Spatial patterns of gene expression in the olfactory bulb

Abstract

How olfactory sensory neurons converge on spatially invariant glomeruli in the olfactory bulb is largely unknown. In one model, olfactory sensory neurons interact with spatially restricted guidance cues in the bulb that orient and guide them to their target. Identifying differentially expressed molecules in the olfactory bulb has been extremely difficult, however, hindering a molecular analysis of convergence. Here, we describe several such genes that have been identified in a screen that compiled microarray data to create a three-dimensional model of gene expression within the mouse olfactory bulb. The expression patterns of these identified genes form the basis of a nascent spatial map of differential gene expression in the bulb

Proteomic atlas of the human olfactory bulb

“…Due to that OB is anatomically well-demarcated area with different spatial patterns of gene expression, [67] the development of specific isolation and purification protocols of single-cell types from OB, [68,69] together with novel developments in shotgun proteomic approaches, [70] would allow to explore the transcriptome and proteome profiling of each olfactory cell population individually, [71] increasing the molecular knowledge of the OB.…”

Novel subdomains of the mouse olfactory bulb defined by molecular heterogeneity in the nascent external plexiform and glomerular layers

“…A modified T7 RNA polymerase amplification protocol was performed using an optimized T7 primer [34]. First-round aRNA was tailed with poly(A) polymerase, providing a template for T7-d(T) primer and the generation of a second round of aRNA.…”

Gene expression profiles of rat olfactory bulb at developmental stage

“…This method facilitates investigations for the changes of a large number of mRNA simultaneously (Li et al, 2004;Zirlinger, 2003). So far, a few studies about olfactory bulb have been done using microarray, but the comparison of whole gene of olfactory bulb at two different developing stages has not been done yet (Lin et al, 2004, Schaar et al, 1996Watson et al, 1990).…”