Evidence that a 210,000-molecular-weight glycoprotein (GP 210) serves as a platelet Fc receptor.

Stricker, Raphael B.; Reyes, P; Corash, Laurence; Shuman, Marc A.

doi:10.1172/jci112993

Cited by 16 publications

(1 citation statement)

References 19 publications

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“…It is possible that IgG and C3 receptors on the platelet mem brane [18,19] became activated during storage, and that plasma IgG and C3 attached to these receptors, followed by internalization of IgG and C3. Further studies are in pro gress to delineate this mechanism more fully.…”

Section: Discussionmentioning

confidence: 99%

Storage of Platelets in Earth Orbit. Effect of Gravity and the Formulation of Plastic Storage Containers on Platelet IgG and C3

Szymanski¹,

Odgren²,

Teno³

et al. 1989

Vox Sanguinis

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We investigated the effects of gravitational force and three different plastic formulations of the storage bags on the quality of stored platelets by measuring the changes in platelet-associated immunoglobulin G (IgG) and two antigenic markers of the third component of human complement (C3), C3d and C3c. Pooled platelets were stored in parallel at microgravity (MG) and on the ground (lg) using three different plastic polymers: (1) polyvinylchloride (PVC) plasticized with di-2-ethylhexyl phthalate (DEHP); (2) PVC plasticized with trioctyl trimellitate (TOTM), and (3) unplasticized polyolefin (PO). The IgG and C3 were quantified by an automated antiglobulin consumption test in freeze/thaw disrupted platelets (total IgG or C3). The baseline values for platelet associated IgG and C3, measured after 2.5 days of storage at 1g just prior to the launch, fell within the normal range. Following an additional 6.5 day storage, platelets stored at MG had accumulated significantly less C3d than those stored at lg, suggesting that MG storage was beneficial. Specific plastic formulations also exerted a significant effect on the accumulation of these immunoproteins, the effect being particularly pronounced at MG. The smallest increases of IgG and C3 were seen in platelets stored in TOTM and the largest in those stored in DEHP. It is possible that further studies at MG would permit a clear characterization of the effects of other independent storage variables.

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Section: Discussionmentioning

confidence: 99%

Storage of Platelets in Earth Orbit. Effect of Gravity and the Formulation of Plastic Storage Containers on Platelet IgG and C3

Szymanski¹,

Odgren²,

Teno³

et al. 1989

Vox Sanguinis

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Immunohistochemical and functional studies of glycoprotein 60 (gp60) in platelets

et al. 1992

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We showed by immunofluorescence, immunoelectron microscopy and Western blot analysis that the plasma glycoprotein (gp60), an Fc gamma binding protein which inhibits complement-mediated prevention of immune precipitation, is present in platelets. The gp60 content of platelets in normal individuals and patients with rheumatoid arthritis was similar (mean 0.028 and 0.024 fg/platelet respectively). Immunoelectron microscopic studies showed that gp60 was present in the cytoplasm and the surface connecting structures but not in the alpha granules, dense granules or lysosomes. Using this technique gp60 was also found on platelet membranes, an observation which was confirmed by immunofluorescence. Activation of platelets with thrombin, calcium ionophore, and immune complexes (IC) resulted in the release of the contents of the alpha granules (beta-thromboglobulin), dense granules (5-hydroxytryptamine) and lysosomes (beta-glucuronidase) but did not induce gp60 secretion. The inability of Fab anti-gp60 to inhibit IC-mediated platelet aggregation and of F(ab')2 anti-gp60 to produce platelet aggregation suggested that IC-mediated platelet aggregation did not occur as a result of the interaction of IC with platelet gp60. However, as the preincubation of IC with purified gp60 produced dose-dependent inhibition of the ability of IC to aggregate platelets it is possible that fluid-phase plasma gp60 modulates the interaction of IC with platelets.

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Interaction between the IgG-Fc fragment and subfragments and the IgG-Fc receptor on human platelets

Endresen¹

1989

Thrombosis Research

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Evidence that a 210,000-molecular-weight glycoprotein (GP 210) serves as a platelet Fc receptor.

Abstract: We previously identified a 210,000-mol-wt platelet glycoprotein (GP 210)

Cited by 16 publications

References 19 publications

Storage of Platelets in Earth Orbit. Effect of Gravity and the Formulation of Plastic Storage Containers on Platelet IgG and C3

Storage of Platelets in Earth Orbit. Effect of Gravity and the Formulation of Plastic Storage Containers on Platelet IgG and C3

Immunohistochemical and functional studies of glycoprotein 60 (gp60) in platelets

Interaction between the IgG-Fc fragment and subfragments and the IgG-Fc receptor on human platelets

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