Evidence for Gradients of Gene Expression Correlating with Zonal Topography of the Olfactory Sensory Map

Norlin, Erik; Alenius, Mattias; Gussing, Fredrik; Hägglund, Maria; Vedin, Viktoria; Bohm, Staffan

doi:10.1006/mcne.2001.1019

Cited by 110 publications

(90 citation statements)

References 53 publications

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“…Several other molecules show zonal restriction patterns in addition to the odorant receptors. For example, cytoprotective enzymes are found in the dorsomedial-most zone (zone 1), whereas the guidance molecule Npn2 and transcription factors Msx1 and Eya2 increase in a gradient ventrolaterally through zones 2-4 (Norlin et al, 2001;Oka et al, 2003;Tietjen et al, 2003;Gussing and Bohm, 2004).…”

Section: Zonal Restriction Of Foxg1 Expression In the Olfactory Epithmentioning

confidence: 99%

“…The mammalian olfactory epithelium displays a striking spatial organization, such that odorant receptors and other molecular markers are restricted to circumscribed zones that radiate along the dorsomedial-ventrolateral axis (Ressler et al, 1993;Vassar et al, 1993;Alenius and Bohm, 1997;Yoshihara et al, 1997;Norlin et al, 2001;Oka et al, 2003;Gussing and Bohm, 2004;Miyamichi et al, 2005;Tietjen et al, 2005). Dorsoventral organization within the olfactory epithelium plays an essential role in odorant receptor choice as well as in guiding topographic projections of ORN axons to the olfactory bulb (Miyamichi et al, 2005;Imai and Sakano, 2007).…”

Section: Regional Identity In the Olfactory Epitheliummentioning

confidence: 99%

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Foxg1Is Required for Development of the Vertebrate Olfactory System

Duggan

DeMaria²,

Baudhuin³

et al. 2008

J. Neurosci.

107

102

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Illuminating the molecular identity and regulation of early progenitor cells in the olfactory sensory epithelium represents an important challenge in the field of neural development. We show in both mouse and zebrafish that the winged helix transcription factor Foxg1 is expressed in an early progenitor population of the olfactory placode. In the mouse, Foxg1 is first expressed throughout the olfactory placode but later becomes restricted to the ventrolateral olfactory epithelium. The essential role of Foxg1 in olfactory development is demonstrated by the strikingly severe phenotype of Foxg1 knock-out mice: older embryos have no recognizable olfactory structures, including epithelium, bulb, or vomeronasal organs. Initially, a small number of olfactory progenitors are specified but show defects in both proliferation and differentiation. Similarly, antisense RNA knockdown of Foxg1 expression in the zebrafish shows a reduction in the number of neurons and mitotic cells in olfactory rosettes, mirroring the phenotype seen in the mouse Foxg1 null mutant. Using mosaic analysis in the zebrafish, we show that Foxg1 is required cell-autonomously for the production of mature olfactory receptor neurons. Therefore, we identified an evolutionarily conserved requirement for Foxg1 in the development of the vertebrate olfactory system.

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Section: Zonal Restriction Of Foxg1 Expression In the Olfactory Epithmentioning

confidence: 99%

Section: Regional Identity In the Olfactory Epitheliummentioning

confidence: 99%

Foxg1Is Required for Development of the Vertebrate Olfactory System

Duggan

DeMaria²,

Baudhuin³

et al. 2008

J. Neurosci.

107

102

View full text Add to dashboard Cite

show abstract

“…Previous research has defined the presence of a sharp boundary between the dorsomedial epithelium and the ventrolateral epithelium via the expression of the Ig superfamily member olfactory cell adhesion molecule/mammalian homolog 6 fasciclin II (OCAM/mamFas II) or anonymous antigens such as that recognized by the monoclonal antibody CC2 (Schwob andGottlieb, 1986, 1988;Schwarting and Crandall, 1991;Miyawaki et al, 1996;Yoshihara et al, 1997;Norlin et al, 2001). Our results do not contradict the existence of a boundary delimited by differential expression of these markers; however, we do find that the some of Figure 10.…”

Section: Or-positive Osn Distribution Patterns In Normal Oementioning

confidence: 99%

“…The steady shifting in the position of the expression territory could be accommodated by a gradient mechanism around the circumference of the epithelium. The distinction is not trivial, because molecules that are purported to match the conventional zonal boundaries have been proposed as candidate participants in the process of zone formation (Norlin et al, 2001). …”

Section: Or-positive Osn Distribution Patterns In Normal Oementioning

confidence: 99%

Odorant Receptor Expression Patterns Are Restored in Lesion-Recovered Rat Olfactory Epithelium

Iwema¹,

Fang²,

Kurtz³

et al. 2004

J. Neurosci.

133

112

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Lesions of the olfactory periphery provide a means for examining the reconstitution of a diverse and highly regulated population of sensory neurons and the growth, en masse, of nascent axons to the bulb. The olfactory epithelium and its projection onto the bulb are reconstituted after ablation by methyl bromide gas, and some measure of olfactory function is restored. The extent to which the system regenerates the full repertoire of odorant receptor-expressing neurons, particularly their spatially restricted distribution across the epithelial sheet, is unknown, however, and altered odorant receptor expression might contribute to the persistent distortion of odorant quality that is observed in the lesioned-recovered animals. To address the question of receptor expression in the recovered epithelium, we performed in situ hybridization with digoxigenin-labeled riboprobes for eight odorant receptors on the olfactory epithelium from unilaterally methyl bromide-lesioned and control rats. The data demonstrate that the distribution of sensory neuron types, as identified and defined by odorant receptor expression, is restored to normal or nearly so by 3 months after lesion. Likewise, the numbers of probe-labeled neurons in the lesioned-recovered epithelium are nearly equivalent to the unlesioned side at this time. Finally, our evidence suggests that odorant receptors are distributed in multiple overlapping bands in the normal, unlesioned, and lesioned-recovered epithelium rather than in the conventionally accepted three or four zones. Thus, the primary sensory elements required for functional recovery of the olfactory system after damage are restored, and altered function implies the persistence of a more central failure in regeneration.

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“…In this model, the collective expression patterns of these guidance cues would form a spatial map within the bulb, molecularly distinguishing potential paths and substrates encountered by OSNs. However, although it has become increasingly clear that a number of molecules have been identified that are differentially expressed by OSNs [for e.g., along the path that OSNs traverse to the bulb (2)(3)(4) and by ensheathing cells (5-7)], efforts to identify spatially restricted molecules within the bulb itself have been generally unsuccessful (8,9), greatly impeding our understanding of how olfactory axons select their partner glomeruli. Indeed, the most plausible models of olfactory development assume that spatially restricted cues exist within the olfactory bulb to guide the sensory axons to their targets (1,10).…”

mentioning

confidence: 99%

Spatial patterns of gene expression in the olfactory bulb

Lin

Yang

Scolnick

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

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How olfactory sensory neurons converge on spatially invariant glomeruli in the olfactory bulb is largely unknown. In one model, olfactory sensory neurons interact with spatially restricted guidance cues in the bulb that orient and guide them to their target. Identifying differentially expressed molecules in the olfactory bulb has been extremely difficult, however, hindering a molecular analysis of convergence. Here, we describe several such genes that have been identified in a screen that compiled microarray data to create a three-dimensional model of gene expression within the mouse olfactory bulb. The expression patterns of these identified genes form the basis of a nascent spatial map of differential gene expression in the bulb

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Evidence for Gradients of Gene Expression Correlating with Zonal Topography of the Olfactory Sensory Map

Cited by 110 publications

References 53 publications

Foxg1Is Required for Development of the Vertebrate Olfactory System

Foxg1Is Required for Development of the Vertebrate Olfactory System

Odorant Receptor Expression Patterns Are Restored in Lesion-Recovered Rat Olfactory Epithelium

Spatial patterns of gene expression in the olfactory bulb

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