Evidence for Papillomavirus Genus-Specific Antigens and DNA in Laryngeal Papilloma

Lancaster, Wayne D.; Jenson, A. Bennett

doi:10.1159/000149233

Cited by 55 publications

(14 citation statements)

References 10 publications

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“…In three of the four positive specimens, only a small number ofcells contained detectable antigen, a finding that is in agreement with previous reports (10)(11)(12)(13). Only isolated individual groups of less than 10 cells were found to be antigen-positive.…”

Section: Resultssupporting

confidence: 92%

“…In the positive cases it was not possible to identify the specific viral serotype present in the lesions because the antiserum is crossreactive with capsid antigens of all papillomaviruses. Despite attempts to isolate papillomavirus or detect the papillomavirus genome in juvenile-onset laryngeal papillomas (13)(14)(15)(16)(17), the etiological agent has not been characterized.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Viral etiology of juvenile- and adult-onset squamous papilloma of the larynx.

Mounts¹,

Shah²,

Kashima³

1982

Proc. Natl. Acad. Sci. U.S.A.

307

140

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Juvenile-and adult-onset laryngeal papillomas were examined for the presence of a human papillomavirus (HPV) genome and capsid antigens. DNA was isolated from a portion of tissue removed for therapeutic purposes, and the presence of a papillomavirus genome was detected by Southern transfer analysis. The viral DNA found in the 12 juvenile-onset and the 8 adultonset laryngeal papillomas examined was identified as HPV-6 on the basis of size, restriction endonuclease digestion patterns, and homology detected under stringent conditions. Restriction endonuclease analysis of the viral genomes revealed at least four different subtypes, designated HPV-6c through HPV-6f. The most common subtype, HPV-6c, was detected in over half of the papillomas studied, including both juvenile and adult types. The remaining tissue was fixed and processed for immunocytochemistry. The immunoperoxidase technique was used with an antiserum that reacts with capsid antigen(s) common to all HPV serotypes. HPV antigen was found in two of the juvenile-onset papillomas and two of the adult-onset papillomas. The antigen was localized to the nucleus and was distributed in the superficial layers of the epithelium. HPV capsid antigen had not previously been detected in cases of adult-onset papilloma, and the HPV genome in both juvenile-and adult-onset laryngeal papillomas had not been characterized. Despite the absence of detectable viral antigen in most of the specimens examined, the presence of the HPV genome provides strong evidence for the papillomavirus etiology of these tumors.Squamous papillomas ofthe larynx are a serious clinical problem because of their location, their resistance to treatment, their relentless recurrence, and their tendency to spread throughout the respiratory tract (1, 2). The most frequent age of onset is in the first five years oflife, but the disease also occurs in adults.Papillomas of the larynx are tumors of lobulated or papillary form in which each projection has a central core of connective tissue covered with squamous epithelium (3). They histologically resemble cutaneous warts, whose papillomavirus etiology has been established (for review, see ref. 4). Although a papillomavirus etiology has been implicated for laryngeal papillomas, there have been conflicting reports on the presence of virus particles in these tumors when examined by electron microscopy (5-8). More recently, papillomavirus capsid antigen has been detected in juvenile-onset laryngeal papillomas by the immunoperoxidase technique of Sternberger (9), using an antiserum that reacts with genus-specific antigens (10-12). In these studies, papillomavirus antigen was detected in less than half ofthe juvenile-onset laryngeal papillomas. In the positive cases it was not possible to identify the specific viral serotype present in the lesions because the antiserum is crossreactive with capsid antigens of all papillomaviruses. Despite attempts to isolate papillomavirus or detect the papillomavirus genome in juvenile-onset laryngeal papillomas (13-17), ...

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Section: Resultssupporting

confidence: 92%

mentioning

confidence: 99%

Viral etiology of juvenile- and adult-onset squamous papilloma of the larynx.

Mounts¹,

Shah²,

Kashima³

1982

Proc. Natl. Acad. Sci. U.S.A.

307

140

View full text Add to dashboard Cite

show abstract

“…13 Reactivity with this antiserum has been demonstrated against several types of human and animal papillomaviruses.…”

Section: Methodsmentioning

confidence: 96%

Cutaneous Neoplasms in Dogs Associated with Canine Oral Papillomavirus Vaccine

et al. 1987

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Abstract.A spectrum of proliferative cutaneous lesions occurred in 12 dogs at the injection site of live canine oral papillomavirus (COP) vaccine, suggesting a viral etiology for the masses. Lesions included epidermal hyperplasia, epidermal cysts, squamous papilloma, basal cell epithelioma, and squamous cell carcinoma. Peroxidase-antiperoxidase staining of tumor sections revealed nuclei which stained for group-specific papillomavirus antigen in five of 12 masses. Electron microscopic examination of tumor sections did not reveal virions. In transmission studies, macerated tumor tissue did not produce oral papillomas on the scarified mucosa of puppies; this procedure did protect the puppies from development of lesions when challenged with infectious papilloma material. These findings are evidence that COP can induce hyperplastic and neoplastic lesions in sites other than oral, pharyngeal, and ocular mucosa.

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“…Analysis of late gene products of papillomaviruses to date has relied largely on a commercially available antiserum (Dako, Copenhagen, Denmark), raised against detergent-disrupted BPV virions. The antiserum reacts with capsid antigens from a broad range of papillomavirus types, staining the nuclei of those cells in the superficial layers of a lesion which are producing virus particles (Jenson et al, 1980(Jenson et al, , 1982Lancaster & Jenson, 1981). However, specific reagents for individual HPV types are necessary, both for diagnostic 0000-8201 © 1988 SGM H. M. BROWNE AND OTHERS use and to allow analysis of the HPV structural polypeptides, particularly since most HPVcontaining lesions are a poor source of virus particles.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the L1 Gene Product of Human Papillomavirus Type 16 by Expression in a Vaccinia Virus Recombinant

Browne

Churcher

Stanley

et al. 1988

Journal of General Virology

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SUMMARYThe L1 open reading frame of human papillomavirus type 16 (HPV16) has been expressed in vaccinia virus under the control of both the 7.5K early and late promoter, and the 4b major late promoter. Antibodies to a fl-galactosidase fusion protein containing a C-terminal portion of the HPV 16 L1 gene product were used to compare the levels of L1 expression in the two recombinants, and showed that greater levels of expression were obtained when the gene was placed under the control of the 4b late promoter. Immunofluorescence studies revealed a nuclear location of the L1 gene product when expressed in vaccinia virus. Antibodies to the fl-galactosidase fusion protein detected a major polypeptide species of 57K and a minor species of 64K in Western blots of recombinant-infected cell lysates. The 64K species was not detected when cells were infected in the presence of tunicamycin, indicating that the primary translation product of the HPV16 L1 open reading frame is modified by N-linked glycosylation when expressed in vaccinia virus. Whereas antibodies to HPV16 L1 fusion proteins and to a peptide containing amino acids from the C terminus of HPV16 L1 reacted well in Western blots with the HPV 16 L1 target expressed in vaccinia virus, no reactivity was observed with antibodies to bovine papillomavirus type 1 particles or to a HPV6b fusion protein.

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Evidence for Papillomavirus Genus-Specific Antigens and DNA in Laryngeal Papilloma

Cited by 55 publications

References 10 publications

Viral etiology of juvenile- and adult-onset squamous papilloma of the larynx.

Viral etiology of juvenile- and adult-onset squamous papilloma of the larynx.

Cutaneous Neoplasms in Dogs Associated with Canine Oral Papillomavirus Vaccine

Analysis of the L1 Gene Product of Human Papillomavirus Type 16 by Expression in a Vaccinia Virus Recombinant

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