Evidence for the association of mastrevirus, cucumovirus and phytoplasma with chickpea stunt disease and their putative insect vectors in India

Shreenath, Y. S.; Saritha, R. K.; Basavaraj, Y. B.; Pant, R. P.; Sagar, D.; Arya, Meenakshi; Rao, G. P.

doi:10.1007/s10658-020-02003-y

Cited by 7 publications

(12 citation statements)

References 37 publications

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“…Four different group of viruses (mastrevirus, luteovirus, polerovirus and cucumovirus) have been reported associated with CpS disease worldwide (Kanakala and Kuria 2019 ). Chickpea phyllody caused by phytoplasma is another emerging problem of chickpea in some major chickpea growing countries (Shreenath et al 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…cDNA synthesis was performed by using a Verso cDNA synthesis Kit (Thermo scientific). The cDNA was used for PCR amplifications of BLRV using the primer pair BLRV-3/ BLRV-5 (Ortiz et al 2005 ), cucumber mosaic virus (CMV) using the primer pair CPF/CPR (Shreenath et al 2020 ) and Lu1 + Eco side/Lu4 + Eco primer pair for the plant viruses belonging to the family Luteoviridae (Robertson et al 1991 ).…”

Section: Methodsmentioning

confidence: 99%

“…The major symptoms reported by different workers associated with stunt disease include leaf reddening and stunting in desi-type and leaf yellowing in kabuli-type along with browning of vascular tissues in collar regions. In early stage of the crop, diseased plants show more severe stunting symptoms (Nene and Reddy 1987 ; Kanakala and Kuria 2019 ; Shreenath et al 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…CpCDV was later reported to affect chickpea and other legume crops in Australia, Egypt, Iraq, Iran, Oman, Pakistan, Sudan, Syria and Yemen (Kanakala and Kuria 2019 ). Although CpCDV has been reported as a major virus associated with CpSD, the presence of luteovirus and cucumovirus is also documented from India (Kanakala et al 2013 ; Shreenath et al 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…The phytoplasma strain associated with chickpea phyllody was reported to be transmitted by O. albicinctus in Pakistan (Akhtar et al 2009 ). The incidence of phytoplasma associated with chickpea has been reported increasing throughout major chickpea growing areas of India (Pallavi et al 2012 ; Akram et al 2016 ; Shreenath et al 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of chickpea chlorotic dwarf virus and peanut witches’ broom phytoplasma associated with chickpea stunt disease and identification of new host crops and leafhopper vectors in India

2021

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An investigation was carried out to identify and characterize the phytoplasma and viruses associated with the chickpea varieties showing severe stunting, leaf reddening, yellowing and phyllody symptoms during the summer season of 2018–2019 and 2019–2020 in eight states of India. The average disease incidence was recorded from 3 to 32% in different states. The presence of chickpea chlorotic dwarf virus (CpCDV) was confirmed in thirty-seven chickpea samples by amplification of CpCDV coat protein gene and sequence comparison analysis. No record of association of luteovirus, polerovirus and cucumovirus could be detected in any of the symptomatic chickpea samples by RT-PCR assay. Brassica nigra, B. juncea, Lens culinaris, two weeds (Heteropogan contartus, Aeschynomene virginica) and one leafhopper (Amarasca biguttula) were identified as new putative hosts for CpCDV. Association of peanut witches’ broom phytoplasma was confirmed in twenty-eight chickpea samples, Sesamum indicum, five weeds hosts and two leafhopper species (Exitianus indicus, Empoasca motti) using nested PCR assays with primer pairs P1/P7 and R16F2n/R16Rn. The results of phytoplasma association in plants and leafhopper samples were further validated by using five multilocus genes (secA, rp, imp, tuf and secY) specific primers. Sequence comparison, phylogenetic and virtual RFLP analysis of 16S rRNA gene and five multilocus genes confirmed the identity of association of 16SrII-C and 16SrII-D subgroups of phytoplasmas strain with chickpea samples collected from Andhra Pradesh (AP), Telangana, Karnataka, Madhya Pradesh, Uttar Pradesh and New Delhi. Mixed infection of phytoplasma (16SrII-D) and CpCDV was also detected in symptomatic chickpea samples from AP and Telangana. The reports of association of 16SrII-C subgroup phytoplasma in chickpea and 16SrII-D subgroup phytoplasma in C. sparsiflora and C. roseus are the new host records in world and from India, respectively.

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Section: Discussionmentioning

confidence: 99%