Evidence from Gene Mutations in Neurospora for Close Metabolic Relationships Among Ornithine, Proline, and α-Amino-δ-Hydroxy Valeric Acid

Srb, Adrian M.; Fincham, J. R. S.; Bonner, David M.

doi:10.2307/2438029

Cited by 24 publications

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“…By study of the ability of various naturally occurring compounds to replace the arginine requirement of fungus mutants it has been possible to reveal metabolic relationships and to locate genetical blocks in the reaction chains involved. In Neurospora mutant strains i3-8± 5-7 116-4+ 13-6 108-0+ 8-2 75-8+ 9-7 4-1 ± 1-2 have been obtained which can satisfy their arginine requirement with proline (Srb, 1946) and Srb, Fincham & Bonner (1950) have shown that certain arginineless strains can utilize for growth either arginine, ornithine, citruUine, proline or a-amino-S-hydroxyvaleric acid. Bonner (1946) has shown that with arginineless mutants oi Penicillium the requirement can be met by arginine, ornithine, citrulline or proline, and that one strain of P. chrysogenum (24033) also responded to glutamic acid.…”

Section: ) Preparation and Growth-promoting Properties Of A Synthetimentioning

confidence: 99%

Studies on the Growth of Excised Roots

Skinner

Street

1954

New Phytologist

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Section: ) Preparation and Growth-promoting Properties Of A Synthetimentioning

confidence: 99%

Studies on the Growth of Excised Roots

Skinner

Street

1954

New Phytologist

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“…The physiology and genetics of arginaseless (aga) mutants of Neurospora is reported here, together with evidence for a role of arginase in polyamine metabolism under certain conditions. Our rationale for mutant selection was based on the fact that arginine catabolism can provide GSA in proline mutants (e.g., arg-8) blocked in the synthesis of GSA (17). In the double mutant arg-S, arg-8, the endogenous synthesis of ornithine and GSA are both blocked.…”

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Arginaseless Neurospora : Genetics, Physiology, and Polyamine Synthesis

1970

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Four arginaseless mutants of Neurospora crassa have been isolated. All carry mutations which lie at a single locus, aga, on linkage group VIIR. A study of aga strains shows the arginase reaction to be the major, perhaps the only, route of arginine consumption in Neurospora other than protein synthesis. Ornithine-5transaminase, the second enzyme of the arginine catabolic pathway, is present and normally inducible by arginine in aga strains, and ornithine transcarbamylase, an enzyme of arginine synthesis, also has normal activity. Arginine inhibits the growth of aga strains. The inhibition can be reversed by spermidine, putrescine (1,4-diaminobutane), or ornithine. The results suggest that ornithine is the major source of the putrescine moiety of polyamines in Neurospora, and that putrescine is an essential growth factor for this organism. The inhibition of aga strains by arginine can be attributed to feedback inhibition of ornithine synthesis by arginine, combined with the complete lack of ornithine normally provided by the arginase reaction.

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“…Prolinerequiring mutants which have been found in A. niduln7zs, V. i7iaeqz~nlis, N . crassa (Srb et al, 1950), P. notnturn-cb~ysoge1iz~7~~ and U . bordei have not been found in Co7ziochaetn.…”

Section: Discussionmentioning

confidence: 99%

MUTATION SPECTRUM IN THE FUNGUS CONIOCHAETA VELUTINA

Paterson¹,

Tan²,

Cooke³

1969

Can. J. Genet. Cytol.

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Auxotrophic, n~orphological, sensitive and suppressor mutant strains of Co7?iocbnetn vel~rti~zn are dcscribed. Of 1 3 3 1 auxotrophic mutants isolated, all but 58 have been characterized as to their specific rcquiren~cnn. T h e mutation spectrum obtained on malt medium differs in several respects from that on complete medium. Arginine, adenine, cysteinc, rnethionine, histidine and pyrimidine requiring mutants were most frequently isolated. Of 1 2 diauxotrophs tested gc~letically, 7 proved to be double mutants, but the remaining 5 behaved genetically as single mutann rvith a double requirement. Four of these required both adenine and histidine for growth and [he other both pl~enylalanine and ryrosine. Cystcine-sensitive murants and hisridinc-sensitive strains are dcscribed. Other mutann isolated included a variety of morphological, conidial and colour variants, UV-sensitive srrains, canavaninc-sensitive srrains and arginine-suppressor murations.

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Evidence from Gene Mutations in Neurospora for Close Metabolic Relationships Among Ornithine, Proline, and α-Amino-δ-Hydroxy Valeric Acid

Cited by 24 publications

References 0 publications

Studies on the Growth of Excised Roots

Studies on the Growth of Excised Roots

Arginaseless Neurospora : Genetics, Physiology, and Polyamine Synthesis

MUTATION SPECTRUM IN THE FUNGUS CONIOCHAETA VELUTINA

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