Evidence of a new metabolic pathway of 5-fluorouracil in Escherichia coli from in vivo 19F-NMR spectroscopy

19F nuclear magnetic resonance study of fluoropyrimidine metabolism in strains of Candida glabrata with specific defects in pyrimidine metabolism

“…In the filamentous Nectria fungus, the catabolic pathway is operative under normal conditions of culture (20). The metabolism of 5-FC in this fungus is similar to that occurring in mice (23) and bacteria (14).…”

“…In addition, it has been used to demonstrate tumor trapping of 5-FU in tumorbearing humans and rabbits (32). NMR spectroscopy has also been successful in demonstrating the existence of two distinct pathways of 5-FU degradation in E. coli (14).'9F NMR spectroscopy has also been employed in a preliminary study of the uptake and metabolism of the clinical antifungal drug 5-flucytosine (5-FC) in the medically important yeast Candida albicans (10). A similar but more quantitative approach was adopted to study 5-FC metabolism in three C. albicans strains and one Candida tropicalis * Corresponding author.…”

“…In addition, it has been used to demonstrate tumor trapping of 5-FU in tumorbearing humans and rabbits (32). NMR spectroscopy has also been successful in demonstrating the existence of two distinct pathways of 5-FU degradation in E. coli (14).…”