Evolutionarily conserved plant genes responsive to root-knot nematodes identified by comparative genomics

Mota, Ana Paula Zotta; Fernandez, Diana; Arraes, Fabrício Barbosa Monteiro; Petitot, Anne‐Sophie; Melo, Bruno Paes de; de, Maria Eugênia Lisei; Grynberg, Priscila; Saraiva, Mario Alfredo Passos; Guimarães, P. M.; Brasileiro, Ana Cristina Miranda; Albuquerque, Érika Valéria Saliba; Danchin, Étienne; Grossi-de-Sá, Maria Fátima

doi:10.1007/s00438-020-01677-7

Cited by 14 publications

(23 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These 44 Arabidopsis thaliana genes were considered reliable markers of the priming phase through meta-analysis of RNA-Seq data obtained during different priming conditions and in different plant species. For the orthologs search, we explored a set of protein orthogroups shared among 22 plant species, including A. duranensis , previously characterized by our team [ 54 ]. This comparative analysis allowed the identification of orthologs between the A. thaliana universal gene markers [ 8 ] and the A. duranensis gene models significantly differentially expressed (FDR < 0.05) in response to UV ( Table S1 ).…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, most of the 200 genes belonging to the above priming-markers orthogroups displayed the same trend (up- or downregulation) as the other four plant species (banana, tomato, grapevine, and A. thaliana ) primed with four different stimuli, according to [ 8 ]. Interestingly, two of the three largest orthogroups (OG0000001 and OG0000057) encoding both for protein kinases ( Table S4 ) were also previously identified as orthogroups commonly regulated upon nematode infection in four RKN-resistant plant genotypes ( A. stenosperma, coffee, soybean, and rice) [ 54 ]. Furthermore, we identified ten metaDEGs belonging to six orthogroups shared with A. thaliana universal gene markers ( Figure 6 ; Table 1 ), suggesting that they are regulated at the priming phase in A. stenosperma.…”

Section: Resultsmentioning

confidence: 99%

“…To find A. duranensis orthologs of the 44 universal A. thaliana gene markers of plant priming [ 8 ], we examined a set of 35,238 protein orthogroups, previously characterized by our team [ 54 ]. These groups of putatively orthologous genes (orthogroups) were inferred by the comparative analysis across the predicted proteomes of 22 plant species, including A. thaliana and A. duranensis , using the OrthoFinder v1 software [ 111 ].…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Transcriptome Responses of Wild Arachis to UV-C Exposure Reveal Genes Involved in General Plant Defense and Priming

Martins

Mota

Carvalho

et al. 2022

Plants

Self Cite

View full text Add to dashboard Cite

Stress priming is an important strategy for enhancing plant defense capacity to deal with environmental challenges and involves reprogrammed transcriptional responses. Although ultraviolet (UV) light exposure is a widely adopted approach to elicit stress memory and tolerance in plants, the molecular mechanisms underlying UV-mediated plant priming tolerance are not fully understood. Here, we investigated the changes in the global transcriptome profile of wild Arachis stenosperma leaves in response to UV-C exposure. A total of 5751 differentially expressed genes (DEGs) were identified, with the majority associated with cell signaling, protein dynamics, hormonal and transcriptional regulation, and secondary metabolic pathways. The expression profiles of DEGs known as indicators of priming state, such as transcription factors, transcriptional regulators and protein kinases, were further characterized. A meta-analysis, followed by qRT-PCR validation, identified 18 metaDEGs as being commonly regulated in response to UV and other primary stresses. These genes are involved in secondary metabolism, basal immunity, cell wall structure and integrity, and may constitute important players in the general defense processes and establishment of a priming state in A. stenosperma. Our findings contribute to a better understanding of transcriptional dynamics involved in wild Arachis adaptation to stressful conditions of their natural habitats.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Transcriptome Responses of Wild Arachis to UV-C Exposure Reveal Genes Involved in General Plant Defense and Priming

Martins

Mota

Carvalho

et al. 2022

Plants

Self Cite

View full text Add to dashboard Cite

show abstract

“…Many studies of nematode resistance have indicated the action of few genes with greater effect (Passianotto, et al, 2017;Mota, et al, 2020). There are also other results, that indicate the action of lesser effect genes that have evolved over time, according to the gene-for-gene theory (Bell et al, 2019;Przybylska;Obrępalska-Stęplowska, 2020).…”

Section: Resultsmentioning

confidence: 99%

Resistance of new Coffea canephora clones to root-knot nematode (Meloidogyne incognita) in the western amazon

Rudnick

Junior

Fernandes

et al. 2020

View full text Add to dashboard Cite

Root-knot disease is among the main diseases affecting coffee crop. The plant selection to the development new resistant cultivars is among one the most efficient methods of control. The present work aimed to quantify the resistance responses of Coffea canephora clones to root-knot nematode Meloidogyne incognita in the Western Amazon. For this, 17 previously selected clones were evaluated in three experimental trials, carried out in the municipalities of Ji-Paraná and Porto Velho, Rondônia. The resistance to root-knot nematodes M. incognita were evaluated by the numbers of gall in the roots (NG) and by the reproductive factor (RF). The resistance response was also interpreted according the genetic diversity of the clones based in their morphological traits. The clones BRS3210, C12, BRS2314, BRS3137 and BRS1216 are resistant to M. incognita with RF of 0.34, 0.62, 0.79, 0.86 and 0.98, respectively. BRS3213, C125, C15, BRS2336, BRS3220 and C09 clones were classified as susceptible, with RF of 1.93, 1.95, 2.00, 2.31, 2.32 and 2.35. The BRS3193, C160 and BRS2357 clones were classified as very susceptible, with RF values of 3.03, 4.41 and 5.82, respectively. The clustering based on the genetic diversity of morphological traits indicated that genotypes more similar to the Robusta botanic variety had lower RF. The hybrid plants showed intermediate degrees of resistance indicating the segregation for the character of the M. incognita resistance. The clones BRS3210, C12, BRS2299, BRS2314, BRS3137 and BRS1216 expressed resistance responses to M. incognita with potential for growing resistant genotypes in the Western Amazon.

show abstract

“…In line with these data, research will have to fully consider comparative genomics within the Ma locus to identify the resistance determinants (sequences and/or motifs) and in particular, those involved in the resistance specificity. Methods and model studies published recently open the way in this objective [75][76][77]. The noteworthy C-terminal part of the gene with a huge post-LRR region (=5 repeated PL domains) makes it the longest R gene ever cloned.…”

Section: Discussionmentioning

confidence: 99%

Deciphering Resistance to Root-Knot Nematodes in Prunus for Rootstock Breeding: Sources, Genetics and Characterization of the Ma Locus

Esmenjaud¹

2021

Horticulturae

View full text Add to dashboard Cite

Root-knot nematode (RKN) species are predominant pests of crops, attacking stone fruit crops Prunus spp. under Mediterranean climate conditions worldwide. Natural resistance for rootstock breeding is a control method that is gaining interest as an alternative to the highly toxic nematicides. This review first reports an outline of the root-knot nematodes parasitizing stone fruit crops and the Prunus species and rootstocks. It then describes the main sources of resistance detected among the Prunus germplasm and focuses on the major resistance genes identified and their characteristics (spectrum, durability, histological mechanism, effect of temperature, interaction with other pests and diseases, etc.). In peach, besides the RMia reference gene, the new genes PkMi and Mf, also located on chromosome 2, need to be characterized regarding their spectrum and relationship. The two other Prunus reference genes, Ma from plum (complete spectrum) and RMja from almond (more restricted spectrum), are orthologs that belong to a TIR-NB-LRR (TNL) cluster on chromosome 7. The review finally summarizes the positional cloning of the Ma gene and the characterization of its unique TNL structure, encompassing a five-times repeated post-LRR domain. Deciphering how this structure is functionally involved in Ma’s remarkable biological properties is a real challenge for the future.

show abstract

Evolutionarily conserved plant genes responsive to root-knot nematodes identified by comparative genomics

Cited by 14 publications

References 63 publications

Transcriptome Responses of Wild Arachis to UV-C Exposure Reveal Genes Involved in General Plant Defense and Priming

Transcriptome Responses of Wild Arachis to UV-C Exposure Reveal Genes Involved in General Plant Defense and Priming

Resistance of new Coffea canephora clones to root-knot nematode (Meloidogyne incognita) in the western amazon

Deciphering Resistance to Root-Knot Nematodes in Prunus for Rootstock Breeding: Sources, Genetics and Characterization of the Ma Locus

Contact Info

Product

Resources

About