Evidence for the anti-NAFLD effectiveness of chlorogenic acid as a HAT inhibitor using in vivo experiments supported by virtual molecular docking

Xu, Jing; Gao, Liangqin; Liang, Huiqing; Zhang, Shaoliang; Lai, Penghua; Chen, Shaodong

doi:10.1016/j.phyplu.2021.100055

Cited by 9 publications

(7 citation statements)

References 38 publications

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“…Indeed, a salicylic acid derivative, anacardic acid, acts as a histone deacetylase inhibitor in human cancer cells [ 26 ]. Another explanation could come from research on polyphenols where one of the major hydroxycinnamic acids in lettuce, chlorogenic acid, was identified as histone acetyltransferase inhibitor [ 27 ], and was shown to inhibit proliferation of colon cancer cells [ 18 ] and ameliorate other diseases [ 19 ] through micro-RNA-dependent mechanism.…”

Section: Discussionmentioning

confidence: 99%

“…It is believed that altered patterns of DNA methylation play a role in the progression of cancer and pathogenesis of other disease [ 16 ]. Interestingly, polyphenols from lettuce, iodine, and salicylic acid were all reported to exert certain epigenetic effects [ 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

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Molecular Effects of Iodine-Biofortified Lettuce in Human Gastrointestinal Cancer Cells

et al. 2022

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Considering the growing number of cancer cases around the world, natural products from the diet that exhibit potential antitumor properties are of interest. Our previous research demonstrated that fortification with iodine compounds is an effective way to improve the antioxidant potential of lettuce. The purpose of the present study was to evaluate the effect of iodine-biofortified lettuce on antitumor properties in human gastrointestinal cancer cell lines, gastric AGS and colon HT-29. Our results showed that extracts from iodine-biofortified lettuce reduce the viability and proliferation of gastric and colon cancer cells. The extracts mediated cell cycle arrest which was accompanied by inactivation of anti-apoptotic Bcl-2 and activation of caspases, as assessed by flow cytometry. However, extracts from lettuce fortified with organic forms of iodine acted more effectively than extracts from control and KIO3-enriched plants. Using quantitative PCR, we detected the increase in pro-apoptotic genes BAD, BAX and BID in AGS cells whereas up-regulation of cell cycle progression inhibitor CDKN2A and downregulation of pro-proliferative MDM2 in HT-29 cells. Interestingly, lettuce extracts led to down-regulation of pro-survival AKT1 and protooncogenic MDM2, which was consistent for extracts of lettuce fortified with organic form of iodine, 5-ISA, in both cell lines. MDM2 downregulation in HT-29 colon cancer cells was associated with RB1 upregulation upon 5-ISA-fortified lettuce extracts, which provides a link to the epigenetic regulation of tumor suppressor genes by RB/MDM2 pathway. Indeed, SEMA3A tumor suppressor gene was hypomethylated and upregulated in HT-29 cells treated with 5-ISA-fortified lettuce. Control lettuce exerted similar effects on RB/MDM2 pathway and SEMA3A epigenetic activation in HT-29 cells. Our findings suggest that lettuce as well as lettuce fortified with organic form of iodine, 5-ISA, may exert epigenetic anti-cancer effects that can be cancer type-specific.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular Effects of Iodine-Biofortified Lettuce in Human Gastrointestinal Cancer Cells

et al. 2022

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“…Chlorogenic acids exhibit a wide range of health-promoting properties ( Figure 1 ), which makes them an attractive food additive or drug. Chlorogenic acids exhibit antioxidant [ 4 , 7 , 8 , 9 , 10 ], anti-inflammatory [ 2 , 4 , 7 ], antibacterial [ 2 , 4 , 7 , 8 , 11 , 12 ], antiviral [ 7 , 13 ], anticancer [ 8 , 9 , 14 , 15 ], neuroprotective [ 3 , 4 , 16 ], and antidiabetic [ 14 , 17 ] effects, as well as positive effects against gastrointestinal diseases [ 2 , 3 , 18 , 19 ]. In addition, they can increase the number of white blood cells, lower blood pressure, and stimulate the central nervous system [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

“…According to their structure, phenols can eliminate radicals directly, through a peroxidase reaction or by forming chelates with metal ions, thus preventing Fenton-type reactions [ 20 ]. Despite their significant antioxidant properties, CQAs are also characterized by pro-oxidant properties, which depend on their concentration, the occurrence of transition metal ions, and environmental conditions (presence of oxygen molecules, high pH value) [ 7 , 19 , 20 , 21 , 22 ]. Moreover, the mechanism of the pro-oxidant action of CQAs can be based on the ability to produce reactive oxygen species (ROS), i.e., hydrogen peroxide H 2 O 2 , superoxide radicals ROO •− , and hydroxyl radicals HO •− formed in the Fenton reaction during the reduction of Fe(III) to Fe(II) [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

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Fe(III) and Cu(II) Complexes of Chlorogenic Acid: Spectroscopic, Thermal, Anti-/Pro-Oxidant, and Cytotoxic Studies

et al. 2022

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Complexes of chlorogenic acid (5-CQA) with copper(II) and iron(III) were synthesized in a solid state and examined by means of FT-IR, thermogravimetric, and elemental analyses. The molar stoichiometric ratios of metal:ligand for the solid forms of the complexes were established as Cu(II):L = 1:2 and Fe(III):L = 2:3 (L: 5-CQA), with the possible coordination through the carboxylate group and the hydroxyl group from the catechol moiety. In an aqueous solution at pH = 7.4, the composition of the complexes was Cu(II):L = 1:1, and Fe(III):L = 1:1 and 1:2. The Cu(II) and Fe(III) complexes with 5-CQA showed lower antioxidant properties, as estimated by the spectrophotometric methods with DPPH•, ABTS•+, and HO• radicals, than the ligand alone, whereas in the lipid peroxidation inhibition assay, the metal complexes revealed a higher antioxidant activity than 5-CQA. Cu(II) 5-CQA showed the highest pro-oxidant activity in the Trolox oxidation assays compared to the other studied compounds. The lipophilic parameters of the compounds were estimated using the HPLC method. 5-CQA and its complexes with Fe(III) and Cu(II) were not toxic to HaCaT cells in a tested concentration range of 0.15–1000 nM after a 24 h incubation time.

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Recent Updates on In Silico Screening of Natural Products as Potential Inhibitors of Enzymes of Biomedical and Pharmaceutical Importance

Patil

Maheshwari

et al. 2022

Natural Products as Enzyme Inhibitors

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Evidence for the anti-NAFLD effectiveness of chlorogenic acid as a HAT inhibitor using in vivo experiments supported by virtual molecular docking

Cited by 9 publications

References 38 publications

Molecular Effects of Iodine-Biofortified Lettuce in Human Gastrointestinal Cancer Cells

Molecular Effects of Iodine-Biofortified Lettuce in Human Gastrointestinal Cancer Cells

Fe(III) and Cu(II) Complexes of Chlorogenic Acid: Spectroscopic, Thermal, Anti-/Pro-Oxidant, and Cytotoxic Studies

Recent Updates on In Silico Screening of Natural Products as Potential Inhibitors of Enzymes of Biomedical and Pharmaceutical Importance

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