Evidence of superclones in Australian cotton aphid <i>Aphis gossypii</i>
 Glover (Aphididae: Hemiptera)

Chen, Yizhou; Vanlerberghe‐Masutti, Flavie; Wilson, Lewis; Barchia, I.; McLoon, Martin O; Smith, Tanya; Herron, Grant A

doi:10.1002/ps.3455

Cited by 33 publications

(19 citation statements)

References 28 publications

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“…In fact, a recent genetic structure study of A. gossypii in Australia showed weak host plant specialisation and many plant species could serve as refuge plants for A. gossypii. (Chen et al 2013). However, it is also likely that the kdr resistance detected in A.…”

Section: < Table 2 Discussionmentioning

confidence: 98%

A TaqMan qPCR method for detecting kdr resistance in Aphis gossypii demonstrates improved sensitivity compared to conventional PCR–RFLP

et al. 2015

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Cotton aphid, Aphis gossypii Glover, has emerged as a prominent pest in Australian cotton production, monitoring pesticide resistance including pyrethroids in field populations is crucial for its sustainable management. We examined the distribution of kdr resistance in 35 field collected A. gossypii populations and used a TaqMan qPCR assays with pooled samples. The study demonstrated proof of concept that pooled insect qPCR methodology provided effective detection with better sensitivity than individual PCR-RFLP genotyping techniques for the kdr resistance allele. The practical outcome is that routine resistance monitoring can examine more sites while increasing the likelihood of detecting incipient resistance at those sites. More importantly, the method is adaptable to any genetically caused resistance and so not limited to A. gossypii or even insect control. It cannot be overstressed that the ability to detected resistance at very low frequencies is critical to all sustainable resistance management. Early detection of resistance provides critical time for the modification of chemical use prior to potential insecticide control failure.

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Section: < Table 2 Discussionmentioning

confidence: 98%

A TaqMan qPCR method for detecting kdr resistance in Aphis gossypii demonstrates improved sensitivity compared to conventional PCR–RFLP

et al. 2015

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“…Organisms with clonal reproduction may exhibit reduced genetic diversity within populations, as better-adapted clonal genotypes expand and dominate available resources [6]. In aphids, the concept of “superclones” emerged [7] when a few asexual genotypes of the same species were able to colonize a wide geographical or ecological range of habitats [8]–[11]. The capacity of these populations to adapt to different conditions could be the result of a preadaptation capacity for phenotypic plasticity rather than local selection acting on genetic diversity [12], [13].…”

Section: Introductionmentioning

confidence: 99%

Low Genetic Diversity in Melanaphis sacchari Aphid Populations at the Worldwide Scale

et al. 2014

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Numerous studies have examined the genetic diversity and genetic structure of invading species, with contrasting results concerning the relative roles of genetic diversity and phenotypic plasticity in the success of introduced populations. Increasing evidence shows that asexual lineages of aphids are able to occupy a wide geographical and ecological range of habitats despite low genetic diversity. The anholocyclic aphid Melanaphis sacchari is a pest of sugarcane and sorghum which originated in the old world, was introduced into the Americas, and is now distributed worldwide. Our purpose was to assess the genetic diversity and structuring of populations of this species according to host and locality. We used 10 microsatellite markers to genotype 1333 individuals (57 samples, 42 localities, 15 countries) collected mainly on sugarcane or sorghum. Five multilocus lineages (MLL) were defined, grouping multilocus genotypes (MLG) differing by only a few mutations or scoring errors. Analysis of a 658 bp sequence of mitochondrial COI gene on 96 individuals revealed five haplotypes, with a mean divergence of only 0.19 %. The distribution of MLL appeared to be strongly influenced by geography but not by host plant. Each of the five MLL grouped individuals from (A) Africa, (B) Australia, (C) South America, the Caribbean and the Indian Ocean including East Africa, (D) USA, and (E) China. The MLL A and C, with a wide geographic distribution, matched the definition of superclone. Among aphids, M. sacchari has one of the lowest known rates of genetic diversity for such a wide geographical distribution.

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“…The mode of reproduction is another factor influencing genetic diversity. Aphids reproducing by parthenogenesis have low genetic diversity on local and worldwide scales (Carletto et al, 2009;Chen et al, 2013). Additionally, the average transition (ti)/transversion bias based on the point or base substitution was R = 1.69 (Table 2).…”

Section: Resultsmentioning

confidence: 99%

DNA barcoding of black cherry aphid Myzus cerasi (Fabricus, 1775) (Hemiptera: Aphididae) populations collected from Prunus avium and Prunus cerasus

İnal¹,

Kandemi̇r²

2020

Turk J Zool

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Sequences of the mitochondrial cytochrome oxidase I (COI) gene of one Greek and 25 Turkish Myzus cerasi (Fabricus) (Hemiptera: Aphididae) populations collected from Prunus avium and Prunus cerasus were analyzed. The partial coding region of COI studied is 605 bp for all the populations, from which 565 nucleotides were conserved, 40 were variable, 37 were singleton, and 3 were parsimony-informative. Four haplotypes were identified based on nucleotide substitutions and the mean of intraspecific divergence was calculated to be 0.3%. Phylogenetic trees were constructed using maximum likelihood, neighbor joining, and minimum evolution. Myzus persicae (Sulzer) and Myzus borealis Ossiannilson were included as outgroups. The population of M. cerasi from Isparta diverged from the rest of the groups and formed a clade (Haplotype B) with Myzus borealis. The rest of the haplotype diversity included Haplotype A and Haplotype C with individuals characterized as Myzus cerasi pruniavium and Haplotype D with Myzus cerasi cerasi. M. cerasi diverged into two subspecies and it must be reevaluated whether this pest is monophagous or oligophagous in terms of plant type dependence. Further studies will be required for a complete barcoding and host-specific status of black cherry aphid.

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Evidence of superclones in Australian cotton aphid Aphis gossypii Glover (Aphididae: Hemiptera)

Abstract: The study demonstrated that any strategy to control A. gossypii and manage pirimicarb resistance should target A. gossypii strains of all MLG types residing on any plant species and not just cotton

Cited by 33 publications

References 28 publications

A TaqMan qPCR method for detecting kdr resistance in Aphis gossypii demonstrates improved sensitivity compared to conventional PCR–RFLP

A TaqMan qPCR method for detecting kdr resistance in Aphis gossypii demonstrates improved sensitivity compared to conventional PCR–RFLP

Low Genetic Diversity in Melanaphis sacchari Aphid Populations at the Worldwide Scale

DNA barcoding of black cherry aphid Myzus cerasi (Fabricus, 1775) (Hemiptera: Aphididae) populations collected from Prunus avium and Prunus cerasus

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