Evidence for the presence of 6-keto-oestradiol-17β in human plasma—Implications for oestradiol-17β radioimmunoassays

Bolton, A.E.; Rutherford, F.

doi:10.1016/0022-4731(76)90168-0

Cited by 11 publications

(3 citation statements)

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“…Hormonal assessment Oestrone-3-glucuronide was assayed in urine by the immunoassay method of Baker et al (1979) using an antiserum with a cross-reactivity of 2.7% with oestrone, 10.2% with oestradiol-l7/3, 6.1 % with oestradiol-1701 and 2-6 % with epi-oestriol. This represented minimal cross-reactivity given the very small amount of oestrone and oestradiol-17/3 in human urine (Bolton & Rutherford, 1976). The sensitivity of the assay was 9 pg per assay tube equivalent to 3.8 nmol/l, with intra-and interassay coefficients of variation of 13.1 and 12.9 %.…”

Section: E T H O D Smentioning

confidence: 94%

Changes in energy expenditure during the menstrual cycle

James²,

1989

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1. Eight women were studied under metabolic-ward conditions while consuming a constant diet throughout a single menstrual cycle. Basal body temperature, salivary and urinary hormone concentrations were used in monitoring the cycle and designing the study so that whole-body calorimetry for 36 h was conducted at four phases of the cycle in relation to the time of ovulation.2. The metabolic rate during sleep showed cyclical changes, being lowest in the late follicular phase and highest in the late luteal phase. The increase amounted to 6 1 (SD 27) YO. Energy expenditure (24 h) also increased but the change was not statistically significant (P > 0.05). Exercise efficiency did not change during the cycle.3. There were no significant changes in plasma thyroxine, 3,5,3'-triiodothyronine or free 3,5,3'-triiodothyronine concentrations to explain the metabolic rate changes; nor did they relate to urinary luteinizing hormone, pregnanediol-3a-glucuronide or oestrone-3-glucuronide excretion rates. No link with salivary cortisol or progesterone concentrations was observed, but there was a small inverse relation between the individual increase in sleeping metabolic rate and the subjects' falling ratio of urinary oestrone-3-glucuronide : pregnanediol-3a-glucuronide.The variability in energy balance of women during the menstrual cycle has been of interest since research workers in the 1920s examined the problem. At first there was little appreciation that changes might occur at times other than menstruation itself and whether systematic changes did occur was disputed (Snell et al. Hitchcock & Wardwell, 1929). Some authors failed to find any effect, whereas others showed an increase in basal metabolic rate (BMR) during the premenstrual phase of the menstrual cycle. In all these early studies the dietary intake was uncontrolled so that any changes in food intake could have affected BMR. More recently, Solomon et al. (1982) overcame this problem by measuring BMR in the course of studies which involved changing the protein intake in sequential menstrual cycles. Thus, the diet was maintained constant during a single cycle but the protein intake varied from cycle to cycle. The BMR was found to increase significantly during the luteal phase of the cycle. M E T H O D SThe purpose of the present study was to maintain food intake constant throughout the cycles of eight women who remained under metabolic-ward conditions. Total faecal and urine collections were made to quantify energy and protein losses, and thyroidal, cortisol and sex hormones were also monitored throughout the cycles to assess whether they might influence energy expenditure. Energy expenditure itself was measured under a variety of * For reprintshttps://www.cambridge.org/core/terms. https://doi

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Section: E T H O D Smentioning

confidence: 94%

Changes in energy expenditure during the menstrual cycle

James²,

1989

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“…In the radioimmunoassay of free steroids the use of an artificial bridge, e.g. hemisuccinate or carboxymethyl oxime, has given rise to strong cross-reactions with similar steroids differing at the position of conjugation (Bolton & Rutherford, 1976). Finally, the availability of 3H-labelled steroid glucuronides makes it possible to use a labelled ligand that closely resembles the complete hapten conjugate.…”

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confidence: 99%

The direct radioimmunoassay of oestrogen glucuronides in human female urine

Baker¹,

Jennison²,

Kellie³

1979

Biochemical Journal

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Radioimmunoassays for five oestrogen metabolites in urine are described; they are oestrone 3-glucuronide, oestradiol 3-glucuronide, oestradiol 17 beta-glucuronide, oestriol 3-glucuronide and oestriol 16 alpha-glucuronide. These assays have proved accurate and reliable and can be performed rapidly; they have been carried out directly in diluted menstrual cycle urine and pregnancy urine. No sample pretreatment was required. Preliminary results suggest that clinically useful information can be obtained by performing these assays on random urine specimens.

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“…Oestradiol-1 7B(E2) Serum E2 levels were measured after extraction into diethyl ether and back extraction into sodium hydroxide solution followed by neutralization. The anti-serum used was of high specificity and was raised to 6-(o-carboxmethyl)oxime bovine serum albumin (BSA) conjugate as previously described (Bolton & Rutherford, 1976). Solvent and water blanks gave values which were indistinguishable from those found in 'zero' tubes of the standard curve.…”

Section: Assay Methodsmentioning

confidence: 99%

Gonadal‐pituitary Hormone Levels in Gynaecomastia

I¹,

Bolton²,

Cameron³

et al. 1980

Clinical Endocrinology

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Plasma levels of LH, FSH, oestradiol 17 beta and testosterone were measured in twenty-two men with gynaecomastia. The group was divided by age into those under and those over 50-years-old and the mean hormone levels of the two groups were compared with two groups of age-matched normal men. In the young men with gynaecomastia LH and consequently the LH:FSH ratio was lower than in controls. Older patients with gynaecomastia had higher values of both LH and FSH than normal controls but the LH:FSH ratio was similar in the two groups. A pulsatile pattern of LH was present only in the young controls. Older controls had higher FSH levels than younger controls. In older men with gynaecomastia oestradiol levels and the oestradiol:testosterone ratio were higher than in those without.

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Evidence for the presence of 6-keto-oestradiol-17β in human plasma—Implications for oestradiol-17β radioimmunoassays

Cited by 11 publications

References 1 publication

Changes in energy expenditure during the menstrual cycle

Changes in energy expenditure during the menstrual cycle

The direct radioimmunoassay of oestrogen glucuronides in human female urine

Gonadal‐pituitary Hormone Levels in Gynaecomastia

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