Evidence for multiple mitochondrial lineages of Fasciola hepatica (liver fluke) within infrapopulations from cattle and sheep

Walker, Stephen; Prodöhl, Paulo A.; Fletcher, Hugh; Hanna, R.E.B.; Kantzoura, Vaia; Hoey, Elizabeth M.; Trudgett, Alan

doi:10.1007/s00436-006-0440-4

Cited by 50 publications

(56 citation statements)

References 38 publications

(44 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although genetic variation within F. hepatica sequences of the mtDNA and ribosomal regions has been reported previously from several countries (Itagaki et al 2005a,b;Lin et al 2007;Walker et al 2007;Ali et al 2008;Erensoy et al 2009;Peng et al 2009;Amer et al 2011), limited information is known about intraspecific genetic differences in Iranian F. hepatica isolates. Among the published cases from Asian countries in the last 25 years, most are from Iran, Vietnam, and China and fewer cases have been reported from Turkey, Korea, Japan, Thailand, India, Iraq, Pakistan, Yemen, Israel, and Saudi Arabia (Mas-Coma et al 2009;Amor et al 2011).…”

Section: Discussionmentioning

confidence: 96%

Characterization of Fasciola hepatica genotypes from cattle and sheep in Iran using cytochrome C oxidase gene (CO1)

2011

View full text Add to dashboard Cite

The present study compared the genetic variation among 19 different isolates of Fasciola hepatica from cattle and sheep in different areas of Iran using sequence data for mitochondrial DNA gene, the subunit 1 of cytochrome C oxidase gene (CO1). Four different CO1 genotypes were detected among F. hepatica isolates that showed five variable nucleotide positions (accession nos.; GQ398051, GQ398052, GQ398053, GQ398054). Nucleotide sequence variation among 19 isolates for CO1 analyzed in this study ranged from 0% to 0.98% in Iran. Among the five polymorphism sites identified in this study, only one (T to G at position 51 in 5'end of GQ175362) resulted in putative amino acid alteration of phenylalanine (TTT) to leucine (TTG) in CO1. A phylogenetic analysis of the sequence data revealed that host associations and geographic location are likely not useful markers for Fasciola genotype classification. In addition, morphological analysis showed that the ratios of body length and body width of some (n = 5) of the 19 examined F. hepatica isolates were intermediate between F. hepatica and Fasciola gigantica, representing the substantial polymorphism of the F. hepatica species and the difficulty in the accurate recognition based on morphological features. In conclusion, Iranian F. hepatica exhibited the presence of considerable genetic diversity at CO1.

show abstract

Section: Discussionmentioning

confidence: 96%

Characterization of Fasciola hepatica genotypes from cattle and sheep in Iran using cytochrome C oxidase gene (CO1)

2011

View full text Add to dashboard Cite

show abstract

“…Marcilla et al (2002) (Huang et al, 2003), ITS 1 (internal transcribed spacer 1) of rDNA or cox1 (cytochrome c oxidase I) mitochondrial gene (Hashimoto et al, 1997) as genetic markers. Through PCR-RFLP analysis Walker et al (2007) have defined for F. hepatica three mitochondrial DNA (mtDNA) regions with highest polymorphism frequency. Authors also through PCR-RFLP established 52 different complex haplotypes specific for particular geographic isolates and/or hosts.…”

Section: Pcr-rflp (Restriction Fragment Length Polymorphism)mentioning

confidence: 99%

“…As a conservative molecule not all of the gene regions of liver fluke mtDNA are "plastic" and therefore informative enough for population structuring, phylogenetic and genetic diversity studies. Three areas of it have been defined as potentially most polymorphic (FhmtDNACOX3/ND4, FhmtDNAATP6/ND1 и FhmtDNACOX1/l-rRNA) (Figure 3) (Walker et al, 2007). Fig.…”

Section: Mitochondrial Dna Regions Used For Snp Assaymentioning

confidence: 99%

Issues Associated with Genetic Diversity Studies of the Liver Fluke, Fasciola Heptica (Platyhelminthes, Digenea, Fasciolidae)

Teofanova¹,

Hristov²,

Yoveva³

et al. 2012

Genetic Diversity in Microorganisms

View full text Add to dashboard Cite

“…The hypothesis of genetic variations inside the F. hepatica species justifies the development of recent studies. Walker et al (2007) examined the genetic diversity of mitochondrial haplotypes of infra-populations of flukes from cattle or sheep and showed that a herbivore could be infected by at least 10 different haplotypes of the parasite in less than one year. The main consequence of this high level of diversity is the frequent probability of development of resistance to anthelmintics and fast adaptation to climatic changes.…”

Section: Diversity Of Parasites In the Paragonimus Genusmentioning

confidence: 99%

Biodiversity of flukes

Dreyfuss

Rondelaud

2008

Parasite

View full text Add to dashboard Cite

Summary :As many others parasites, speciation of flukes depends on the genetic characteristics and on ploidia. Ploidia of flukes can be different in a same species. In Asia, diploid, triploid and hybrid (2n/3n) populations are encountered. The comparison of morphological parameters between diploid and triploid flukes showed that they were morphologically different. Nevertheless, a genetic relationship between parthenogenetic organisms would exist regardless of their ploidia. In the Fasciola genus, the main consequence of the high level of diversity is the frequent probability of development of resistance to anthelmintics and fast adaptation to climatic changes. In the Paragonimus genus, diversity can enhance different forms of pathogenicity, can also be related to the species of intermediate hosts, and to the definitive host. The strain of flukes plays a part in the visceral localization of P. westermani adults. DIFFICULTY OF SPECIATION IN MEDICAL PARASITOLOGYPLOIDIA P arasitology is a biological field in which taxonomy often still uses morphological and Linnean criteria. The limits of the Linnean descriptive methods were reached many decades ago: the dilemma Entamoeba histolytica/E. dispar is a demonstrative example. Similarly, the infra-specific relationships between biogeography of parasites and their biological characteristics (rhythm, pathology, reservoirs) were used to organize the taxonomy of genetically different parasitic populations (Wuchereria bancrofti, for instance). Genetic diversity cannot yet be evaluated by easy and low cost methods. The results of the biological diagnosis of parasitic diseases tend to be less precise, and decrease at the genus level (E. histolytica/E. dispar), or even the family (Opisthorchiidae, Ancylostomatidae, Paragonimidae). Protozoa are parasites for which genetic diversity can be evaluated quite easily. Helminths, particularly Digenea, cannot be analyzed easily, because many species have a variable ploidia, which modifies their morphology and DNA size. A comparative morphometric study, by light microscopy, of eggs and adults from three populations with different sizes of Fasciola gigantica, and the morphology of adults, under scanning electron microscopy, showed variations in the sizes of eggs and adults, whereas the surface was similar using electronic morphology (Srimuzipo et al., 2000). The observations on mitotic metaphase chromosomes revealed a diploid type (2n = 20) in all three size-races. The speciation of Digenea can no longer be evaluated by simple morpholo-

show abstract

Evidence for multiple mitochondrial lineages of Fasciola hepatica (liver fluke) within infrapopulations from cattle and sheep

Cited by 50 publications

References 38 publications

Characterization of Fasciola hepatica genotypes from cattle and sheep in Iran using cytochrome C oxidase gene (CO1)

Characterization of Fasciola hepatica genotypes from cattle and sheep in Iran using cytochrome C oxidase gene (CO1)

Issues Associated with Genetic Diversity Studies of the Liver Fluke, Fasciola Heptica (Platyhelminthes, Digenea, Fasciolidae)

Biodiversity of flukes

Contact Info

Product

Resources

About