Evolution of the serine β-lactamases: past, present and future

Hall, Barry G.; Barlow, Miriam

doi:10.1016/j.drup.2004.02.003

Cited by 259 publications

(170 citation statements)

References 31 publications

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“…enabled detailed predictions as to the future evolution of antibiotic resistance (Barlow and Hall 2003;Hall and Barlow 2004;Weinreich et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Adaptive Divergence in Experimental Populations of Pseudomonas fluorescens. III. Mutational Origins of Wrinkly Spreader Diversity

Bantinaki

Kassen²,

Knight³

et al. 2007

Genetics

155

238

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Understanding the connections among genotype, phenotype, and fitness through evolutionary time is a central goal of evolutionary genetics. Wrinkly spreader (WS) genotypes evolve repeatedly in model Pseudomonas populations and show substantial morphological and fitness differences. Previous work identified genes contributing to the evolutionary success of WS, in particular the di-guanylate cyclase response regulator, WspR. Here we scrutinize the Wsp signal transduction pathway of which WspR is the primary output component. The pathway has the hallmarks of a chemosensory pathway and genetic analyses show that regulation and function of Wsp is analogous to the Che chemotaxis pathway from Escherichia coli. Of significance is the methyltransferase (WspC) and methylesterase (WspF) whose opposing activities form an integral feedback loop that controls the activity of the kinase (WspE). Deductions based on the regulatory model suggested that mutations within wspF were a likely cause of WS. Analyses of independent WS genotypes revealed numerous simple mutations in this single open reading frame. Remarkably, different mutations have different phenotypic and fitness effects. We suggest that the negative feedback loop inherent in Wsp regulation allows the pathway to be tuned by mutation in a rheostat-like manner.

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“…enabled detailed predictions as to the future evolution of antibiotic resistance (Barlow and Hall 2003;Hall and Barlow 2004;Weinreich et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Adaptive Divergence in Experimental Populations of Pseudomonas fluorescens. III. Mutational Origins of Wrinkly Spreader Diversity

Bantinaki

Kassen²,

Knight³

et al. 2007

Genetics

155

238

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“…ATB resistance genes exist naturally in the environment owing to a range of selective pressures in nature but the environmental reservoirs of resistance determinants are poorly understood (Allen et al 2010). Evidence shows that ATB resistance genes are common in natural environments and existed, even on plasmids, before the use of ATBs (Hall & Barlow 2004). Furthermore, it is generally known that the heavy-metal resistance and resistance against ATBs are often carried on the same bacterial plasmids or transposons (Vranes et al 1994); therefore the selected actinobacterial isolates were also tested for their resistance against some kinds of ATBs.…”

Section: Metabolic Activity Of Selected Actinobacteriamentioning

confidence: 99%

Actinobacteria occurrence and their metabolic characteristics in the nickel-contaminated soil sample

et al. 2014

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Heavy metals are a significant source of pollution in soils that have been demonstrated to exert significant toxic effect on soil microbial assemblages. Here we investigate the occurrence and metabolic characteristics of actinobacteria, which form a predominated component of farmland bacterial community near the town of Sereď in southwest Slovakia, contaminated by close nickel ore facility. Actinobacteria occurred in this environment with high concentrations of nickel (2.109 mg/kg), slightly above the natural occurrence of cobalt (355 mg/kg) and zinc (177 mg/kg), even too low concentration of iron (35.75 mg/kg) for a normal soil and not a toxic amount of copper (32.2 mg/kg) and cadmium (<0.25 mg/kg). The phylogeny was reconstructed using partial sequences of 16S rDNA genes of both, actinobacterial isolates and clones. A total of 105 actinobacterial representatives were divided into 66 species belonging to one order, 7 genera and 5 uncategorized groups. The selected 14 morphologically distinct isolates were able to produce drop collapsing, haemolytic and lipase activities. Whereas only 4 isolates produced dark brown melanin pigment and only 5 of them produced decolourization of the azo dye, all isolates tested were capable of assimilating all 11 sugars tested. All these actinobacterial isolates were resistant to nickel, cobalt, zinc, cadmium, copper, but the level of resistance differed between the individual isolates, and the resistance profiles of antibiotics (gentamycin, ampicillin, ciprofloxacin, chloramphenicol, erythromycin, rifampicin, penicillin-G) varied among them to a certain extent. Our results suggested that actinobacteria in soil contaminated by nickel present a relatively divergent group inside of microbial assemblage.

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“…The extraordinary ability of microbes to acquire antibiotic resistance is easier to understand when analyzed from an evolutionary perspective. Thus, while the use of antibiotics as therapeutics started less than 70 years ago, bacterial resistance mechanisms have been coevolving with natural antimicrobial compounds for billions of years, as recent studies of the evolution of ␤-lactamases clearly demonstrated (69,81). Despite this, the spread of resistance was limited because resistant strains are often less virulent and, consequently, less competitive than the sensitive strains from which they originated in the absence of selective pressure.…”

mentioning

confidence: 99%

Adaptive and Mutational Resistance: Role of Porins and Efflux Pumps in Drug Resistance

2012

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SUMMARY The substantial use of antibiotics in the clinic, combined with a dearth of new antibiotic classes, has led to a gradual increase in the resistance of bacterial pathogens to these compounds. Among the various mechanisms by which bacteria endure the action of antibiotics, those affecting influx and efflux are of particular importance, as they limit the interaction of the drug with its intracellular targets and, consequently, its deleterious effects on the cell. This review evaluates the impact of porins and efflux pumps on two major types of resistance, namely, mutational and adaptive types of resistance, both of which are regarded as key phenomena in the global rise of antibiotic resistance among pathogenic microorganisms. In particular, we explain how adaptive and mutational events can dramatically influence the outcome of antibiotic therapy by altering the mechanisms of influx and efflux of antibiotics. The identification of porins and pumps as major resistance markers has opened new possibilities for the development of novel therapeutic strategies directed specifically against these mechanisms.

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Evolution of the serine β-lactamases: past, present and future

Cited by 259 publications

References 31 publications

Adaptive Divergence in Experimental Populations of Pseudomonas fluorescens. III. Mutational Origins of Wrinkly Spreader Diversity

Adaptive Divergence in Experimental Populations of Pseudomonas fluorescens. III. Mutational Origins of Wrinkly Spreader Diversity

Actinobacteria occurrence and their metabolic characteristics in the nickel-contaminated soil sample

Adaptive and Mutational Resistance: Role of Porins and Efflux Pumps in Drug Resistance

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