Evidence of Vertical Transmission of Dengue Virus in Two Endemic Localities in the State of Oaxaca, Mexico

Günther, Jeannette; Martínez-Muñoz, Jorge Pascual; Pérez-Ishiwara, David Guillermo; Salas-Benito, Juan Santiago

doi:10.1159/000107272

Cited by 63 publications

(53 citation statements)

References 40 publications

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“…Viruses such as dengue virus and Chikungunya virus can be vertically transmitted from an infected adult female to its offspring. Dengue virus can spread vertically under both natural (66)(67)(68)(69) and laboratory conditions (70)(71)(72). Furthermore, transovarially infected female mosquitoes can transmit dengue virus orally (73).…”

Section: Figmentioning

confidence: 99%

Wolbachia-Mediated Antiviral Protection in Drosophila Larvae and Adults following Oral Infection

Stevanovic

Arnold

Johnson

2015

Appl Environ Microbiol

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Understanding viral dynamics in arthropods is of great importance when designing models to describe how viral spread can influence arthropod populations. The endosymbiotic bacterium Wolbachia spp., which is present in up to 40% of all insect species, has the ability to alter viral dynamics in both Drosophila spp. and mosquitoes, a feature that in mosquitoes may be utilized to limit spread of important arboviruses. To understand the potential effect of Wolbachia on viral dynamics in nature, it is important to consider the impact of natural routes of virus infection on Wolbachia antiviral effects. Using adult Drosophila strains, we show here that Drosophila-Wolbachia associations that have previously been shown to confer antiviral protection following systemic viral infection also confer protection against virus-induced mortality following oral exposure to Drosophila C virus in adults. Interestingly, a different pattern was observed when the same fly lines were challenged with the virus when still larvae. Analysis of the four Drosophila-Wolbachia associations that were protective in adults indicated that only the w1118-wMelPop association conferred protection in larvae following oral delivery of the virus. Analysis of Wolbachia density using quantitative PCR (qPCR) showed that a high Wolbachia density was congruent with antiviral protection in both adults and larvae. This study indicates that Wolbachia-mediated protection may vary between larval and adult stages of a given Wolbachia-host combination and that the variations in susceptibility by life stage correspond with Wolbachia density. The differences in the outcome of virus infection are likely to influence viral dynamics in Wolbachia-infected insect populations in nature and could also have important implications for the transmission of arboviruses in mosquito populations.A rthropods harbor a wide range of viruses that can be transmitted between individuals or populations of the same species or can bridge the interspecies gap to infect plants or other animals. The outcome of viral infections can be modulated by tripartite interactions between arthropods, viruses, and bacteria (1). One such interaction is the tripartite interaction between insects, viruses, and the endosymbyotic bacterium Wolbachia pipientis.Wolbachia spp. have gained much attention due to the antiviral effects they confer to their host. The impact of Wolbachia spp. on virus infection was first described in the Drosophila melanogaster host, where it was shown to protect against mortality induced by diverse viruses, including Drosophila C virus (DCV), cricket paralysis virus, and Flock House virus (2, 3). Since that discovery, Wolbachia-mediated antiviral effects have been demonstrated in a number of insect hosts and are being investigated as a way of limiting spread of arboviruses (reviewed in references 1, 4, 5, and 6). Notably, Wolbachia-mediated antiviral effects have been demonstrated in adult mosquitoes artificially infected with Wolbachia; in mosquitoes, Wolbachia can interfere with accu...

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Section: Figmentioning

confidence: 99%

Wolbachia-Mediated Antiviral Protection in Drosophila Larvae and Adults following Oral Infection

Stevanovic

Arnold

Johnson

2015

Appl Environ Microbiol

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“…The detection and the serotyping of the virus was performed with the RT-PCR Access kit ® (Promega) using the nested RT-PCR method reported by Seah 16 and modified by Günther. 17 Briefly, the RT reaction was performed at 48 • C for 45 min followed by 35 PCR cycles of 94 • C for 30 s, 55 • C for 60 s and 68 • C for 60 s with a final cycle of 68 • C for 10 min using DV1 sense and DV1 anti-sense primers. The positive samples were subjected to 30 additional PCR cycles using specific primers for each DEN virus serotype (DSP1-DSP4) in the same conditions described above.…”

Section: Den Virus Serotypingmentioning

confidence: 99%

Distribution of dengue cases in the state of Oaxaca, Mexico, during the period 2004–2006

Günther

Ramírez-Palacio

Pérez-Ishiwara

et al. 2009

Journal of Clinical Virology

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“…aegypti mosquitoes was recorded in two endemic localities. Although the presence of DENV in larvae could not be demonstrated, DENV-2, -3 and -4 serotypes were detected in four out of 43 pools of in-cage born mosquitoes (Günther et al, 2007). In Acapulco, Guerrero, only two (0.9%) of 226 pools of Ae.…”

Section: Vertical Transmissionmentioning

confidence: 87%

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2018

Safety Assessment of Transgenic Organisms in the Environment, Volume 8

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This work is published under the responsibility of the Secretary-General of the OECD. The opinions expressed and arguments employed herein do not necessarily reflect the official views of OECD member countries.This document, as well as any data and any map included herein, are without prejudice to the status of or sovereignty over any territory, to the delimitation of international frontiers and boundaries and to the name of any territory, city or area.Please cite this publication as: OECD (2018) The statistical data for Israel are supplied by and under the responsibility of the relevant Israeli authorities. The use of such data by the OECD is without prejudice to the status of the Golan Heights, East Jerusalem and Israeli settlements in the West Bank under the terms of international law.Photo credits: Cover © Maks Ershov.Corrigenda to OECD publications may be found on line at: www.oecd.org/about/publishing/corrigenda.htm. © OECD 2018You can copy, download or print OECD content for your own use, and you can include excerpts from OECD publications, databases and multimedia products in your own documents, presentations, blogs, websites and teaching materials, provided that suitable acknowledgment of the source and copyright owner(s) is given. All requests for public or commercial use and translation rights should be submitted to rights@oecd.org. Requests for permission to photocopy portions of this material for public or commercial use shall be addressed directly to the Copyright Clearance Center (CCC) at info@copyright.com or the Centre francais d'exploitation du droit de copie (CFC) at contact@cfcopies.com. FOREWORD │ 3SAFETY ASSESSMENTOF TRANSGENIC ORGANISMSIN THE ENVIRONMENT, VOLUME 8 © OECD 2018 ForewordModern biotechnologies are applied to plants species (crops, flowers, trees), animals and micro-organisms. The safety of the resulting transgenic organisms when released in the environment for their use in agriculture, forestry, the food and feed industry or for other applications represents a challenging issue. Genetically engineered products are rigorously assessed by their developers during their elaboration, and by governments when ready for release, to ensure high safety standards. This remains essential with new biotechnology developments using insects to fight against disease outbreaks: engineered mosquitoes need to be evaluated through a scientifically sound approach to risk/safety assessment that will inform biosafety regulators and support the decision concerning the release of these novel organisms in the environment.The OECD offers a long-standing recognised expertise in biosafety and contributes to facilitating an harmonised approach. The OECD's Working Group on Harmonisation of Regulatory Oversight in Biotechnology (WG-HROB) was established in 1995. The WG-HROB gathers national authorities responsible for the environmental risk/safety assessment of products of modern biotechnology in OECD countries and other economies. International organisations and experts involved in biosafety activities are a...

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Evidence of Vertical Transmission of Dengue Virus in Two Endemic Localities in the State of Oaxaca, Mexico

Cited by 63 publications

References 40 publications

Wolbachia-Mediated Antiviral Protection in Drosophila Larvae and Adults following Oral Infection

Wolbachia-Mediated Antiviral Protection in Drosophila Larvae and Adults following Oral Infection

Distribution of dengue cases in the state of Oaxaca, Mexico, during the period 2004–2006

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