“…Previously, many sero-epidemiological investigations of viruses of public health importance, i.e., DENV, CCHFV, Rift Valley fever virus, WNV, Usutu virus, CHIKV, Bourbon virus, Hantavirus, ZIKV, and SARS-CoV-2, have been carried out to confirm the presence of virus-specific IgM and IgG antibodies, and subsequently, case confirmation using gold standard PRNTs or by virus isolation [74][75][76][77][78][79][80][81][82][83][84]. Due to cross-reactivity amongst the arboviruses, cell culture-based NTs are the preferred serological tool for case confirmation.…”