Compound Danshen injection improves endotoxin-induced microcirculatory disturbance in rat mesentery

Abstract

stained with Monastral blue B, rolling and adhesion of leukocytes, production of oxygen radical in venular wall, albumin efflux and enhanced mast cell degranulation in vivo , all of which, except for the leukocyte rolling, were attenuated by the treatment with compound Danshen injection. Experiments performed in vitro further revealed that the expression of CD11b/CD18 and the production of oxygen free radical in neutrophils, and the expression of ICAM-1 in HUVECs were increased by exposure to LPS, and they were attenuated by compound Danshen injection. CONCLUSION: These results suggest that compoundDanshen injection is an efficient drug with multi-targeting potential for improving the microcirculatory disturbance. INTRODUCTIONIt is now well-recognized that stimulation with lipopolysaccharide (LPS) leads to a number of responses in microcirculation, such as endothelial cell damage, an increase in rolling, adhesion and emigration of leukocytes in post-capillary venules, enhanced oxygenfree radical production, mast cell degranulation and protein efflux [1][2][3][4][5][6] . Furthermore, it is demonstrated that LPS stimulation evokes an increase in the expression of adhesion molecules, including L-selectin [7] and CD11b/CD18 in leukocytes, E-selectin and intercellular adhesion molecule 1 (ICAM-1) in endothelial cells [7][8][9][10][11][12][13] . In addition, LPS stimulation can cause the release of proinflammatory mediators and vasoactive substances, such Abstract AIM: To investigate the effect of compound Danshen injection on lipopolysaccharide (LPS)-induced rat mesenteric microcirculatory dysfunctions and the underlying possible mechanism by an inverted intravital microscope and high-speed video camera system. METHODS:LPS was continuously infused through the jugular artery of male Wistar rats at the dose of 2 mg/kg per hour. Changes in mesenteric microcirculation, such as diameters of arterioles and venules, velocity of RBCs in venules, leukocyte rolling, adhesion and emigration, free radicals released from post-capillary venules, FITCalbumin leakage and mast cell degranulation, were observed through an inverted intravital microscope assisted with CCD camera and SIT camera. Meanwhile, the expression of adhesion molecules CD11b/CD18 and the production of free radical in neutrophils, and the expression of intercellular adhesion molecule 1 (ICAM-1) in human umbilical vein endothelial cells (HUVECs) were quantified by flow cytometry (FACS) in vitro . RESULTS:The continuous infusion with LPS resulted in a number of responses in microcirculation, including a significant increase in the positive region of venule

Ginsenoside Rb1 ameliorates lipopolysaccharide-induced albumin leakage from rat mesenteric venules by intervening in both trans- and paracellular pathway

“…In the present study, we provide compelling experimental evidence supporting the efficacy of Rb1 therapy for mesentery albumin leakage induced by LPS. Previous studies in our laboratory have demonstrated that LPS intravenous infusion at a concentration of 5 mg/kg effectively evoked hyperpermeability in microvessels (13,44). Consistent with our previous studies, with the use of inverted intravital microscopy, the present study revealed that FITC-labeled albumin leaked markedly out of mesenteric microvessel 30 min after LPS infusion and increased linearly with time.…”

“…After being anesthetized with urethane (2 g/kg body wt im), the left femoral vein and left jugular vein of the rat were cannulated. In the LPS group, LPS solution in saline was infused (5 mg·kg Ϫ1 ·h Ϫ1 ) for 90 min via the left femoral vein, as previously described (13,44). The vehicle, instead of LPS solution, was administrated in Sham and Rb1 alone (Rb1) groups.…”

The stability investigation of compound Danshen injection (a traditional medicine) with a new high-performance liquid chromatography method

“…[17] It mainly contains water-soluble and bioactive phenolic acids, such as propanoid acid (PA), protocatechualdehyde (PHA), salvianolic acid B (SAB), salvianolic acid A (SAA), and rosmarinic acid (RA). [18] Although some researchers have indicated that Danshen injection possess low acute and sub-chronic toxicity,[19] more and more ADR cases induced by it are reported in the recent years. [20]…”

Dual effects of lipophilic extract of Salvia miltiorrhiza (Danshen) on catecholamine secretion in cultured bovine adrenal medullary cells

“…Chemical compounds from Salvia miltiorrhiza can be classified into two major categories: hydrophilic compounds and lipophilic diterpenoid quinines (LDQ). Both hydrophilic and lipophilic compounds of Danshen have multiple pharmacological activities, such as improving the microcirculatory disturbance, protecting against cardiotoxicity induced by doxorubicin, inhibiting the proliferation of vascular smooth muscle cells, anti-inflammatory, anti-platelet, anti-oxidant and vasorelaxation (Seon-il et al, 2003;Wang et al, 2005;Han et al, 2007;Jiang et al, 2009;Wu et al, 2009).…”