Metals made by additive manufacturing (AM) have intensely augmented over the past decade for customizing complex structured products in the aerospace industry, automotive, and biomedical engineering. However, for AM fabricated steels, the correlation between the microstructure and mechanical properties is yet a challenging task with limited reports. To realize optimization and material design during the AM process, it is imperative to understand the influence of the microstructural features on the mechanical properties of AM fabricated steels. In the present study, three material blocks with 120×25×15 mm3 dimensions are produced from PH1 steel powder using powder bed fusion (PBF) technology to investigate the anisotropic plastic deformation behavior arising from the manufacturing process. Despite being identical in geometrical shape, the manufactured blocks are designed distinguishingly with various coordinate transformations, i.e. alternating the orientation of the block in the building direction (z) and the substrate plate (x, y). Uniaxial tensile tests are performed along the length direction of each specimen to characterize the anisotropic plastic deformation behavior. The distinctly anisotropic plasticity behavior in terms of strength and ductility are observed in the AM PH1 steel, which is explained by their varied microstructure affected by the thermal history of blocks. It could also be revealed that the thermal history in the AM blocks is influenced by the block geometry even though the same process parameters are employed.