Evidence for new nuclear and mitochondrial genome organizations among high-frequency somatic embryogenesis-derived plants of allotetraploid Coffea arabica L. (Rubiaceae)

Rani, V.; Singh, Kritika; Shiran, Behrouz; Nandy, Samapika; Goel, Shailendra; Devarumath, R. M.; Sreenath, H. L.; Raina, S. N.

doi:10.1007/s002990000228

Cited by 53 publications

(27 citation statements)

References 28 publications

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“…ISSR fingerprinting is a very potent tool to compare genomes for their identity. In some cases it is also possible to identify even minor somaclonal variations among the individuals (Rani et al 2000;Li and Ge, 2001). However epigenetic variation related to change in DNA structure or methylation could not be explained by ISSR markers.…”

Section: Resultsmentioning

confidence: 99%

Isolation and characterization of hydrocarbon producing green alga Botryococcus braunii from Indian freshwater bodies

Dayananda¹,

Ravi²,

Kumar³

et al. 2007

Electron. J. Biotechnol.

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Abbreviations:FAME: fatty acid methyl esters ISSR: inter simple sequence repeats NOX: oxides of nitrogen SEM: scanning electron microscopy SOX: oxides of sulphur Samples of green colonial unicellular microalgaBotryococcus braunii were collected from Bear Shola Falls at Kodaikanal (latitude 10.31 N and longitude 77.32 E), Tamil Nadu, India. Specimens were isolated, cultured and examined for its hydrocarbon content, morphological features and DNA structural resemblance with the known strain to confirm its identity. Inter simple sequence repeats (ISSR) finger printing revealed strong genetic similarity among the authentic strain (B. braunii N-836) and the Indian isolated strain (B. braunii CFTRI-Bb1) from *Corresponding author Kodaikanal. The type of hydrocarbons produced by the Kodaikanal isolates were analyzed and identified as saturated hydrocarbons in the range of C 21 to C 33 by GCMS. Tetracosane and octacosane were found as the major components among the saturated hydrocarbons produced by this alga, constituting 17.6% and 14.8% respectively. Hydrocarbon content of the organism was in the range of 13-18% of its dry biomass. The fat content of the organism was found to be 22% (w/w). Palmitic and oleic acids were found to be major fatty acids produced by the alga. Lutein and β-carotene were Dayananda, C. et al. 2found to be the major carotenoids and constituted about 64.1% and 25.1% respectively of the total carotenoids. Based on ISSR finger printing and hydrocarbon analyses the new algal strain from Kodaikanal was identified as Botryococcus braunii.The green colonial hydrocarbon rich unicellular microalgae Botryococcus braunii (Banerjee et al. 2002;Metzger and Largeau, 2005) is widespread in freshwater, brackish lakes, reservoirs and ponds. It is also widely distributed in reservoirs at temperate, tropical and arctic latitudes (Tyson, 1995). It is recognized as one of the potent renewable resource for production of liquid hydrocarbons. B. braunii is classified into A, B and L races based on the type of hydrocarbons produced (Metzger and Largeau, 2005). Race -A produces C 21 to C 33 odd numbered n-alkadienes, mono-, tri-, tetra-, and pentaenes and they are derived from fatty acids (Banerjee et al. 2002;Metzger et al. 2005). The L race yields a single C 40 isoprenoid hydrocarbon, lycopa -14(E),18(E)-diene (Metzger et al. 1990). The B race produces two types of triterpenes called botryococcenes of C 30 -C 37 of general formula C n H 2n-10 as major hydrocarbons and small amounts of methyl branched squalene. Certain strains of the B race also biosynthesise cyclobotryococcenes (David et al. 1988;Achitouv et al. 2004). Also a feature common to all three races is the presence of a highly aliphatic, non-hydrolysable and insoluble biomacromolecule (algaenan) found in their outer cell walls (Audino et al. 2002). The highly resistant nature of the B. braunii algaenan to degradation allows it to be selectively preserved during fossilisation, leading to fossil B. braunii remains, a major contributor to a number of high o...

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Section: Resultsmentioning

confidence: 99%

Isolation and characterization of hydrocarbon producing green alga Botryococcus braunii from Indian freshwater bodies

Dayananda¹,

Ravi²,

Kumar³

et al. 2007

Electron. J. Biotechnol.

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“…ISSR has several advantages, particularly in reproducibility and higher informative nature (Nagaoka and Ogihara 1997). ISSR has been successfully used to detect variations in several plants including Coffea arabica (Rani et al 2000), tea (Devarumuth et al 2002), banana (Ray et al 2006), Codonopsis lanceolate (Guo et al 2006a) and Robinia ambigura (Guo et al 2006b). …”

Section: ⎯⎯⎯⎯mentioning

confidence: 98%

Assessment of genetic stability of in vitro grown Dictyospermum ovalifolium

Murugaiah¹,

Thoyajaksha²,

Ravishankar³

et al. 2008

Biol. plant.

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In the present study, a polymerase chain reaction (PCR)-based method namely inter simple sequence repeat (ISSR) was employed to assess genetic stability in tissue culture-derived Dictyospermum ovalifolium plantlets. To study genomic stability of micropropagated plants, 14 individuals were randomly tagged among a population of 2500 regenerants and were compared with single donor mother plant. A total of 51 clear and reproducible bands ranging from 200 bp to 2.1 kb were scored corresponding to an average of 3.64 bands per primer. Two of the 51 bands were polymorphic (3.92 %) among 14 individuals, thus indicating the occurrence of low level genomic variation in the micropropagated plants. Cluster analysis indicates that genetic similarity values were 0.978 which allows classification of the plants to distinct groups. Further an attempt was made to reintroduce the micropropagated plants into their natural habitat. Over one thousand six hundred fifty plants were successfully established.

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“…Similarly, Devarumath et al (2002) did not detect any polymorphism in plastid genome of the micropropagated plants of three tea clones. The chloroplast genome has been found to remain stable also in regenerants of Coffea arabica (Rani et al 2000) and F. vulgare (Bennici et al 2004). Only few studies have detected structural changes in the plastid genome.…”

Section: Discussionmentioning

confidence: 96%

“…By contrast, both genic and chromosomic mutations like inversion, deletion or translocation can be detected by molecular markers. Since mutations could occur both on nuclear DNA and on mitochondrial (mt) or chloroplast (cp) DNA (Kawata et al 1995;Rani et al 2000), various DNA markers like RAPD (random amplified polymorphic DNA), ISSR (inter simple sequence repeat) for nuclear genome screening (Gostimsky et al 2005), and RFLP (random fragment length polymorphism) for both nuclear and organellar genome screening (Devarumath et al 2002) are required to assess the genetic stability of somaclones. The occurrence of somaclonal variation is influenced by several factors such as explant type, culture medium, age of donor plant, number of subcultures and tissue culture system itself.…”

Section: Introductionmentioning

confidence: 99%

Genetic stability at nuclear and plastid DNA level in regenerated plants of Solanum species and hybrids

et al. 2008

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In this work we detected the extent of variability at nuclear and cytoplasmic DNA level of regenerated plants belonging to Solanum genotypes with a different genetic background and somatic chromosome number. As for the nuclear characterization, a total of 66 (18.5%) polymorphic bands were scored using 13 ISSR primers on 45 randomly selected regenerants. Our results show that the regenerants obtained from clone cmm 1T and, at lower level, those from cph 1C are unstable under in vitro conditions or rather more prone to in vitro- induced stress leading to somaclonal variation than the other genotypes used. Two types of changes were observed: disappearance of parental ISSR fragments, termed ‘‘loss’’; appearance of novel ISSR fragments, termed ‘‘gain’’. The most frequent event occurring in the regenerants was the loss of fragments (41 bands). Regenerated plants were analyzed with seven plastid universal primers to determine the cytoplasmic composition at chloroplast level. All cpDNA primer pairs tested produced amplicons of the same size in all genotypes analyzed and no polymorphic fragments were observed with any universal primers used. Our results show that under in vitro culture conditions genotype affects the integrity of the genome. In addition, the absence of polymorphism at plastid level confirms the greater genetic stability of cytoplasmic DNA

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Evidence for new nuclear and mitochondrial genome organizations among high-frequency somatic embryogenesis-derived plants of allotetraploid Coffea arabica L. (Rubiaceae)

Cited by 53 publications

References 28 publications

Isolation and characterization of hydrocarbon producing green alga Botryococcus braunii from Indian freshwater bodies

Isolation and characterization of hydrocarbon producing green alga Botryococcus braunii from Indian freshwater bodies

Assessment of genetic stability of in vitro grown Dictyospermum ovalifolium

Genetic stability at nuclear and plastid DNA level in regenerated plants of Solanum species and hybrids

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