Evidence That Sry Is Expressed in Pre-Sertoli Cells and Sertoli and Granulosa Cells Have a Common Precursor

Albrecht, Kenneth H.; Eicher, Eva M.

doi:10.1006/dbio.2001.0438

Cited by 345 publications

(311 citation statements)

References 34 publications

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“…In rainbow trout, these cells are clearly in a different location from those expressing sox9a2 and amh, which are expressed in the vicinity of germ cells both in female and male gonads. In the mouse, granulosa and Sertoli cells are considered to develop from a common precursor (Albrecht and Eicher, 2001) and Amh and Sox9 are classic markers of Sertoli cells. In trout, the localization of amh and sox9a2 expression suggests that these genes are also expressed in granulosa cell precursors, and then cyp19a1 would be expressed in another somatic cell type.…”

Section: Figmentioning

confidence: 99%

Characterization of early molecular sex differentiation in rainbow trout, Oncorhynchus mykiss

Vizziano

Randuineau²,

Báron³

et al. 2007

Developmental Dynamics

180

171

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Early differentiation in rainbow trout gonads was investigated by expression profiling and in situ hybridization (ISH). Expression of cyp19a1 and fst in females and sox9a1 in males were sexually dimorphic between 32 to 35 days post-fertilization (dpf). After 35 dpf, the differentiation proceeded with sexually dimorphic profiles for sox9a2, dmrt1, cyp11b2.1, amh in males and foxl2a, foxl2b, hsd3b1, inha in females. cyp17a1, cyp11a1, star, nr5a1b increased only after 40 dpf in both sexes with a slightly higher expression in females. cyp19a1 expression was localized in a cluster of somatic cells in the ventral side of female gonads, and sox9a2 and amh in somatic cells surrounding the germ cells, at 28 dpf and thereafter, both in male and female gonads. cyp11b2.1, cyp17a1, and cyp11a1 expressions were only detected in scattered somatic cells in males after 46 dpf. This confirms the early implication of cyp19a1 in trout ovarian differentiation and suggests that early testicular differentiation does not need androgen production. Developmental Dynamics

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Section: Figmentioning

confidence: 99%

Characterization of early molecular sex differentiation in rainbow trout, Oncorhynchus mykiss

Vizziano

Randuineau²,

Báron³

et al. 2007

Developmental Dynamics

180

171

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“…SRY is expressed in XY genital ridges between approximately E10.5 and E13 with peak RNA expression at E11.5, al- though little information is available on protein levels. Recent studies showed that SRY was expressed in the central region of genital ridges and then became localized nearer to the gonadal surface and cranial and caudal poles (Albrecht and Eicher, 2001;Bullejos and Koopman, 2001). A cell-tracing experiment using Sry-EGFP transgenic mice demonstrated that SRY-positive cells were closely associated with and sometimes partially surrounded each germ cell at E11.…”

Section: Expression Of Dmy Before and During Testicular Differentiationmentioning

confidence: 99%

“…Recent detailed cell-tracing experiment using Sry-EGFP transgenic mice demonstrated that Sry was expressed in pre-Sertoli cells (Albrecht and Eicher, 2001). In medaka, PGCs are surrounded by the somatic cells derived from lateral plate mesoderm during PGC migration and contribute to gonadal formation (Hamaguchi, 1982;this study).…”

Section: Dmy Positive Cells Are Pre-sertoli Cellsmentioning

confidence: 99%

Two DM domain genes, DMY and DMRT1, involved in testicular differentiation and development in the medaka, Oryzias latipes

Kobayashi

Matsuda

Kajiura-Kobayashi

et al. 2004

Developmental Dynamics

288

239

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The recent discovery of the DMY gene (DM domain gene on Y chromosome and one of the DMRT1 family genes) as a key determinant of male development in the medaka (Oryzias latipes) has led to its designation as the prime candidate gene for sex-determination in this species. This study focused on the sites and pattern of expression of DMY and DMRT1 genes during gonadal differentiation of medaka to further determine their roles in testis development. DMY mRNA and protein are expressed specifically in the somatic cells surrounding primordial germ cells (PGCs) in the early gonadal primordium, before morphological sex differences are seen. However, somatic cells surrounding PGCs never express DMY during the early migratory period. Expression of DMY persists in Sertoli cell lineage cells, from PGC-supporting cells to Sertoli cells, indicating that only DMY-positive cells enclose PGCs during mitotic arrest after hatching. DMRT1 is expressed in spermatogonium-supporting cells after testicular differentiation (20 -30 days after hatching), and its expression is much higher than that of DMY in mature testes. In XX sex-reversed testes, DMRT1 is expressed in the Sertoli cell lineage, similar to the expression of DMY in XY testes. These results suggest strongly that DMY regulates PGC proliferation and differentiation sex-specifically during early gonadal differentiation of XY individuals and that DMRT1 regulates spermatogonial differentiation. Developmental Dynamics 231:518 -526, 2004.

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“…In mammals, Sertoli cell precursors express the male sex-determining gene Sry, which appears to act through this cell type to divert the gonad from the ovarian to the testis pathway (Burgoyne and Palmer, 1993;Albrecht and Eicher, 2001). In mice, one of the first differences between the sexes is an increase in proliferation of Sertoli cells (Schmahl et al, 2000).…”

Section: Proliferation Of Sertoli Precursors May Have a Role In Vertementioning

confidence: 99%

“…Sertoli cells have critical roles in early testis development. Not only are they support cells for germ cells and essential components of testis cords, but there is also evidence that Sertoli cells express Sry and direct the morphogenesis of the entire testis (Burgoyne and Palmer, 1993;Albrecht and Eicher, 2001). In alligators, an early proliferation in Sertoli cell precursors is one of the first morphological differences between the sexes (Smith and Joss, 1994), indicating that proliferation in this critical cell type may be a conserved mechanism in vertebrate testis organogenesis.…”

Section: Introductionmentioning

confidence: 99%

Colocalization of WT1 and cell proliferation reveals conserved mechanisms in temperature‐dependent sex determination

Schmahl

Yao

Pierucci-Alves

et al. 2003

Genesis

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SummaryDuring vertebrate development the gonad has two possible fates, the testis or the ovary. The choice between these fates is made by a variety of sex-determining mechanisms, from the sexdetermining gene on the Y chromosome (Sry) in mammals, to nongenetic temperature-dependent systems in many reptiles. Despite the differences in the mechanisms at the top of the sexdetermining cascade, the resulting morphology and many genes involved in early testis and ovarian development are common to most vertebrates, leading to the hypothesis that the underlying processes of sex determination are conserved. In this study, we examined the early steps of gonad development in the red-eared slider turtle (Trachemys scripta), a species that uses the temperature of egg incubation to determine sex. A dramatic increase in cell proliferation was observed in the male gonad during the earliest stages of sex determination. Using the localization of Wilms' Tumor suppressor 1 (WT1), we determined that this proliferation increase occurred in a population that contained pre-Sertoli cells. The proliferation of pre-Sertoli cells has been documented during sex determination in both mice and alligators, suggesting that proliferation of this cell type has an important role in vertebrate testis organogenesis and the determination of male fate.

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Evidence That Sry Is Expressed in Pre-Sertoli Cells and Sertoli and Granulosa Cells Have a Common Precursor

Cited by 345 publications

References 34 publications

Characterization of early molecular sex differentiation in rainbow trout, Oncorhynchus mykiss

Characterization of early molecular sex differentiation in rainbow trout, Oncorhynchus mykiss

Two DM domain genes, DMY and DMRT1, involved in testicular differentiation and development in the medaka, Oryzias latipes

Colocalization of WT1 and cell proliferation reveals conserved mechanisms in temperature‐dependent sex determination

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