Evidence for Reactive Nitrogen Species Formation in the Gingivomucosal Tissue

Lohinai, Zsolt; Stachlewitz, Robert F.; Virág, László; Székely, Andrea; Haskó, György; Szabó, Csaba

doi:10.1177/00220345010800021401

Cited by 52 publications

(47 citation statements)

References 27 publications

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“…In a previous study [9], it was demonstrated that serum NO and LPO are elevated in diabetes mellitus whereas in another study [14] an inverse relationship between NO and MDA was found. These ROS are associated with the microvascular complications of diabetes.…”

Section: Discussionmentioning

confidence: 88%

Effect of Non-Surgical Therapy on Salivary Nitric Oxide and Lipid Peroxidation Levels in Type Ii Diabetic and Non Diabetic Patients With Periodontal Disease

Latha

Uppoor

Nayak

et al. 2018

Asian J Pharm Clin Res

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Objective: Reactive oxygen species have been identified as potential factors causing periodontal tissue destruction. Elevated levels of these in patients with chronic periodontitis and diabetes may aggravate the oxidative stress burden thereby accelerating the tissue damage associated with diabetes. The present study aimed to assess the effect of diabetes and periodontal disease on the oxidative stress markers and the effect of non-surgical therapy on these markers.Methods: A total of 50 participants were divided into four groups based on the selection criteria. In addition to clinical parameters and biochemical parameters (salivary nitric oxide [NO] and malondialdehyde [MDA] levels) were assessed using spectrophotometric method at baseline and 3 months after non-surgical periodontal therapy.Result: There was a statistically significant difference in the clinical parameters as well as NO and MDA levels in patients with type 2 diabetes and chronic periodontitis than other three groups at baseline and at 3 months after non-surgical periodontal therapy. Conclusion:Diabetes mellitus and periodontitis have a compounding effect on the oxidative stress. Periodontal therapy is essential for diabetic patients as it can lower the levels of oxidative damage.

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Section: Discussionmentioning

confidence: 88%

Effect of Non-Surgical Therapy on Salivary Nitric Oxide and Lipid Peroxidation Levels in Type Ii Diabetic and Non Diabetic Patients With Periodontal Disease

Latha

Uppoor

Nayak

et al. 2018

Asian J Pharm Clin Res

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“…Previous studies indicate the presence of iNOS in inflamed gingival tissue from adult periodontitis patients 19,37) . Recently, formation of reactive nitrogen species in gingival tissue was demonstrated in an animal model 38) .…”

Section: Gingival Inflammation (Bdnf)mentioning

confidence: 99%

Expression of BDNF and TrkB in Gingival Inflammation

Somei

Bhawal

2013

J. Hard Tissue Biology.

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The gingival epithelium is a physiologically important interface between the bacterially colonized gingival sulcus and periodontal soft and mineralized connective tissues which requires protection from exposure to bacteria and their products. Gingival inflammation results from the coordinated activation of cascades of signaling pathways initiated by growth factors and their receptors. Brain-derived neurotrophic factor (BDNF) and its receptor, TrkB, belong to the neurotrophin family of growth factors and mediate vital physiological functions in the brain, and they are expressed in a wide variety of non-neuronal tissues. Aberrant neurotrophin signaling underlies the pathogenesis of several inflammatory conditions, yet little is known about the role of neurotrophins in the gingival inflammation. To fill this gap in knowledge, we analyzed the patterns of BDNF and TrkB expression in the inflamed tissues of human and in experimental rat periodontitis tissues. Gingival tissues from patients with periodontitis demonstrated greater levels of BDNF and TrkB expression than the tissues from healthy individuals, while an in vivo rat study showed increased expression of BDNF and TrkB in experimental rat periodontitis tissues. Interleukin-1 (IL-1 ), a pro-inflammatory cytokine, elevated the expression of BDNF and TrkB mRNAs in human gingival fibroblast (HGF) and human gingival epithelial (HGE) cells. Inducible NOS (iNOS) mRNA expression was elevated in HGF cells that were stimulated by IL-1 . Also, IL-1 stimulated protein expression of BDNF and iNOS in HGF cells. ELISA confirmed that the expression of BDNF by HGF was upregulated in the presence of IL-1 . Addition of S-Methylisothiourea Sulphate (SMT), a specific inhibitor of iNOS, profoundly reduced the production of BDNF in HGF. Together, these results support a role for BDNF as a new modulator of gingival inflammation. Gingival fibroblast cells within inflammatory environment can significantly increase the production of neurotrophic factors, which may be involved with the anti-inflammatory mechanisms.

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“…The proportion of immunopositive cells from the total number of inflammatory cells was expressed as a percentage. Measurements were performed 3 times in representative sections [15,21,22] . We used an Olympus BX51 microscope with an Olympus SP 350 digital camera.…”

Section: Histopathological and Immunohistochemical Analysismentioning

confidence: 99%

“…Serum samples were passed through 10-kDa filters (Sartorius AG, Goettingen, Germany) and deproteinized by methanol/diethyl ether (3/1, v/v) (sample: methanol/diethyl ether, 1:9, v/v) [22] . In brief, 100 μL of VCl 3 (8 mg/mL) was added to 100 μL of the supernatant for the reduction of nitrate to nitrite, followed by the addition of the Griess reagents, 50 μL of SULF (2%) and 50 μL of NEDD (0.1%).…”

Section: Serum Nitric Oxide Evaluationmentioning

confidence: 99%

Efficacy of subantimicrobial-dose doxycycline against nitrosative stress in chronic periodontitis

et al. 2012

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Aim: To evaluate the effectiveness of subantimicrobial-dose doxycycline (SDD) as an adjunct to scaling and root planing (SrP) treatment against the nitrosative stress of moderate to advanced chronic periodontitis. Methods: Adults with untreated chronic periodontitis (n=174) were randomly administered SrP+SDD (n=87) (20 mg of doxycycline twice daily) or SrP+placebo (n=87) treatment for 3 months. At baseline and after 3 months, the probing depths (PD), bleeding on probing (bOP) and clinical attachment level (CAl) were measured, and a gingivomucosal biopsy was collected to assay the induction of nitric oxide synthase (inOS) and 3-nitrotyrosine (3nT), and blood was collected to assay for total nitrites and nitrates (nO x ) and 3nT. Results: Compared to baseline, at the completion of treatment, significant decreases in the levels of tissue inOS and 3nT and serum nO x and 3nT were observed in both groups. SrP+SDD yielded a greater reduction in the gingivomucosal and serum nitrosative stress markers than did SrP+placebo. PD, bOP, and CAl reduction were correlated with the nitrosative stress parameters. Conclusion: On a short-term basis, SDD therapy may be used as an adjunct to SrP treatment against nitrosative stress in moderate to advanced chronic periodontitis.

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Evidence for Reactive Nitrogen Species Formation in the Gingivomucosal Tissue

Cited by 52 publications

References 27 publications

Effect of Non-Surgical Therapy on Salivary Nitric Oxide and Lipid Peroxidation Levels in Type Ii Diabetic and Non Diabetic Patients With Periodontal Disease

Effect of Non-Surgical Therapy on Salivary Nitric Oxide and Lipid Peroxidation Levels in Type Ii Diabetic and Non Diabetic Patients With Periodontal Disease

Expression of BDNF and TrkB in Gingival Inflammation

Efficacy of subantimicrobial-dose doxycycline against nitrosative stress in chronic periodontitis

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