Evolution of infection in mice inoculated by the oral route with different developmental forms of Trypanosoma cruzi I and II

Dias, Greicy Brisa Malaquias; Gruendling, Ana Paula; Araújo, Silvana Márques de; Gomes, Mônica Lúcia; Toledo, Max Jean de Ornelas

doi:10.1016/j.exppara.2013.08.013

Cited by 36 publications

(27 citation statements)

References 25 publications

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“…17,18 The parasitological parameters showed that Ca, Co, and Ly altered the course of infection compared with CI. A previous study also found that the prepatent period was significantly longer in animals that were infected with the Y strain 25 of T. cruzi and treated with Ly compared with an infected and untreated control group. This increase in the prepatent period is beneficial to infected animals.…”

Section: Discussionmentioning

confidence: 73%

Highly diluted medication reduces tissue parasitism and inflammation in mice infected by Trypanosoma cruzi

et al. 2016

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Section: Discussionmentioning

confidence: 73%

Highly diluted medication reduces tissue parasitism and inflammation in mice infected by Trypanosoma cruzi

et al. 2016

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“…Mortality rate in orally infected patients is reported as higher (8–35%) when compared to the classical vectorial transmission, through triatomine excreta deposition after biting (<5–10%) [14]. It is well known that both trypomastigotes and metacyclic trypomastigotes are associated with oral Chagas transmission [15–17]. Regarding T .…”

Section: Introductionmentioning

confidence: 99%

Unraveling Chagas disease transmission through the oral route: Gateways to Trypanosoma cruzi infection and target tissues

et al. 2017

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Oral transmission of Trypanosoma cruzi, the causative agent of Chagas disease, is the most important route of infection in Brazilian Amazon and Venezuela. Other South American countries have also reported outbreaks associated with food consumption. A recent study showed the importance of parasite contact with oral cavity to induce a highly severe acute disease in mice. However, it remains uncertain the primary site of parasite entry and multiplication due to an oral infection. Here, we evaluated the presence of T. cruzi Dm28c luciferase (Dm28c-luc) parasites in orally infected mice, by bioluminescence and quantitative real-time PCR. In vivo bioluminescent images indicated the nasomaxillary region as the site of parasite invasion in the host, becoming consistently infected throughout the acute phase. At later moments, 7 and 21 days post-infection (dpi), luminescent signal is denser in the thorax, abdomen and genital region, because of parasite dissemination in different tissues. Ex vivo analysis demonstrated that the nasomaxillary region, heart, mandibular lymph nodes, liver, spleen, brain, epididymal fat associated to male sex organs, salivary glands, cheek muscle, mesenteric fat and lymph nodes, stomach, esophagus, small and large intestine are target tissues at latter moments of infection. In the same line, amastigote nests of Dm28c GFP T. cruzi were detected in the nasal cavity of 6 dpi mice. Parasite quantification by real-time qPCR at 7 and 21 dpi showed predominant T. cruzi detection and expansion in mouse nasal cavity. Moreover, T. cruzi DNA was also observed in the mandibular lymph nodes, pituitary gland, heart, liver, small intestine and spleen at 7 dpi, and further, disseminated to other tissues, such as the brain, stomach, esophagus and large intestine at 21 dpi. Our results clearly demonstrated that oral cavity and adjacent compartments is the main target region in oral T. cruzi infection leading to parasite multiplication at the nasal cavity.

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“…In a dog experimental model, animals presented enhanced cardiac parasitism and inflammatory response when infected with bTp, suggesting that the inoculum source affects the immunopathological aspects of Chagas disease (de Souza et al, 2014). In addition, it was also demonstrated in a mouse model of infection that bTps were more virulent than culture mTps, via both oral and peritoneal inoculation (Dias et al, 2013), concluding and remarking on the importance of the stage used, its origin, and the model of the infection in order to study the immune response.…”

Section: Discussionmentioning

confidence: 98%

“…In a comparative study between mTp and bTp, Dias et al (2013) have demonstrated that the infection by intraperitoneal or oral inoculation of bTp is more virulent than with mTp. Specifically, animals infected with bTp presented higher parasitemia and mortality in comparison to infection with mTp (Dias et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Modulatory Effect of Trypanosoma cruzi Infective Stages in Different Dendritic Cell Populations in vitro

Gutierrez

Lammel

Ramirez

et al. 2020

Front. Cell. Infect. Microbiol.

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Trypanosoma cruzi is a protozoan parasite that infects at least 7 million persons in the world (OMS, 2019). In endemic areas, infection normally occurs by vectorial transmission; however, outside, it normally happens by blood and includes congenital transmission. The persistence of T. cruzi during infection suggests the presence of immune evasion mechanisms and the modulation of the anti-parasite response to a profile incapable of eradicating the parasite. Dendritic cells (DCs) are a heterogeneous population of antigen-presenting cells (APCs) that patrol tissues with a key role in mediating the interface between the innate and adaptive immune response. Previous results from our lab and other groups have demonstrated that T. cruzi modulates the functional properties of DCs, in vitro and in vivo. During vectorial transmission, metacyclic (m) trypomastigotes (Tps) eliminated along with the insect feces reach the mucous membranes or injured skin. When transmission occurs by the hematic route, the parasite stage involved in the infection is the circulating or blood (b) Tp. Here, we studied in vitro the effect of both infective mTp and bTp in two different populations of DCs, bone marrow-derived DCs (BMDCs) and XS106, a cell line derived from epidermal DCs. Results demonstrated that the interaction of both Tps imparts a different effect in the functionality of these two populations of DCs, suggesting that the stage of T. cruzi and DC maturation status could define the immune response from the beginning of the ingress of the parasite, conditioning the course of the infection.

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Evolution of infection in mice inoculated by the oral route with different developmental forms of Trypanosoma cruzi I and II

Cited by 36 publications

References 25 publications

Highly diluted medication reduces tissue parasitism and inflammation in mice infected by Trypanosoma cruzi

Highly diluted medication reduces tissue parasitism and inflammation in mice infected by Trypanosoma cruzi

Unraveling Chagas disease transmission through the oral route: Gateways to Trypanosoma cruzi infection and target tissues

Modulatory Effect of Trypanosoma cruzi Infective Stages in Different Dendritic Cell Populations in vitro

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