Evolution and Diversity of the Golgi

Klute, Mary J.; Melançon, Paul; Dacks, Joel B.

doi:10.1101/cshperspect.a007849

Cited by 58 publications

(44 citation statements)

References 80 publications

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“…This basic secretory function is mediated by stacks of flattened cisternal membranes. Although the arrangement of cisternae into stacks is a common feature found in most eukaryotes (1), the morphological organization of stacks differs between species. Most organisms have numerous copies of stacks, present as individual units that are scattered throughout the cytoplasm (2).…”

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confidence: 99%

Mitotic Golgi disassembly is required for bipolar spindle formation and mitotic progression

Guizzunti

Seemann

2016

Proc. Natl. Acad. Sci. U.S.A.

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During mitosis, the mammalian Golgi vesiculates and, upon partitioning, reassembles in each daughter cell; however, it is not clear whether the disassembly process per se is important for partitioning or is merely an outcome of mitotic entry. Here, we show that Golgi vesiculation is required for progression to metaphase. To prevent Golgi disassembly, we expressed HRP linked to a Golgi-resident protein and acutely triggered the polymerization of 3,3′-diaminobenzidine (DAB) in the Golgi lumen. The DAB polymer does not affect interphase cell viability, but inhibits Golgi fragmentation by nocodazole and brefeldin A and also halts cells in early mitosis. The arrest is Golgi specific and does not occur when DAB is polymerized in the endosomes. Cells with a DAB polymer in the Golgi enter mitosis normally but arrest with an intact Golgi clustered at a monopolar spindle and an active spindle assembly checkpoint (SAC). Mitotic progression is restored upon centrosome depletion by the Polo-like kinase 4 inhibitor, centrinone, indicating that the link between the Golgi and the centrosomes must be dissolved to reach metaphase. These results demonstrate that Golgi disassembly is required for mitotic progression because failure to vesiculate the Golgi activates the canonical SAC. This requirement suggests that cells actively monitor Golgi integrity in mitosis.

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confidence: 99%

Mitotic Golgi disassembly is required for bipolar spindle formation and mitotic progression

Guizzunti

Seemann

2016

Proc. Natl. Acad. Sci. U.S.A.

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“…olgi traffic is an ancient process (Klute et al 2011). Certain core mechanisms are likely to be conserved in most or all eukaryotes, but these core mechanisms have undoubtedly been enhanced or modified during evolution.…”

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Models for Golgi Traffic: A Critical Assessment

Glick¹,

Luini²

2011

Cold Spring Harbor Perspectives in Biology

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181

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“…If this is true, then conservation of Golgi morphology among eukaryotes may also be a consequence of the above robustness. Total loss of the Golgi apparatus in several organisms can be regarded as nonresponses (12). Considering the reverse process of Golgi breakdown, formation of Golgi morphology from the endomembrane system can also be an abrupt transition.…”

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confidence: 99%

Golgi apparatus self-organizes into the characteristic shape via postmitotic reassembly dynamics

Tachikawa

Mochizuki

2017

Proc. Natl. Acad. Sci. U.S.A.

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The Golgi apparatus is a membrane-bounded organelle with the characteristic shape of a series of stacked flat cisternae. During mitosis in mammalian cells, the Golgi apparatus is once fragmented into small vesicles and then reassembled to form the characteristic shape again in each daughter cell. The mechanism and details of the reassembly process remain elusive. Here, by the physical simulation of a coarse-grained membrane model, we reconstructed the threedimensional morphological dynamics of the Golgi reassembly process. Considering the stability of the interphase Golgi shape, we introduce two hypothetical mechanisms-the Golgi rim stabilizer protein and curvature-dependent restriction on membrane fusioninto the general biomembrane model. We show that the characteristic Golgi shape is spontaneously organized from the assembly of vesicles by proper tuning of the two additional mechanisms, i.e., the Golgi reassembly process is modeled as self-organization. We also demonstrate that the fine Golgi shape forms via a balance of three reaction speeds: vesicle aggregation, membrane fusion, and shape relaxation. Moreover, the membrane fusion activity decreases thickness and the number of stacked cisternae of the emerging shapes.Golgi apparatus | self-organization | physical biology modeling | computer simulation

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Evolution and Diversity of the Golgi

Cited by 58 publications

References 80 publications

Mitotic Golgi disassembly is required for bipolar spindle formation and mitotic progression

Mitotic Golgi disassembly is required for bipolar spindle formation and mitotic progression

Models for Golgi Traffic: A Critical Assessment

Golgi apparatus self-organizes into the characteristic shape via postmitotic reassembly dynamics

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