Evidence of chronic inflammation in retina excised after relaxing retinotomy for anterior proliferative vitreoretinopathy

Limb, G. Astrid; Chignell, A H; Woon, H; Green, W; Cole, C J; Dumonde, D. C.

doi:10.1007/bf00430412

Cited by 12 publications

(12 citation statements)

References 28 publications

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“…The role of infl ammatory processes in retinas with PVR was suggested by Limb et al [31] , but their fi ndings were based only on anterior and advanced cases of PVR. In our study, the retinal tissue was obtained from the middle periphery, close to the equator.…”

Section: Discussionmentioning

confidence: 99%

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Intraretinal Immunohistochemistry Findings in Proliferative Vitreoretinopathy with Retinal Shortening

Pastor¹,

Mendez

Fuente³

et al. 2006

Ophthalmic Res

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To report the major intraretinal pathological changes in retinas with proliferative vitreoretinopathy (PVR) and retinal shortening, 13 human retinal samples from postoperative PVR after primary surgery for retinal detachment were immunostained for vimentin, glial fibrillary acidic protein (GFAP), cytokeratins, and CD68. One more sample was studied with electron microscopy. Retinal disorganization, neuronal loss, and gliosis were observed in 12 out of 13 samples, but all 13 were positive for GFAP. Müller cell processes showed different degrees of intermediate filament hyperplasia. CD68-positive cells were present in 11 of 13 retinal samples. Conclusion: A gliotic response plays a major role in retinal shortening in PVR. In addition, the presence of macrophage-like cells in retinal tissues suggests a possible role of these cells in the pathogenesis of this variety of PVR.

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Section: Discussionmentioning

confidence: 99%

“…Limb et al [31] suggested that the retinal infl ammatory process culminated in the formation of retinal membranes. Our results suggest that retinal gliosis may also be part of the pathologic changes in PVR, at least in a signifi cant percentage of cases.…”

Section: Discussionmentioning

confidence: 99%

Intraretinal Immunohistochemistry Findings in Proliferative Vitreoretinopathy with Retinal Shortening

Pastor¹,

Mendez

Fuente³

et al. 2006

Ophthalmic Res

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“…Both, VEGF and TNF-α are not present in the retinal tissue of healthy subjects [11,12]. In human RPE cells however, VEGF expression was demonstrated.…”

Section: Discussionmentioning

confidence: 99%

Detection of Vascular Endothelial Growth Factor and Tumor Necrosis Factor Alpha in Epiretinal Membranes of Proliferative Diabetic Retinopathy, Proliferative Vitreoretinopathy and Macular Pucker

et al. 1998

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Purpose: To evaluate epiretinal membranes in proliferative eye disease for the presence of vascular endothelial growth factor (VEGF) and tumor necrosis factor α (TNF-α). Methods: Membranes were surgically removed from 66 patients with proliferative diabetic retinopathy (PDR), proliferative vitreoretinopathy (PVR) and macular pucker (MP). Cytokine concentrations were determined by ELISA (VEGF) and bioassay (TNF-α). Results: VEGF was detected in all 66 membranes investigated. The highest VEGF values were found in patients with type I diabetes (mean = 5,994 pg/mg protein). In patients with type II diabetes, the values were at a mean of 1,242 pg/mg protein. When coagulation therapy was performed for longer than 3 months prior to surgery, VEGF was significantly (p < 0.05) reduced. Intermediate levels of VEGF were found in PVR membranes (mean = 1,417 pg/mg protein). The lowest activity was found in MP (mean = 216 pg/mg protein). In contrast, TNF-α was present in 16 PDR membranes, 9 PVR membranes and 8 MP membranes. Conclusion: The presence of VEGF in all membranes investigated indicates that this cytokine plays an important role in angiogenesis in ischemic retinal disease and in membrane growth in proliferative disorders.

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“…Alkaline phosphatase-anti-alkaline phosphatase (APAAP) staining was performed according to previously published methods (16) as follows: Fixed slides were washed for 10 min in Trisbuffered saline pH 7.4 (0.05 M Tris in 0.15 M NaCl:TBS) and incubated for 20 min at room temperature (RT) with TBS containing 0.5% blocking reagent (Boehringer Mannheim, Germany). Cells were then incubated for 30 min at RT with 50 l/ well optimal dilutions of one of seven mouse IgG monoclonal antibodies (mAb): Bra-11, detecting CD45 (170-220 kD); KD3, detecting CD45RA (220 and 205 kDa glycoproteins); UCHL1, detecting CD45RO (180 kDa glycoprotein); PCK-26, detecting cytokeratin type 8; CY-90, detecting cytokeratin peptide 18; (Sigma Chemical Co, England); M814 detecting CD68 (Dako, UK) and RRI, detecting ICAM-1 (Boehringer Ingelheim, USA).…”

Section: Immunocytochemical (Alkaline Phosphatase-anti-alkaline Phospmentioning

confidence: 99%

Expression of hematopoietic cell markers by retinal pigment epithelial cells

Limb

Earley

et al. 1997

Current Eye Research

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The observations suggest that hematopoietic cell markers are constitutively expressed on RPE cells and that functions governed by these molecules are not influenced by pro-inflammatory signals. Expression of hematopoietic molecules by RPE cells may influence the macrophage-like properties of these cells and may also aid in the identification of RPE cells during pathological processes, particularly in the proliferative retinopathies, where these cells undergo phenotypic and functional changes.

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Evidence of chronic inflammation in retina excised after relaxing retinotomy for anterior proliferative vitreoretinopathy

Abstract: The present findings indicate that retina from eyes with advanced PVR may itself be subject to inflammatory changes, and indicate that the PVR process is not limited to retinal membranes, but involves a more widespread distribution of inflammation than is generally appreciated.

Cited by 12 publications

References 28 publications

Intraretinal Immunohistochemistry Findings in Proliferative Vitreoretinopathy with Retinal Shortening

Intraretinal Immunohistochemistry Findings in Proliferative Vitreoretinopathy with Retinal Shortening

Detection of Vascular Endothelial Growth Factor and Tumor Necrosis Factor Alpha in Epiretinal Membranes of Proliferative Diabetic Retinopathy, Proliferative Vitreoretinopathy and Macular Pucker

Expression of hematopoietic cell markers by retinal pigment epithelial cells

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