Evidence of<i>Borrelia lonestari</i>DNA in<i>Amblyomma americanum</i>(Acari: Ixodidae) Removed from Humans

Stromdahl, Ellen Y.; Williamson, Phillip; Kollars, Thomas M.; Evans, Sandra R.; Barry, Ryan K.; Vince, Mary A.; Dobbs, Nicole

doi:10.1128/jcm.41.12.5557-5562.2003

Cited by 115 publications

(68 citation statements)

References 34 publications

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“…34 The PCR were carried out in a total of 50 μL solution containing 1X PCR buffer minus Mg, 1.5 mM MgCl2, 0.2 mM dNTPs, 1 U of Platinum TaqDNA Polymerase, and 0.2 μM of each primer. For all PCR reactions, one positive control (DNA extracted from Borrelia anserina culture) and two negative controls (water) were included.…”

Section: Methodsmentioning

confidence: 99%

Survey for Tick-Borne Zoonoses in the State of Espirito Santo, Southeastern Brazil

Spolidorio¹,

Labruna²,

Machado³

et al. 2010

The American Journal of Tropical Medicine and Hygiene

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Section: Methodsmentioning

confidence: 99%

Survey for Tick-Borne Zoonoses in the State of Espirito Santo, Southeastern Brazil

Spolidorio¹,

Labruna²,

Machado³

et al. 2010

The American Journal of Tropical Medicine and Hygiene

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“…In Europe and Asia especially, the development of a uniform approach for the serological evaluation of LB is complicated by the prevalence of organisms from the three or more genospecies of B. burgdorferi sensu lato and by significant antigenic variation between and within each genospecies (Wang et al, 1999). Moreover, in the USA 'Borrelia lonestari' exposure may confound serological results (Stromdahl et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Antigenicity of borrelial protein BBK32 fragments in early Lyme borreliosis

Lahdenne

Sarvas

Kajanus

et al. 2006

Journal of Medical Microbiology

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Recombinantly produced borrelial BBK32 protein fragments originating from Borrelia burgdorferi sensu stricto, Borrelia garinii and Borrelia afzelii were evaluated as antigens in the serology of Lyme borreliosis (LB). In ELISA, a mid-portion hydrophilic fragment reacted with LB patient sera. Of the 23 patients with culture-or PCR-positive erythema migrans (EM), 43 % at diagnosis and 52 % at convalescence were positive for at least one Borrelia species-specific variant BBK32 fragment antigen. In parallel ELISAs with BBK32 whole proteins from the three borrelial subspecies as antigens, 17 % at diagnosis and 26 % at convalescence were positive. These results suggest that BBK32 protein fragments may improve the early IgG serology of LB compared to the BBK32 whole protein.

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“…20,23,25 Borrelia lonestari has additionally been identified in A. americanum ticks that were removed from humans, including patients from Kentucky. 26 Ehrlichia chaffeensis is a gram-negative obligate intracellular bacterium and the etiologic agent of human monocytotrophic ehrlichiosis. [27][28][29] Ehrlichia chaffeensis is maintained in a zoonotic cycle involving its principal reservoir, the white-tailed deer ( Odocoileus virginianus ) and A. americanum ticks.…”

Section: Introductionmentioning

confidence: 99%

Infection Prevalences of Common Tick-borne Pathogens in Adult Lone Star Ticks (Amblyomma americanum) and American Dog Ticks (Dermacentor variabilis) in Kentucky

Fritzen

Huang²,

Westby³

et al. 2011

The American Journal of Tropical Medicine and Hygiene

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Abstract. Rocky Mountain spotted fever, Lyme disease, and ehrlichiosis are tick-borne diseases that are reported annually in Kentucky. We conducted a survey to describe infection prevalence of tick-borne pathogens in Amblyomma americanum and Dermacentor variabilis ticks collected in Kentucky. During 2007-2008, we collected 287 ticks (179 D. variabilis and 108 A. americanum ) from canine, feral hog, horse, raccoon, white-tailed deer, and human hosts in six counties in Kentucky. Ticks were screened for Rickettsia spp., Borrelia spp., and Ehrlichia spp. by using polymerase chain reaction. Forty-one (14.3%) ticks (31 A. americanum and 10 D. variabilis ) were polymerase chain reaction-positive for a Rickettsia spp. Fourteen (4.9%) ticks (6 A. americanum and 8 D. variabilis ) were positive for E. chaffeensis , and 4 A. americanum (1.4%) were positive for E. ewingii . One (0.4%) A. americanum was positive for Borrelia lonestari .

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Evidence ofBorrelia lonestariDNA inAmblyomma americanum(Acari: Ixodidae) Removed from Humans

Abstract: We used a nested PCR with

Cited by 115 publications

References 34 publications

Survey for Tick-Borne Zoonoses in the State of Espirito Santo, Southeastern Brazil

Survey for Tick-Borne Zoonoses in the State of Espirito Santo, Southeastern Brazil

Antigenicity of borrelial protein BBK32 fragments in early Lyme borreliosis

Infection Prevalences of Common Tick-borne Pathogens in Adult Lone Star Ticks (Amblyomma americanum) and American Dog Ticks (Dermacentor variabilis) in Kentucky

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