Evidence for the functional involvement of members of the TRP channel family in the uptake of Na+ and NH4 + by the ruminal epithelium

Rosendahl, Julia; Braun, Hannah-Sophie; Schrapers, Katharina T.; Martens, H.; Stumpff, Friederike

doi:10.1007/s00424-016-1835-4

Cited by 33 publications

(110 citation statements)

References 67 publications

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“…Modified from Wilkens et al (2016). menthol enhanced Ca net flux rates determined for ovine rumen epithelia in Ussing chambers from 8.60 ± 1.43 to 13.24 ± 0.91 nmol/cm 2 •h and Na-mediated short-circuit currents (Rosendahl et al, 2016). In dairy cows, oral administration of 1.2 g essential oils with menthol as the major compound increased plasma Ca from 2.46 to 2.53 mM and decreased plasma urea from 4.28 to 3.92 mM (Braun et al, 2019).…”

Section: Transcellular Pre-intestinal Calcium Absorptionmentioning

confidence: 99%

Review: Regulation of gastrointestinal and renal transport of calcium and phosphorus in ruminants

Wilkens¹,

Muscher-Banse²

2020

Animal

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In comparison to monogastric animals, ruminants show some peculiarities in respect to the regulation of mineral homeostasis, which can be regarded as a concerted interplay between gastrointestinal absorption, renal excretion and bone mobilisation to maintain physiological Ca and phosphate (Pi) concentrations in serum. Intestinal absorption of Ca or Pi is mediated by two general mechanisms: paracellular, passive transport dominates when luminal Ca or Pi concentrations are high and transcellular. The contribution of active transport becomes more important when dietary Ca or Pi supply is restricted or the demand increased. Both pathways are modulated directly by dietary interventions, influenced by age and regulated by endocrine factors such as 1,25-dihydroxyvitamin D3. Similar transport processes are observed in the kidney. After filtration, Ca and Pi are resorbed along the nephron. However, as urinary Ca and Pi excretion is very low in ruminants, the regulation of these renal pathways differs from that described for monogastric species, too. Furthermore, salivary secretion, as part of endogenous Pi recycling, and bone mobilisation participate in the maintenance of Ca and Pi homeostasis in ruminants. Saliva contains large amounts of Pi for buffering rumen pH and to ensure optimal conditions for the rumen microbiome. The skeleton is a major reservoir of Ca and Pi to compensate for discrepancies between demand and uptake. But alterations of the regulation of mineral homeostasis induced by other dietary factors such as a low protein diet were observed in growing ruminants. In addition, metabolic changes, for example, at the onset of lactation have pronounced effects on gastrointestinal mineral transport processes in some ruminant species. As disturbances of mineral homeostasis do not only increase the risk of the animals to develop other diseases, but are also associated with protein and energy metabolism, further research is needed to improve our knowledge of its complex regulation.

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Section: Transcellular Pre-intestinal Calcium Absorptionmentioning

confidence: 99%

Review: Regulation of gastrointestinal and renal transport of calcium and phosphorus in ruminants

Wilkens¹,

Muscher-Banse²

2020

Animal

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“…Our own interest in TRP channels was sparked when attempting to identify proteins involved in ammonia transport by gastrointestinal epithelia [ 11 – 13 ]. Large quantities of ammonia are produced in the fermentative parts of the gut from microbial degradation of nitrogenous compounds and subsequently absorbed.…”

Section: Introductionmentioning

confidence: 99%

The bovine TRPV3 as a pathway for the uptake of Na+, Ca2+, and NH4+

et al. 2018

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Absorption of ammonia from the gastrointestinal tract results in problems that range from hepatic encephalopathy in humans to poor nitrogen efficiency of cattle with consequences for the global climate. Previous studies on epithelia and cells from the native ruminal epithelium suggest functional involvement of the bovine homologue of TRPV3 (bTRPV3) in ruminal NH4+ transport. Since the conductance of TRP channels to NH4+ has never been studied, bTRPV3 was overexpressed in HEK-293 cells and investigated using the patch-clamp technique and intracellular calcium imaging. Control cells contained the empty construct. Divalent cations blocked the conductance for monovalent cations in both cell types, with effects higher in cells expressing bTRPV3. In bTRPV3 cells, but not in controls, menthol, thymol, carvacrol, or 2-APB stimulated whole cell currents mediated by Na+, Cs+, NH4+, and K+, with a rise in intracellular Ca2+ observed in response to menthol. While only 25% of control patches showed single-channel events (with a conductance of 40.8 ± 11.9 pS for NH4+ and 25.0 ± 5.8 pS for Na+), 90% of bTRPV3 patches showed much larger conductances of 127.8 ± 4.2 pS for Na+, 240.1 ± 3.6 pS for NH4+, 34.0 ± 1.7 pS for Ca2+, and ~ 36 pS for NMDG+. Open probability, but not conductance, rose with time after patch excision. In conjunction with previous research, we suggest that bTRPV3 channels may play a role in the transport of Na+, K+, Ca2+ and NH4+ across the rumen with possible repercussions for understanding the function of TRPV3 in other epithelia.

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“…Importantly, Mg is involved in the gating of numerous ion channels (Vemana, Pandey, & Larsson, ). In the rumen, non‐selective cation channels such as TRPV3 (transient receptor potential vanilloid type 3 channel) are regulated by Mg in a voltage‐dependent manner, with possible implications for ruminal ion transport (Leonhard‐Marek, Stumpff, Brinkmann, Breves, & Martens, ; Rosendahl, Braun, Schrapers, Martens, & Stumpff, ; Schrapers, Sponder, Liebe, Liebe, & Stumpff, ; Stumpff & Martens, ).…”

Section: Introductionmentioning

confidence: 99%

Assessment of magnesium intake according to requirement in dairy cows

Martens

Stumpff

2019

Animal Physiology Nutrition

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To date, no specific hormonal regulation system has been identified for homoeostatic control of the essential mineral Mg. In cattle, the maintenance of physiological plasma Mg concentration depends on gastrointestinal absorption, primarily from the rumen, which serves as a pool for covering the requirement. Whereas a possible surplus (absorption greater than requirement) is rapidly excreted by the kidneys, a shortage (absorption lower than requirement) cannot be compensated for by mobilization from the large Mg pool in bones or soft tissue, so that the maintenance of the necessary physiological Mg concentration in plasma relies on continuous and sufficient absorption. Our knowledge concerning the site and mechanisms of Mg absorption has improved during the last few decades, and meta‐analyses of the absorption of Mg in dairy cows have shown that the K content has a pronounced negative effect on Mg digestibility. The current recommendations of Mg intake propose a constant percentage of Mg and emphasize the depressive effect of high potassium (K) intake on Mg absorption. The current knowledge about the antagonism between K intake and Mg absorption allows a more flexible solution which includes the K content of the diet. An assessment of Mg intake is proposed that incorporates the improved knowledge of Mg absorption, metabolism and requirement. Within this framework, an equation is derived that allows a prediction of the amount of Mg required to compensate for dietary K content, the goal being to avoid both possible undernutrition or an unnecessary surplus of dietary Mg.

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Evidence for the functional involvement of members of the TRP channel family in the uptake of Na+ and NH4 + by the ruminal epithelium

Cited by 33 publications

References 67 publications

Review: Regulation of gastrointestinal and renal transport of calcium and phosphorus in ruminants

Review: Regulation of gastrointestinal and renal transport of calcium and phosphorus in ruminants

The bovine TRPV3 as a pathway for the uptake of Na+, Ca2+, and NH4+

Assessment of magnesium intake according to requirement in dairy cows

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