Evolução da técnica de PCR: sua contribuição no diagnóstico da infecção por HPV

Menêses, Marta Soraia L.; Toralles, Maria Betânia Pereira; Mendes, Carlos Maurício Cardeal

doi:10.9771/cmbio.v18i3.34480

Cited by 4 publications

(1 citation statement)

References 20 publications

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“…In the diagnosis of HPV, the in situ hybridization methodology helps with the histopathological results and is also a technique that uses tissue samples in a wide range. sensitivity and specificity, even with little material (MENÊSES et al, 2019).…”

Section: Means Of Detectionmentioning

confidence: 99%

Health in focus: HPV

Carvalho¹,

Casimiro²,

Demberg³

et al. 2023

Development and Its Applications in Scientific Knowledge

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The present study is concerned with discussing the infection caused by the human papillomavirus (HPV), going through themes about the means of transmission, detection, most common signs and symptoms, strategies for promotion and prevention, and treatment in Primary Care. In addition, it is concerned with exposing the interventions of nursing professionals in the Primary Care scenario. The bibliographic survey was carried out through scientific articles found in the electronic databases Scielo and Google Scholar. Additionally, magazines with the theme of interest. We opted, preferably, for works prepared in the period from 2018 to 2023. The lack of information has been favoring excessive propagation and putting the health of the population at risk. Thus, in observing a deficiency in the knowledge of this virus, it is expected to amplify knowledge, considering that it represents a considerable public health problem.

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Section: Means Of Detectionmentioning

confidence: 99%

Health in focus: HPV

Carvalho¹,

Casimiro²,

Demberg³

et al. 2023

Development and Its Applications in Scientific Knowledge

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Molecular methods for diagnosis of monkeypox: A mini-review

Thomasi¹,

Correa²,

Carmo³

et al. 2022

Preprint

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Background: Monkeypox is a global public health issue caused by the monkeypox virus (MPXV), a virus belonging to the Orthopoxvirus genus. As of October 28 2022, a total of 77,115 laboratory-confirmed cases and 3,610 probable cases, including 36 deaths, were reported, with 9,070 cases reported in Brazil, the second most affected country. The need to develop national technologies for the rapid diagnosis of emerging diseases for mass testing of the population is evident, as observed in the current SARS-CoV-2 pandemic. With that in mind, this article provides an overview of current methods, techniques, and their applications in the molecular detection of monkeypox. Methods: The relevant documents or papers covered in this study were selected by a search in international bibliographic databases. The search terms used in the databases were aimed at summarizing existing knowledge on molecular diagnostic methods, such as: monkeypox; MPX, MPXV, qPCR, PCR, PCR-ELISA, and Diagnosis and Detection searched separately or together using the Boolean operator AND either in the title or abstract. The searches took place in September 2022, and the corresponding articles were selected between 2012 and 2022. Results: We found 256 documents in total and twelve studies addressing the molecular diagnosis of monkeypox were classified as possible sources for this review. Conclusion: This paper presents new perspectives and an overview of current methods, technologies, and applications in the molecular diagnosis of monkeypox. It is evident there is a pressing need to develop national technologies for the rapid diagnosis of emerging diseases for mass testing of the population. It is extremely important to have national detection kits with greater diagnostic capacity to assist in developing effective public policies in countries affected by this disease.

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Molecular Methods for Diagnosis of Monkeypox: A Mini-review

Thomasi

Correa

Carmo

et al. 2024

CMM

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Background: Monkeypox is a global public health issue caused by the monkeypox virus (MPXV). As of October 28, 2022, a total of 77,115 laboratory-confirmed cases and 3,610 probable cases, including 36 deaths, were reported, with 9,070 cases reported in Brazil, the second most affected country. The need to develop national technologies for the rapid diagnosis of emerging diseases for mass testing of the population is evident, as observed in the SARS-CoV-2 pandemic. Objective: With that in mind, this article provides an overview of current methods, techniques, and their applications in the molecular detection of monkeypox, focusing the search on real-time polymerase chain reaction (qPCR), polymerase chain reaction (PCR), and polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA). Methods: The relevant documents or papers covered in this study were selected by a search in international bibliographic databases. The search terms used in the databases were aimed at summarizing existing knowledge on molecular diagnostic methods, such as monkeypox; MPX, MPXV, qPCR, PCR, PCR-ELISA, diagnosis and detection searched separately or together using the Boolean operator “AND” either in the title or abstract. The searches took place in September 2022, and the corresponding articles were selected between 2012 and 2022. Results: We found 256 documents in total and twelve studies addressing the molecular diagnosis of monkeypox were classified as possible sources for this review. Conclusion: It is evident there is a pressing need to develop national technologies for rapid diagnosis of emerging diseases for mass testing of the population. It is also extremely important to have national detection kits with greater diagnostic capacity to assist in developing effective public policies in countries affected by this disease.

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Evolução da técnica de PCR: sua contribuição no diagnóstico da infecção por HPV

Cited by 4 publications

References 20 publications

Health in focus: HPV

Health in focus: HPV

Molecular methods for diagnosis of monkeypox: A mini-review

Molecular Methods for Diagnosis of Monkeypox: A Mini-review

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