close species (Pulsatilla bungeana C. A. Mey. ex Ledeb., P astragalifolia Pobed., P tenuiloba (Hayek) Juz.) were sequenced. Another group is formed by several clades with samples of P. bungeana and P. astragalifolia, According to the results obtained, the studied samples were divided into several groups. One group consists of two clades: the first - P. tenuiloba samples, the second - P. astragalifolia samples. Which may indicate the presence of several forms of these species. Subsequently, it is planned to study new occurrences of these species, as well as the analysis of the other DNA fragments. However, the findings indicate that Pulsatilla astragalifolia is a separate species, having both morphological and genetic differences from related species.

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Phylogenetic relationships and status of taxa of Pulsatilla uralensis and P. patens s.str. (Ranunculaceae) in north-eastern European Russia

Cited by 3 publications

References 17 publications

Relevance of genetic and active ingredient content differences in Leonurus japonicus Houtt from different origins

Relevance of genetic and active ingredient content differences in Leonurus japonicus Houtt from different origins

Relevance of genetic and active ingredient content differences in Leonurus japonicus Houtt from different origins

On the identity of the species Pulsatilla astragalifolia (Ranunculaceae)

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