“…Among these, double-digest RADseq, or ddRADseq (Peterson et al, 2012), is highly customizable as regards the final number of loci, depending on the choice of enzymes and range of fragment size selected. The ddRADseq approach has been applied with success to many purposes including population genetic studies (Kjeldsen et al, 2016; Black, Seears, Hollenbeck, & Samollow, 2017; Sherpa, Rioux, Goindin, et al, 2018), phylogenetic reconstructions (DaCosta & Sorenson, 2016; Vargas, Ortiz, & Simpson, 2017; Boubli et al, 2018; Lee et al, 2018; Sherpa, Rioux, Pougnet-Lagarde, & DesprĂ©s, 2018), demographic inferences (Capblancq, DesprĂ©s, Rioux, & MavĂĄrez, 2015; Nunziata, Lance, Scott, Lemmon, & Weisrock, 2017; Settepani et al, 2017; Elleouet & Aitken, 2018) and landscape genetic analyses (Saenz-Agudelo et al, 2015; Johnson, Gaddis, Cairns, Konganti, & Krutovsky, 2017). Despite the recognized advantages of the ddRADseq technique, several limitations and weaknesses arose in the literature (Davey et al, 2013; K. R. Andrews et al, 2016; Lowry et al, 2017).…”