Evidence of Insecticide Resistance to Pyrethroids and Bendiocarb in<i> Anopheles funestus</i> from Tsararano, Marovoay District, Madagascar

Rakotondranaivo, Tsiriniaina; Randriamanarivo, Solohery Fanomezana; Tanjona, Mihajarilala Rakotoniaina; Vigan-Womas, Inès; Randrianarivelojosia, Milijaona; Ndiath, Mamadou Ousmane

doi:10.1155/2018/5806179

Cited by 8 publications

(7 citation statements)

References 29 publications

(45 reference statements)

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“…Indeed, previous data from sentinel sites of the National Malaria Control Programme across the country showed low resistance of species of the An. gambiae complex to bendiocarb [6,7]; resistance of species of the complex to the carbamate class of insecticide has been reported elsewhere in Africa in many studies [10,13,40,41].…”

Section: Susceptibility To Carbamatesmentioning

confidence: 99%

Acetylcholinesterase (ace-1R) target site mutation G119S and resistance to carbamates in Anopheles gambiae (sensu lato) populations from Mali

et al. 2020

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Background: The long-lasting insecticidal nets (LLINs) and indoor residual spraying of insecticide (IRS) are major malaria vector control strategies in Mali. The success of control strategies depends on a better understanding of the status of malaria vectors with respect to the insecticides used. In this study we evaluate the level of resistance of Anopheles gambiae (sensu lato) to bendiocarb and the molecular mechanism that underlies it. Methods: Larvae of An. gambiae (s.l.) were collected from breeding habitats encountered in the three study sites and bioassayed with bendiocarb. The ace-1 target site substitution G119S was genotyped using a TaqMan assay. Results: The three species of the An. gambiae complex in Mali, i.e. An. arabiensis, An. coluzzii and An. gambiae (s.s.) were found in sympatry in the three surveyed localities with different frequencies. We observed a resistance and suspicious resistance of the three species to bendiocarb with a mortality rate ranging from 37% to 86%. The allelic frequency of the G119S mutation was higher in An. gambiae (s.s.) compared to the other two species; 42.86%, 25.61% and 16.67% respectively in Dangassa, Koula, and Karadié. The allelic frequency of G119S in An. coluzzii ranged from 4.5% to 8.33% and from 1.43% to 21.15% for An. arabiensis. After exposure to bendiocarb, the G119S mutation was found only in survivors. The survival of Anopheles gambiae (s.l) populations from the three surveyed localities was associated with the presence of the mutation. Conclusions: The study highlights the implication of G119S mutation in bendiocarb resistance in An. gambiae (s.s.), An. arabiensis and An. coluzzii populations from the three surveyed localities.

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Section: Susceptibility To Carbamatesmentioning

confidence: 99%

Acetylcholinesterase (ace-1R) target site mutation G119S and resistance to carbamates in Anopheles gambiae (sensu lato) populations from Mali

et al. 2020

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“…aegypti and Cx. quinquefasciatus resistance to PBO-synergized insecticides, while resistant Anopheles also developed GST and A296S-RDL dieldrin resistance mechanisms [31,37,42,49,50,54]. In this study, we found that both P450 and COE enzyme activities in field Cx.…”

Section: Discussionmentioning

confidence: 56%

“…We should note that it is unlikely that African Anopheles mosquitoes were exposed to PBO or PBO-synergized insecticide products before large-scale rollout of PBO-LLINs. As discussed earlier, the reduced PBO-LLIN killing efficacy is likely due to multiple resistance mechanisms [49,50,53,54]; i.e., adding PBO to LLIN may not be enough to combat Anopheles mosquitoes' resistance to pyrethroids. This study has some limitations.…”

Section: Discussionmentioning

confidence: 99%

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Emerging mosquito resistance to piperonyl butoxide-synergized pyrethroid insecticide and its mechanism

Zhou

Jeang

et al. 2021

Preprint

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Piperonyl butoxide (PBO)-synergized pyrethroid products are widely available for the control of pyrethroid-resistant mosquitoes. To date, no study has formally examined mosquito resistance to PBO-synergized insecticides. We used Culex quinquefasciatus as a model mosquito examined the insecticide resistance mechanisms of mosquitoes to PBO-synergized pyrethroid using modified World Health Organization tube bioassays and biochemical analysis of metabolic enzyme expressions prior- and post-PBO exposure. We measured mosquito mortalities and metabolic enzyme expressions in mosquitoes with/without pre-exposure to different PBO concentrations and exposure durations. We found that field Culex quinquefasciatus mosquitoes were resistant to all insecticides tested, including PBO-synergized pyrethroids (mortality ranged from 3.7±4.7% to 66.7±7.7%), except malathion. Field mosquitoes had elevated levels of carboxylesterase (COE, 3.8-fold) and monooxygenase (P450, 2.1-fold) but not glutathione S-transferase (GST) compared to susceptible mosquitoes. When the field mosquitoes were pre-exposed to 4% PBO, the 50% lethal concentration of deltamethrin was reduced from 0.22% to 0.10%, compare to 0.02% for susceptible mosquitoes. Knockdown resistance gene mutation (L1014F) rate was 62% in field mosquitoes. PBO pre-exposure suppressed P450 enzyme expression levels by 25~34%, GST by 11%, and had no impact on COE enzyme expression. Even with the optimal PBO concentration and exposure duration, field mosquitoes had significantly higher P450 enzyme expression levels after PBO exposure compared to laboratory controls. These results demonstrate that PBO alone may not be enough to control highly pyrethroid resistant mosquitoes due to the multiple resistance mechanisms. Mosquito resistance to PBO-synergized insecticide should be closely monitored.

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“…gambiae and An. funestus was observed [37,38], emphasizing the importance of AChE as a crucial target for malaria vector control strategies.…”

Section: Metabolic Proteins As Insecticide Targetsmentioning

confidence: 99%

Anopheles metabolic proteins in malaria transmission, prevention and control: a review

et al. 2020

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The increasing resistance to currently available insecticides in the malaria vector, Anopheles mosquitoes, hampers their use as an effective vector control strategy for the prevention of malaria transmission. Therefore, there is need for new insecticides and/or alternative vector control strategies, the development of which relies on the identification of possible targets in Anopheles. Some known and promising targets for the prevention or control of malaria transmission exist among Anopheles metabolic proteins. This review aims to elucidate the current and potential contribution of Anopheles metabolic proteins to malaria transmission and control. Highlighted are the roles of metabolic proteins as insecticide targets, in blood digestion and immune response as well as their contribution to insecticide resistance and Plasmodium parasite development. Furthermore, strategies by which these metabolic proteins can be utilized for vector control are described. Inhibitors of Anopheles metabolic proteins that are designed based on target specificity can yield insecticides with no significant toxicity to non-target species. These metabolic modulators combined with each other or with synergists, sterilants, and transmission-blocking agents in a single product, can yield potent malaria intervention strategies. These combinations can provide multiple means of controlling the vector. Also, they can help to slow down the development of insecticide resistance. Moreover, some metabolic proteins can be modulated for mosquito population replacement or suppression strategies, which will significantly help to curb malaria transmission.

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Evidence of Insecticide Resistance to Pyrethroids and Bendiocarb in Anopheles funestus from Tsararano, Marovoay District, Madagascar

Cited by 8 publications

References 29 publications

Acetylcholinesterase (ace-1R) target site mutation G119S and resistance to carbamates in Anopheles gambiae (sensu lato) populations from Mali

Acetylcholinesterase (ace-1R) target site mutation G119S and resistance to carbamates in Anopheles gambiae (sensu lato) populations from Mali

Emerging mosquito resistance to piperonyl butoxide-synergized pyrethroid insecticide and its mechanism

Anopheles metabolic proteins in malaria transmission, prevention and control: a review

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