Evolution of a Double Amino Acid Substitution in the 5-Enolpyruvylshikimate-3-Phosphate Synthase in <i>Eleusine indica</i> Conferring High-Level Glyphosate Resistance

Yu, Qin; Jalaludin, Adam; Han, Heping; Chen, Ming; Sammons, R. Douglas; Powles, Stephen B.

doi:10.1104/pp.15.00146

Cited by 201 publications

(359 citation statements)

References 44 publications

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“…According to Carvalho et al (2012), sourgrass may present mechanisms that contribute to glyphosate resistance, including reduced glyphosate absorption and translocation, metabolism into nontoxic substances, or target-site mutations in the EPSPS gene. Others grass weed species, such as Echinochloa colona (Alarcón-Reverte et al, 2015), E. indica (Yu et al, 2015) and Lolium perenne ssp. multiflorum (Salas, Scott, Dayan, & Burgos, 2015), exhibited an RF ranging from 1.4 to 31.1 based on GR 50 and from 4 to 15 based on LD 50 , except for E. indica, which showed an FR that was > 182 (Yu et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

<b>Glyphosate efficacy on sourgrass biotypes with suspected resistance collected in GR-crop fields

et al. 2018

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ABSTRACT. In Brazil, infestations of crop areas with glyphosate-resistant (GR) sourgrass (Digitaria insularis (L.) Fedde) biotypes has risen significantly, increasing crop production costs. Glyphosate efficacy on three biotypes (GO, BA and MT) of sourgrass with suspected resistance was evaluated. A susceptible biotype (MG) was used as the control. The results confirmed that the MG and GO biotypes were susceptible to glyphosate (control > 90%). The MG biotype exhibited growth reduction and mortality by 50% (GR 50 and LD 50 , respectively) with mean glyphosate doses of 243.7 and 431.6 g ae ha -1. The resistance index of the biotypes with suspected resistance ranged from 2.8 to 6.1 in relation to GR 50 and between 1.4 to 26.7 in relation to LD 50 . The glyphosate susceptibility ranking of the sourgrass biotypes was MG < GO < MT < BA. The MT and BA biotypes demonstrated high glyphosate resistance levels, and the GO biotype had a high potential to develop resistance. Farmers should avoid the application of glyphosate overdoses to minimize the selection pressure on weeds. Eficácia do glyphosate em biótipos de capim-amargoso com indício de resistência coletadas em cultivos RR RESUMO. No Brasil, as infestações de áreas agrícolas com biótipos de capim-amargoso resistentes ao glyphosate, resultam em aumento significativo nos custos de produção das culturas. Avaliou-se a eficácia do glyphosate em três biótipos de capim-amargoso com indício de resistência (GO, BA e MT) a este herbicida. Um biótipo suscetível (MG) foi utilizado como controle. Constatou-se que os biótipos MG e GO foram suscetíveis ao herbicida (controle > 90%). O biótipo MG apresentou redução do crescimento e mortalidade em 50% (GR 50 e C 50 , respectivamente) nas doses de glyphosate de 243,7 e 431,6 g ha -1. Os fatores de resistência dos biótipos com indício de resistência oscilaram entre 2,8 a 6,1 em relação ao GR 50 ; e entre 1,4 e ≥ 26,7 em relação ao C 50 . A ordem de susceptibilidade dos biótipos de capim-amargoso foi MG < GO < MT < BA. Estes últimos apresentaram elevado nível de resistência ao glyphosate, e o biótipo GO têm potencial para desenvolvê-la. Portanto, os agricultores devem evitar a aplicação de superdose de glyphosate a fim de reduzir a pressão de seleção das plantas daninhas.Palavras-chave: controle químico, Digitaria insularis, dose-resposta, fator de resistência.

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Section: Discussionmentioning

confidence: 99%

<b>Glyphosate efficacy on sourgrass biotypes with suspected resistance collected in GR-crop fields

et al. 2018

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“…Such single mutation confers intermediate levels of glyphosate resistance (RF = 2 to 4). However, double mutation at positions 106 (T102I and P106S) of the mutant population can confer higher levels of resistance (RF>10) (Yu et al, 2015). Nevertheless, the mechanism that confers resistance in populations from Brazil is still unknown.…”

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confidence: 99%

Goosegrass Resistant to Glyphosate in Brazil

et al. 2017

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-Several cases of herbicide resistance in goosegrass have been confirmed worldwide. Reports of control failures after glyphosate application have been observed, especially in the Midwest region of Paraná State. The objective of this study was to evaluate the existence of goosegrass populations resistant to glyphosate. For this, 25 populations collected in two consecutive seasons (2013/ 2014 and 2014/2015) were sown and grown in greenhouse. Glyphosate dose-response curve experiments were performed using doses of 0, 60, 120, 240, 480, 960, 1,920, 3,840, 7,680 and 15,360 g a.e. ha -1 . The application stages were from two to three tillers (E1) for the populations of 2013/2014 and E1 and five to six tillers (E2) for the populations of 2014/2015. Furthermore, three of the populations supposedly considered resistant in these experiments (populations 7, 19 and 25) have had their F1 submitted to the herbicide dose-response test in order to verify whether the resistance was inheritable. With the results obtained in this study, it was concluded that the populations 19 and 25, from Campo Mourão and Luziânia (Midwest of Paraná) are the first confirmed cases of goosegrass resistant to glyphosate in Brazil (RF = 3.99 to 6.81), following all the criteria for confirmation of new weed resistance cases. (2013/2014 e 2014/2015) foram semeadas e cultivadas em casa de vegetação. Experimentos de curva de dose-resposta de glyphosate foram realizados utilizando-se as doses de 0, 60, 120, 240, 480, 960, 1.920, 3.840, 7.680 e 15.360 g e.a. ha -1 Keywords: Eleusine indica, dose-response, application timming, resistance factor. RESUMO -Diversos casos de resistência de capim-pé-de-galinha têm

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“…In crop fields under glyphosate selection, the evolution of a double amino acid substitution at Thr-102 and Pro-106 in the EPSPS in Eleusine indica conferring a high level of glyphosate resistance was recently communicated (Yu et al, 2015). In this species, other mechanisms of glyphosate resistance have also been found, associated with the over-expression of EPSPS .…”

Section: Introductionmentioning

confidence: 96%

Mechanism of Resistance to Glyphosate in Lolium perenne from Argentina

et al. 2017

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In Argentina, glyphosate resistance was reported in a Lolium perenne population after 12 years of successful herbicide use. The aim of the current paper was to put in evidence for the mechanism of glyphosate resistance of this weed. Susceptible leaves treated with different doses of glyphosate and incubated in vitro showed an accumulation of shikimic acid of around three to five times the basal level, while no changes were detected in leaves of glyphosate-resistant plants. The resistance mechanism prevents shikimate accumulation in leaves, even under such tissue-isolation conditions. The activity of the glyphosate target enzyme (EPSPS: 5-enolpyruvylshikimate-3-phosphate synthase) was quantified at different herbicide concentrations. EPSPS from resistant plants showed no difference in glyphosate-sensitivity compared to EPSPS from susceptible plants, and, accordingly, no amino acid substitution causing mutations associated with resistance were found. While the glyphosate target enzymes were equally sensitive, the basal EPSPS activity in glyphosate resistant plants was approximately 3-fold higher than the EPSPS activity in susceptible plants. This increased EPSPS activity in glyphosate resistant plants was associated with a 15-fold higher expression of EPSPS compared with susceptible plants. Therefore, the over-expression of EPSPS appears to be the main mechanism responsible for resistance to glyphosate. This mechanism has a constitutive character and has important effects on plant fitness, as recently reported.

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Evolution of a Double Amino Acid Substitution in the 5-Enolpyruvylshikimate-3-Phosphate Synthase in Eleusine indica Conferring High-Level Glyphosate Resistance

Cited by 201 publications

References 44 publications

<b>Glyphosate efficacy on sourgrass biotypes with suspected resistance collected in GR-crop fields

<b>Glyphosate efficacy on sourgrass biotypes with suspected resistance collected in GR-crop fields

Goosegrass Resistant to Glyphosate in Brazil

Mechanism of Resistance to Glyphosate in Lolium perenne from Argentina

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