1999
DOI: 10.1038/sj.bjp.0702828
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Evidence that mast cell degranulation, histamine and tumour necrosis factor α release occur in LPS‐induced plasma leakage in rat skin

Abstract: 1 In the present study we investigated the role of mast cells during in¯ammation in rat skin. As the release of several pro-in¯ammatory mediators, such as histamine and tumour necrosis factor a (TNFa), occurs following mast cell activation we studied whether mast cell degranulation and the release of both histamine (H) and TNFa occurred in a model of lipopolysaccharide (LPS)-induced plasma leakage in rat skin. 2 Plasma leakage in the rat skin was measured over a period of 2 h as the local accumulation of intra… Show more

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Cited by 40 publications
(33 citation statements)
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“…Because LT induces a cytokine-independent, shock-like death in mice and rats, we hypothesized that anaphylactic shock-like events due to mast cell degranulation or release of histamine from other cells could be the cause of LT-mediated vascular leakage and shock (26,39). To test this hypothesis, we utilized the drug ketotifen, which inhibits mast cell, basophil, and eosinophil degranulation and interferes with histamine function (14), protecting against shock (2, 35) and inhibiting LPS-mediated leakage by preventing mast cell degranulation, in a Miles assay (18,19). Ketotifen or saline (control) was introduced to mice systemically by i.p.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…Because LT induces a cytokine-independent, shock-like death in mice and rats, we hypothesized that anaphylactic shock-like events due to mast cell degranulation or release of histamine from other cells could be the cause of LT-mediated vascular leakage and shock (26,39). To test this hypothesis, we utilized the drug ketotifen, which inhibits mast cell, basophil, and eosinophil degranulation and interferes with histamine function (14), protecting against shock (2, 35) and inhibiting LPS-mediated leakage by preventing mast cell degranulation, in a Miles assay (18,19). Ketotifen or saline (control) was introduced to mice systemically by i.p.…”
Section: Resultsmentioning
confidence: 99%
“…Fischer 344 rats were purchased from Taconic Farms (Germantown, NY) and used at weights of 150 to 180 g. Rats were injected intravenously (i.v.) in the tail vein with 12 g LT, which has been shown by our studies to result in death within 60 min (unpublished observations) with or without 250 g of the mast cell stabilizer drug ketotifen (18,19), and monitored for the exact time to death.…”
mentioning
confidence: 99%
“…The endotoxin syndrome is associated with a release of in¯ammatory mediators including eicosanoids, cytokines, platelet-activating factor (PAF) and nitric oxide (Liao, 1996;Karima et al, 1999). Subcutaneous injection of LPS on the back of mice/rats induces a plasma leakage at the site of injection, which has been used as an in vivo model of in¯ammation (Fujii et al, 1996a, b;Iuvone et al, 1999). This LPS-induced increase in vascular permeability is mediated by TNF-a, IL-1a, histamine, nitric oxide, PAF and prostaglandins in the mouse (Fujii et al, 1996a, b;Wada et al, 2000) and by histamine, nitric oxide and TNF-a in the rat (Iuvone et al, 1999).…”
Section: Introductionmentioning
confidence: 99%
“…as tumor necrosis factor-alpha (TNF-α) and histamine, with the mast cell degranulation [14] . Therefore, many investigators have made much effort to abolish or reduce the LPS-elicited injury.…”
Section: Compound Danshen Injection Improves Endotoxin-induced Microcmentioning
confidence: 99%