Evidence of genetic recombination in wheat yellow rust populations of a Chinese oversummering area

Mboup, Mamadou; Leconte, Marc; Gautier, Angélique; Wan, Anmin; Chen, W.; Vallavieille‐Pope, C. de; Enjalbert, Jérôme

doi:10.1016/j.fgb.2008.12.007

Cited by 103 publications

(100 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Restricted clonal populations in these regions were found Hovmøller and Justesen 2007;Enjalbert et al 2005;Steele et al 2001). A recombination signature in P. striiformis has been suggested in Gansu Province (Mboup et al 2009;Duan et al 2010). Amplified fragment length polymorphism (AFLP) markers for population studies on P. striiformis were generated and the set of these markers was intensively used to study the population genetics of this pathogen in Europe and North America (Markell and Milus 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Since Gansu Province shows high genetic diversity of the P. striifornis populations (Mboup et al 2009;Duan et al 2010) and is the major area of China where the pathogen can oversummer to complete its life cycle, that affects the whole regional epidemics in China (Zeng and Luo 2006), we also collected isolates from this province as a reference for comparison in population structure between Yunnan and Gansu Provinces. In this study, we used AFLP analysis to test the degrees of genetic differentiation among populations at different geographic regions in Yunnan Province.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Analysis of population structure of Puccinia striiformis in Yunnan Province of China by using AFLP

et al. 2010

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analysis of population structure of Puccinia striiformis in Yunnan Province of China by using AFLP

et al. 2010

View full text Add to dashboard Cite

show abstract

“…According to previous studies, population genetics analysis with molecular markers such as Microsatellite (SSR), Restriction Fragment Length Polymorphism (RFLP), AFLP, etc., required about 50-100 ng DNA template and up to micrograms should be needed in high-throughput DNA sequencing (Lu et al 2009;Mboup et al 2009;Lasken 2007). In this study, the quantity of pathogenic genomic DNA within the 50 μL amplified DNA products were above 10 μg from fresh urediospores, and above 2.5 μg from stored urediospores, thus the quantity of DNA obtained via MDA method from 20 to 30 urediospores could satisfy all these subsequent analyses.…”

Section: Discussionmentioning

confidence: 60%

Multiple displacement amplification of whole genomic DNA from urediospores of Puccinia striiformis f. sp. tritici

Zhang

2015

Curr Genet

View full text Add to dashboard Cite

Biotrophic fungi, such as Puccinia striiformis f. sp. tritici, because they cannot be cultured on nutrient media, to obtain adequate quantity of DNA for molecular genetic analysis, are usually propagated on living hosts, wheat plants in case of P. striiformis f. sp. tritici. The propagation process is time-, space- and labor-consuming and has been a bottleneck to molecular genetic analysis of this pathogen. In this study we evaluated multiple displacement amplification (MDA) of pathogen genomic DNA from urediospores as an alternative approach to traditional propagation of urediospores followed by DNA extraction. The quantities of pathogen genomic DNA in the products were further determined via real-time PCR with a pair of primers specific for the β-tubulin gene of P. striiformis f. sp. tritici. The amplified fragment length polymorphism (AFLP) fingerprints were also compared between the DNA products. The results demonstrated that adequate genomic DNA at fragment size larger than 23 Kb could be amplified from 20 to 30 urediospores via MDA method. The real-time PCR results suggested that although fresh urediospores collected from diseased leaves were the best, spores picked from diseased leaves stored for a prolonged period could also be used for amplification. AFLP fingerprints exhibited no significant differences between amplified DNA and DNA extracted with CTAB method, suggesting amplified DNA can represent the pathogen's genomic DNA very well. Therefore, MDA could be used to obtain genomic DNA from small precious samples (dozens of spores) for molecular genetic analysis of wheat stripe rust pathogen, and other fungi that are difficult to propagate.

show abstract

“…The urediniospores and the hyphal stage on wheat are dikaryotic, but at the genetic level Pst behaves as a functional diploid. Pst populations have a high level of diversity at both the avirulence/ virulence (phenotypic) and molecular (genotypic) levels (Mboup et al, 2009;Duan et al, 2010). Genetic variation is created by mutation and recombination, and selection determines the phenotypic frequencies that characterize the populations.…”

Section: Introductionmentioning

confidence: 99%

“…Asexual variation in cereal fungi (sometimes called "somatic recombination") has been documented (Little and Manners, 1967) in laboratory studies and occasionally shown to be a significant cause of variation in the field (Park and Wellings, 2012). The recent prediction from population studies of the likelihood of sexual recombination in Pst in China (Mboup et al, 2009), and subsequent demonstration of Berberis spp. as an alternative host, firstly for P. striiformis f. sp.…”

Section: Introductionmentioning

confidence: 99%

Screening for simple sequence repeat markers in Puccinia striiformis tritici based on genomic sequence

Zhan

Wang

Luo

et al. 2015

J. Zhejiang Univ. Sci. B

View full text Add to dashboard Cite

Puccinia striiformis f. sp. tritici (Pst) is the obligate biotrophic fungus responsible for stripe rust wheat. In this study, we developed and characterized 20 polymorphic microsatellite markers from the genomic sequence of an isolate of Chinese Pst race CY32. Polymorphism at each simple sequence repeat (SSR) locus was determined using 32 Pst isolates from 7 countries. The number of alleles varied from 2 to 7 across isolates, and the observed and expected heterozygosities ranged from 0.33 to 0.97 (mean 0.62) and 0.23 to 0.73 (mean 0.51), respectively. As expected the genomic SSR markers were more polymorphic than the expressed sequence tag (EST)-SSR markers developed previously. These markers will be more useful for population genetics and molecular genetics studies in Pst.

show abstract

Evidence of genetic recombination in wheat yellow rust populations of a Chinese oversummering area

Cited by 103 publications

References 51 publications

Analysis of population structure of Puccinia striiformis in Yunnan Province of China by using AFLP

Analysis of population structure of Puccinia striiformis in Yunnan Province of China by using AFLP

Multiple displacement amplification of whole genomic DNA from urediospores of Puccinia striiformis f. sp. tritici

Screening for simple sequence repeat markers in Puccinia striiformis tritici based on genomic sequence

Contact Info

Product

Resources

About