1976
DOI: 10.1016/0022-4731(76)90168-0
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Evidence for the presence of 6-keto-oestradiol-17β in human plasma—Implications for oestradiol-17β radioimmunoassays

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Cited by 11 publications
(3 citation statements)
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“…Hormonal assessment Oestrone-3-glucuronide was assayed in urine by the immunoassay method of Baker et al (1979) using an antiserum with a cross-reactivity of 2.7% with oestrone, 10.2% with oestradiol-l7/3, 6.1 % with oestradiol-1701 and 2-6 % with epi-oestriol. This represented minimal cross-reactivity given the very small amount of oestrone and oestradiol-17/3 in human urine (Bolton & Rutherford, 1976). The sensitivity of the assay was 9 pg per assay tube equivalent to 3.8 nmol/l, with intra-and interassay coefficients of variation of 13.1 and 12.9 %.…”
Section: E T H O D Smentioning
confidence: 94%
“…Hormonal assessment Oestrone-3-glucuronide was assayed in urine by the immunoassay method of Baker et al (1979) using an antiserum with a cross-reactivity of 2.7% with oestrone, 10.2% with oestradiol-l7/3, 6.1 % with oestradiol-1701 and 2-6 % with epi-oestriol. This represented minimal cross-reactivity given the very small amount of oestrone and oestradiol-17/3 in human urine (Bolton & Rutherford, 1976). The sensitivity of the assay was 9 pg per assay tube equivalent to 3.8 nmol/l, with intra-and interassay coefficients of variation of 13.1 and 12.9 %.…”
Section: E T H O D Smentioning
confidence: 94%
“…In the radioimmunoassay of free steroids the use of an artificial bridge, e.g. hemisuccinate or carboxymethyl oxime, has given rise to strong cross-reactions with similar steroids differing at the position of conjugation (Bolton & Rutherford, 1976). Finally, the availability of 3H-labelled steroid glucuronides makes it possible to use a labelled ligand that closely resembles the complete hapten conjugate.…”
mentioning
confidence: 99%
“…Oestradiol-1 7B(E2) Serum E2 levels were measured after extraction into diethyl ether and back extraction into sodium hydroxide solution followed by neutralization. The anti-serum used was of high specificity and was raised to 6-(o-carboxmethyl)oxime bovine serum albumin (BSA) conjugate as previously described (Bolton & Rutherford, 1976). Solvent and water blanks gave values which were indistinguishable from those found in 'zero' tubes of the standard curve.…”
Section: Assay Methodsmentioning
confidence: 99%